A5055833196- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Cancer Genomics and Diagnostics
- Bacterial Genetics and Biotechnology
- Microtubule and mitosis dynamics
- Cancer-related Molecular Pathways
University of Copenhagen
2022-2025
Novo Nordisk Foundation
2023-2024
It has been proposed that ATR kinase senses the completion of DNA replication to initiate S/G2 transition. In contrast this model, we show here TRESLIN-MTBP complex prevents a premature entry into G2 from early S-phase independently ATR/CHK1 kinases. acts transiently at pre-replication complexes (preRCs) origin firing and is released after subsequent recruitment CDC45. This dynamic behavior implements monitoring system checks activation forks rate prevent G2. detects decline in number...
Abstract Translesion DNA synthesis (TLS) is an essential process that allows cells to bypass lesions encountered during replication and emerging as a primary target of chemotherapy. Among vertebrate polymerases, polymerase kappa (Pol() has the unique ability minor groove adducts in vitro. However, Pol(is also required for overcome major but basis this requirement unclear. Here, we combine CRISPR base editor screening technology human with TLS analysis defined Xenopus egg extracts unravel...
Abstract Accurate genome duplication requires a tightly regulated DNA replication program, which relies on the fine regulation of origin firing. While molecular steps involved in firing have been determined predominantly budding yeast, complexity this process human cells has yet to be fully elucidated. Here, we describe straightforward proteomics approach systematically analyse protein recruitment chromatin during induced cells. Using specific inhibitor against CHK1 kinase, synchronised wave...