A5053954855- Peptidase Inhibition and Analysis
- Cancer Mechanisms and Therapy
- Mechanisms of cancer metastasis
- Cancer, Hypoxia, and Metabolism
- HER2/EGFR in Cancer Research
- Cell Adhesion Molecules Research
- FOXO transcription factor regulation
- Signaling Pathways in Disease
- Nuclear Physics and Applications
- ATP Synthase and ATPases Research
- Radiopharmaceutical Chemistry and Applications
- Advanced Radiotherapy Techniques
- Cancer-related Molecular Pathways
- Protease and Inhibitor Mechanisms
- Metabolism, Diabetes, and Cancer
- Bioinformatics and Genomic Networks
- Prostate Cancer Treatment and Research
- Viral Infectious Diseases and Gene Expression in Insects
- CRISPR and Genetic Engineering
- Lung Cancer Treatments and Mutations
- Cancer Treatment and Pharmacology
- bioluminescence and chemiluminescence research
- Advanced Proteomics Techniques and Applications
- Ubiquitin and proteasome pathways
- Phagocytosis and Immune Regulation
University of Copenhagen
2019-2024
Linköping University
2011-2019
Örebro County Council
2014
Article13 May 2020Open Access Source DataTransparent process Mechanisms of site-specific dephosphorylation and kinase opposition imposed by PP2A regulatory subunits Thomas Kruse orcid.org/0000-0002-2619-7388 Novo Nordisk Foundation Center for Protein Research, Faculty Health Medical Sciences, University Copenhagen, Denmark Search more papers this author Sebastian Peter Gnosa Biotech Research Innovation Centre (BRIC), Isha Nasa Biochemistry Cell Biology, Geisel School Medicine at Dartmouth...
Abstract Background Astrocyte elevated gene 1 (AEG-1), an important oncogene, has been shown to be overexpressed in several types of cancers. In colorectal cancer (CRC), the protein level AEG-1 is up-regulated tumour tissue compared normal mucosa, showing prognostic significance. Since little known about transcriptional expression and its biological pathway CRC aim present study was examine relationship mRNA expression, clinicopathological variables as well biology CRC. Material methods The...
Dicer is aberrantly expressed in several types of cancers. Applying real-time PCR, we detected the expression mRNA normal mucosa (n = 162), primary colorectal cancer (CRC) 162) and liver metastasis 37), analysed relationship between clinicopathological features. We also correlated to miRNA miR-141, miR-200a, miR-200b, mir-200c miR-429 metastases.RT-PCR qPCR were used analyse mucosa, tumour by using High Capacity cDNA Reverse Transcription Kit TaqMan™® Gene Expression assays for GAPDH. RT-PCR...
Abstract Pancreatic ductal adenocarcinoma (PDAC) is an extremely aggressive malignancy with minimal treatment options and a global rise in prevalence. PDAC characterized by frequent driver mutations including KRAS TP53 (p53) , dense, acidic tumor microenvironment (TME). The relation between genotype TME development unknown. Strikingly, when wild type (WT) Panc02 cells were adapted to growth returned normal pH mimic invasive escaping regions, they displayed strong increase of traits such as...
Macrophages in the tumor microenvironment have a substantial impact on progression. Depending signaling environment tumor, macrophages can either support or constrain It is therefore of therapeutic interest to identify tumor-derived factors that control macrophage education. With this aim, we correlated expression A Disintegrin and Metalloproteinase (ADAM) proteases, which are key mediators cell-cell signaling, protumorigenic markers human cancer cohorts. We identified ADAM17, sheddase...
Preoperative radiotherapy (RT) is widely used to downstage rectal tumours, but the rate of recurrence varies significantly. Therefore, new biomarkers are needed for better treatment and prognosis. It has been shown that astrocyte elevated gene-1 (AEG-1) a key mediator migration, invasion, resistance. Our aim was analyse AEG-1 expression in relation RT cancer patients test its radiosensitising properties.The examined by immunohistochemistry 158 from Swedish clinical trial RT. Furthermore, we...
// Sebastian Gnosa 1 , Alessandra Capodanno Raghavendra Vasudeva Murthy Lasse Dahl Ejby Jensen 2 Xiao-Feng Sun Department of Oncology and Clinical Experimental Medicine, Linköping University, Linköping, Sweden Division Cardiovascular Medical Health Sciences, Correspondence to: Gnosa, email: gnosasebastian@gmail.com Sun, xiao-feng.sun@liu.se Keywords: MTDH, colon cancer, zebrafish, transwell migration invasion, radiation Received: August 15, 2016 Accepted: October 13, Published: November 07,...
The transmembrane glycoprotein basigin, a member of the immunoglobulin superfamily, stimulates matrix metalloproteinase (MMP)-mediated extracellular (ECM) degradation and thereby drives cancer cell invasion. Basigin is proteolytically shed from surface high concentrations soluble basigin in blood dictates poor prognosis patients. A positive correlation between disintegrin (ADAM)-12 serum prostate patients has been reported. Yet, functional relevance this unknown. Here, we show that ADAM12...
Background Tafazzin (TAZ), a transmembrane protein contributes in mitochondrial structural and functional modifications through cardiolipin remodeling. TAZ mutations are associated with several diseases, but studies on the role of carcinogenesis radiotherapy (RT) response is lacking. Therefore we investigated expression rectal cancer, its correlation RT, clinicopathological biological variables patients participating clinical trial preoperative RT. Methods 140 cancer were included this...
Radiotherapy is one of the most common cancer treatments, yet, some patients require high doses to respond. Therefore, development new strategies leans toward personalizing therapy avoid unnecessary burden on patients. This approach prevents administration ineffective treatments or uses combination increase sensitivity cells. ADAM12 has been shown be upregulated in many cancers and correlate with poor survival chemoresistance, thus making it a potential candidate responsible for...
Desmoplasia is a common feature of aggressive cancers, driven by complex interplay protein production and degradation. Basigin type 1 integral membrane receptor secreted in exosomes or released ectodomain shedding from the cell surface. Given that soluble basigin increased circulation patients with poor cancer prognosis, we explored putative role ADAM12-generated progression. We show recombinant binds β1 integrin stimulates gelatin degradation migration cells matrix metalloproteinase (MMP)-...
Abstract The colorectal carcinogenesis is a complex process encompassing genetic alterations. oncoprotein AEG-1, encoded by the MTDH gene, was shown previously to be involved in cancer (CRC). aim of this study determine frequency and spectrum variants tumor tissue, their relationship clinicopathological variables CRC patients. included tumors from 356 unselected Mutation analysis including coding region adjacent intronic sequences, performed direct DNA sequencing. corresponding normal tissue...
Background Peroxisome proliferator-activated receptor delta (PPARD) is nuclear hormone involved in colorectal cancer (CRC) differentiation and progression. The purpose of this study was to determine prevalence spectrum variants the PPARD gene CRC, their contribution clinicopathological endpoints. Methods Findings Direct sequencing performed 303 primary tumors, blood samples from 50 patients with ≥3 affected first-degree relatives, 2 sporadic patients, 360 healthy controls, 6 colon cell...
Abstract Prostate cancer is the second most common diagnosis worldwide and fifth leading cause of related death among men. Several treatment strategies are used including FDA approved 177Lu-vipivotide tetraxetan (177Lu-PSMA-617) for prostate-specific membrane antigen (PSMA)-positive metastatic castration-resistant prostate cancer. However, not all patients respond to due tumor intrinsic resistance mechanisms. Therefore, combination targeted radionuclide therapy with inhibitors against e.g....
Abstract Despite much effort invested in developing novel cancer therapies, more than 90% of clinical studies fail due to toxicity or lack efficacy patients. This can mainly be ascribed relevant and translational preclinical models. A common model used is the xenograft using immortalized cells. Cell line-based models are cost efficient genetically manipulated but often criticized for lacking value. murine with higher relevance patient derived xenografts (PDX) established by implanting...
Tumor progression and response to treatment are highly affected by interactions between cancer cells the tumor microenvironment (TME). Many of soluble factors signaling receptors involved in this crosstalk shed a disintegrin metalloproteinases (ADAMs). Upregulation ADAM15 has been linked worse survival patients tumor-promoting function both vitro murine models. Although cell-cell cell-extracellular matrix interactions, its role TME vivo remains unexplored. Therefore, we aimed understand how...
Abstract Background: Colorectal cancer (CRC) is the third most common in men and second woman worldwide. The therapy of CRC has been critically improved during past two decades, but treatment response varies significantly between different treatments patients. Therefore it need to search for biomarkers more suitable prognosis treatment. aim this study was investigate genetic variants astrocyte elevated gene-1 (AEG-1), its expression patient samples colon cell lines potential correlation...
Abstract Prostate cancer is the second most common diagnosis worldwide and fifth leading cause of related death among men. Lutetium Lu 177 vivivotide tetraxetetan was recently approved by FDA for treatment prostate-specific membrane antigen (PSMA)-positive metastatic castration-resistant prostate cancer. However, not all patients respond to combination therapies may improve outcomes. One approach combine targeted radionuclide therapy with inhibitors targeting DNA damage response pathways...