A5074851327- CRISPR and Genetic Engineering
- Ubiquitin and proteasome pathways
- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
- Reproductive Biology and Fertility
- Chromosomal and Genetic Variations
- Genetics, Aging, and Longevity in Model Organisms
- Biochemical and Molecular Research
- Nutrition, Genetics, and Disease
- Glycosylation and Glycoproteins Research
- Viral Infections and Outbreaks Research
- interferon and immune responses
- Genomics and Chromatin Dynamics
- Toxin Mechanisms and Immunotoxins
- Viral Infections and Vectors
- Plant Virus Research Studies
- Bioinformatics and Genomic Networks
- CAR-T cell therapy research
- Endoplasmic Reticulum Stress and Disease
- Bacterial Genetics and Biotechnology
- Cancer-related Molecular Pathways
- Protein Kinase Regulation and GTPase Signaling
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Microbial Metabolic Engineering and Bioproduction
Novo Nordisk Foundation
2016-2024
University of Copenhagen
2016-2024
Rigshospitalet
2013
Universidad de Costa Rica
2013
Article13 May 2020Open Access Source DataTransparent process Mechanisms of site-specific dephosphorylation and kinase opposition imposed by PP2A regulatory subunits Thomas Kruse orcid.org/0000-0002-2619-7388 Novo Nordisk Foundation Center for Protein Research, Faculty Health Medical Sciences, University Copenhagen, Denmark Search more papers this author Sebastian Peter Gnosa Biotech Research Innovation Centre (BRIC), Isha Nasa Biochemistry Cell Biology, Geisel School Medicine at Dartmouth...
Article13 June 2022Open Access Source DataTransparent process Chemogenetic profiling reveals PP2A-independent cytotoxicity of proposed PP2A activators iHAP1 and DT-061 Gianmatteo Vit orcid.org/0000-0002-3111-0769 Novo Nordisk Foundation Center for Protein Research, Faculty Health Medical Sciences, University Copenhagen, Denmark Contribution: Conceptualization, Formal analysis, Supervision, Funding acquisition, Validation, Investigation, Visualization, Methodology, Writing - original draft,...
The kinase CDK1 directly and indirectly inhibits PP2A holoenzyme formation to promote mitosis.
Abstract SUMOylation regulates numerous cellular processes, but what represents the essential functions of this protein modification remains unclear. To address this, we performed genome-scale CRISPR–Cas9-based screens, revealing that BLM-TOP3A-RMI1-RMI2 (BTRR)-PICH pathway, which resolves ultrafine anaphase DNA bridges (UFBs) arising from catenated structures, and poorly characterized NIP45/NFATC2IP become indispensable for cell proliferation when is inhibited. We demonstrate NIP45...
Several members of the Ly-6/uPAR (LU)-protein domain family are differentially expressed in human squamous epithelia. In some cases, they even play important roles maintaining skin homeostasis, as exemplified by secreted single member, SLURP-1, deficiency which is associated with development palmoplantar hyperkeratosis congenital disorder Mal de Meleda. present study, we have characterized a new member LU-protein family, find to be predominantly stratum granulosum skin, thus resembling...
Short linear motifs (SLiMs) are the most ubiquitous protein interaction modules in unstructured regions of human proteome. Despite their central role function, our understanding contribution SLiMs to cellular homeostasis remains limited. To address this, we designed base editor libraries precisely mutate all curated and a set computationally predicted instances defined by SLiM-like evolutionary patterns. By targeting 7,293 SLiM containing with 80,473 mutations, define dependency map...
Report11 May 2021Open Access Source DataTransparent process A highly conserved pocket on PP2A-B56 is required for hSgo1 binding and cohesion protection during mitosis Yumi Ueki orcid.org/0000-0003-2898-3267 The Novo Nordisk Foundation Center Protein Research, Faculty of Health Medical Science, University Copenhagen, Denmark Search more papers by this author Michael Hadders orcid.org/0000-0002-2653-2625 Oncode Institute Molecular Medicine, Utrech, Utrecht University, Utrecht, Netherlands...
Abstract Translesion DNA synthesis (TLS) is an essential process that allows cells to bypass lesions encountered during replication and emerging as a primary target of chemotherapy. Among vertebrate polymerases, polymerase kappa (Pol() has the unique ability minor groove adducts in vitro. However, Pol(is also required for overcome major but basis this requirement unclear. Here, we combine CRISPR base editor screening technology human with TLS analysis defined Xenopus egg extracts unravel...
Abstract PP2A is an abundant phosphoprotein phosphatase that acts as a tumor suppressor. For this reason, compounds able to activate are attractive anticancer agents. The small molecule iHAP1 and DT-061 have recently been reported by Leonard et al. (2020) Morita in Cell selectively stabilize specific PP2A-B56 complexes which mediate cell killing. Here, we show not the case question key findings these papers. Through genome wide CRISPR-Cas9 screens, uncover biological pathways targeted...