A5030192348- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Genomics and Chromatin Dynamics
- Cellular transport and secretion
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- RNA modifications and cancer
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Chromosomal and Genetic Variations
- Crystallization and Solubility Studies
- Protein Kinase Regulation and GTPase Signaling
- X-ray Diffraction in Crystallography
- Plant nutrient uptake and metabolism
- Enzyme Structure and Function
- Cancer-related Molecular Pathways
- Monoclonal and Polyclonal Antibodies Research
- Nuclear Structure and Function
- Neuroscience and Neuropharmacology Research
- interferon and immune responses
- Glycosylation and Glycoproteins Research
- Phenothiazines and Benzothiazines Synthesis and Activities
- Biochemical and Molecular Research
- Conducting polymers and applications
University of Copenhagen
2016-2025
Novo Nordisk Foundation
2015-2024
Foundation Center
2024
Lund University
1997-2020
Thinfilm (Sweden)
2012
Aarhus University
1998-2008
Chemical Synthesis Lab
2008
The Gurdon Institute
2008
AstraZeneca (Sweden)
2007
NeuroSearch (Denmark)
2006
BubR1 is a central component of the spindle assembly checkpoint that inhibits progression into anaphase in response to improper kinetochore-microtubule interactions. In addition, also helps stabilize interactions by counteracting Aurora B kinase but mechanism behind this not clear. Here we show directly binds B56 family protein phosphatase 2A (PP2A) regulatory subunits through conserved motif phosphorylated cyclin-dependent 1 (Cdk1) and polo-like (Plk1). Two highly hydrophobic residues...
Nuclear export of intron-containing human immunodeficiency virus type 1 (HIV-1) RNA is mediated by the viral Rev protein that contains both an binding domain specific for response element (RRE) and a nuclear signal (NES). The cellular CRM1 (Exportin1) functions as receptor proteins carrying Rev-like NES in process also requires GTP bound form Ran GTPase. Using purified recombinant factors, we show co-precipitation, gel mobility shift footprinting assays full-length interacts directly with...
Scalable circuits of organic logic and memory are realized using all-additive printing processes. A 3-bit complementary decoder is fabricated used to read write non-volatile, rewritable ferroelectric memory. The decoder-memory array patterned by inkjet gravure on flexible plastics. Simulation models for the transistors developed, enabling circuit designs tolerant variations in printed devices. We explain key design rules fabrication complex elucidate performance requirements materials...
Nonproteolytic ubiquitylation of chromatin surrounding deoxyribonucleic acid (DNA) double-strand breaks (DSBs) by the RNF8/RNF168/HERC2 ubiquitin ligases facilitates restoration genome integrity licensing to concentrate caretaker proteins near lesions. In parallel, SUMOylation so-far elusive upstream DSB regulators is also required for execution this ubiquitin-dependent response. We show that HERC2 and RNF168 are novel DNA damage–dependent targets in human cells. response DSBs, both were...
The Bub1-Bub3 and BubR1-Bub3 checkpoint complexes, or the Bubs, contribute to accurate segregation of chromosomes during mitosis by promoting chromosome bi-orientation halting exit from if this fails. complexes associate with kinetochores mitosis, which is required for proper segregation. outer kinetochore protein KNL1 (also known as CASC5/Blinkin/AF15Q14) receptor Bub proteins but exact nature functional binding sites on are yet be determined. Here, we show that contains multiple proteins,...
Abstract The spindle assembly checkpoint (SAC) ensures proper chromosome segregation by delaying anaphase onset in response to unattached kinetochores. Checkpoint signalling requires the kinetochore localization of Mad1–Mad2 complex that more eukaryotes depends on Rod–Zwilch–ZW10 (RZZ) complex. protein Zwint has been proposed be receptor for RZZ, but here we show Bub1 and not is required RZZ recruitment. We find middle region encompassing a domain essential SAC contributes localization. In...
Article13 May 2020Open Access Source DataTransparent process Mechanisms of site-specific dephosphorylation and kinase opposition imposed by PP2A regulatory subunits Thomas Kruse orcid.org/0000-0002-2619-7388 Novo Nordisk Foundation Center for Protein Research, Faculty Health Medical Sciences, University Copenhagen, Denmark Search more papers this author Sebastian Peter Gnosa Biotech Research Innovation Centre (BRIC), Isha Nasa Biochemistry Cell Biology, Geisel School Medicine at Dartmouth...
Abstract Proper segregation of chromosomes depends on a functional spindle assembly checkpoint (SAC) and requires kinetochore localization the Bub1 Mad1/Mad2 proteins. Several aspects recruitment in human cells are unclear particular underlying direct interactions. Here we show that conserved domain 1 (CD1) binds directly to Mad1 phosphorylation site exists CD1 stimulates binding SAC signalling. Importantly, fusion minimal kinetochore-targeting fragments bypasses need for CD1, revealing main...
Viral proteins make extensive use of short peptide interaction motifs to hijack cellular host factors. However, most current large-scale methods do not identify this important class protein-protein interactions. Uncovering mediated interactions provides both a molecular understanding viral with their and the foundation for developing novel antiviral reagents. Here we describe discovery approach covering 23 coronavirus strains that high resolution information on direct virus-host We 269...
RNA editing of specific residues by adenosine deamination is a nuclear process catalyzed deaminases acting on (ADAR). Different promoters in the ADAR1 gene give rise to two forms protein: constitutive promoter expresses transcript encoding (c)ADAR1, and an interferon-induced N-terminally extended form, (i)ADAR1. Here we show that (c)ADAR1 primarily whereas (i)ADAR1 encompasses functional export signal N-terminal part nucleocytoplasmic shuttle protein. Mutation or treatment with CRM1-specific...
Genome integrity is critically dependent on timely DNA replication and accurate chromosome segregation. Replication stress delays into G2/M, which in turn impairs proper segregation inflicts damage the daughter cells. Here we show that TopBP1 forms foci upon mitotic entry. In early mitosis, marks sites of promotes unscheduled synthesis. Moreover, required for focus formation structure-selective nuclease scaffold protein SLX4 mitosis. Persistent transition 53BP1 nuclear bodies (NBs) G1...
The recruitment of substrates by the ser/thr protein phosphatase 2A (PP2A) is poorly understood, limiting our understanding PP2A-regulated signaling. Recently, first PP2A:B56 consensus binding motif, LxxIxE, was identified. However, most validated LxxIxE motifs bind with micromolar affinities, suggesting that additional exist to enhance binding. Here, we report requirement a positively charged motif in subset interactors, including KIF4A, facilitate B56 via dynamic, electrostatic...