A5082252396- Enzyme-mediated dye degradation
- Viral Infectious Diseases and Gene Expression in Insects
- Protein purification and stability
- Cancer Research and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Enzyme Catalysis and Immobilization
- Protein Interaction Studies and Fluorescence Analysis
- Microbial Metabolism and Applications
- Chromium effects and bioremediation
- RNA Interference and Gene Delivery
- Bacterial Genetics and Biotechnology
- Photosynthetic Processes and Mechanisms
- Glycosylation and Glycoproteins Research
- Enzyme Structure and Function
- Arsenic contamination and mitigation
- Polyamine Metabolism and Applications
- Click Chemistry and Applications
- Advanced biosensing and bioanalysis techniques
- Electrochemical sensors and biosensors
- RNA and protein synthesis mechanisms
- Virus-based gene therapy research
- Steroid Chemistry and Biochemistry
- Bacteriophages and microbial interactions
- Crystallization and Solubility Studies
TU Wien
2018-2025
Glycosylation is the most prevalent protein post‐translational modification, with a quarter of glycosylated proteins having enzymatic properties. Yet, full impact glycosylation on structure–function relationship, especially in enzymes, still limited. Here, we show that rigidifies important commercial enzyme horseradish peroxidase (HRP), which turn increases its turnover and stability. Circular dichroism spectroscopy revealed increased holo‐HRP's thermal stability promoted significant helical...
Horseradish peroxidase (HRP) is an intensely studied enzyme with a wide range of commercial applications. Traditionally, HRP extracted from plant; however, recombinant (rHRP) production promising alternative. Here, non-glycosylated rHRP was produced in Escherichia coli as DsbA fusion protein including Dsb signal sequence for translocation to the periplasm and His tag purification. The missing N-glycosylation results reduced catalytic activity thermal stability, therefore engineering used...
Biopharmaceutical drug substances are generally produced using fermentation technology and subsequently purified in the following downstream process. For determination of critical quality attributes (CQAs), such as target protein titer purity, monitoring tools required before control analysis. We herein present a novel reversed phase liquid chromatography method (RPLC), which enables facile robust quantification during upstream processing intracellularly proteins E. coli. The overall goal...
Horseradish peroxidase (HRP), an enzyme omnipresent in biotechnology, is still produced from hairy root cultures, although this procedure time-consuming and only gives low yields. In addition, the plant-derived preparation consists of a variable mixture isoenzymes with high batch-to-batch variation preventing its use therapeutic applications. study, we present novel scalable recombinant HRP production process Escherichia coli that yields highly pure, active homogeneous single isoenzyme. We...
Fighting cancer still relies on chemo- and radiation therapy, which is a trade-off between effective clearance of malignant cells severe side effects healthy tissue. Targeted treatment the other hand promising refined strategy with less systemic interference. The enzyme horseradish peroxidase (HRP) exhibits cytotoxic in combination indole-3-acetic acid (IAA). However, plant-derived out bounds for medical purposes due to its foreign glycosylation pattern resulting rapid immunogenicity. In...
Refolding is known as the bottleneck in inclusion body (IB) downstream processing pharmaceutical industry: high dilutions leading to large operating volumes, slow refolding kinetics and low yields are only a few of problems that impede industrial application. Solubilization prior often carried out empirically effects solubilizate on subsequent step rarely investigated. The results obtained this study, however, indicate quality IB has severe effect refolding. As contains chaotropic reagents...
Targeted cancer treatment is a promising, less invasive alternative to chemotherapy as it precisely directed against tumor cells whilst leaving healthy tissue unaffected. The plant-derived enzyme horseradish peroxidase (HRP) can be used for prodrug therapy with indole-3-acetic acid or the analgesic paracetamol (acetaminophen). Oxidation of by HRP in presence hydrogen peroxide leads
Targeted cancer therapy is a promising alternative to the currently established treatments, aiming selectively kill cells while sparing healthy tissues. Hereby, molecular targeting agents, such as monoclonal antibodies, are used bind cell surface markers specifically. Although these agents have shown great clinical success, limitations still remain low tumor penetration and off-target effects. To overcome this limitation, novel fusion proteins comprised of two ADAPT6 Horseradish Peroxidase...
ABSTRACT Glycosylation is the most prevalent protein post-translational modification, with a quarter of glycosylated proteins having enzymatic properties. Yet full impact glycosylation on structure-function relationship, especially in enzymes, still limited. Here we show rigidifies important commercial enzyme horseradish peroxidase (HRP), which turn increases its activity and stability. Circular dichroism spectroscopy revealed that increased holo-HRP’s thermal stability promoted significant...
Abstract The enzyme Horseradish Peroxidase (HRP) is omnipresent in modern biotechnology. Although promising for therapeutic purposes, no suitable production process this has been available until now. Medical applications require the to be highly pure, homogenous and well-defined. We have developed an efficient recombinant HRP from Escherichia coli inclusion bodies. With strategy we are able provide active, pure non-glycosylated at competitive yields.