A5041191271- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Vector-borne infectious diseases
- Viral Infections and Vectors
- Toxin Mechanisms and Immunotoxins
- Leptospirosis research and findings
- Bacterial Identification and Susceptibility Testing
- Vector-Borne Animal Diseases
- Viral gastroenteritis research and epidemiology
- Microbial infections and disease research
- Genetic and phenotypic traits in livestock
- Autoimmune and Inflammatory Disorders Research
- Antimicrobial Resistance in Staphylococcus
- Veterinary Medicine and Surgery
- Antibiotic Use and Resistance
- Veterinary Equine Medical Research
- Insect and Pesticide Research
- Parasitic Diseases Research and Treatment
- Bartonella species infections research
- Rabies epidemiology and control
- Cardiovascular Conditions and Treatments
- Parasites and Host Interactions
- Blood Coagulation and Thrombosis Mechanisms
- Eosinophilic Disorders and Syndromes
- Agriculture, Plant Science, Crop Management
University of Naples Federico II
2011-2024
Institut Pasteur de Tunis
2024
Federico II University Hospital
2006-2014
ABSTRACT Most experience in the comparison of diagnostic tools for canine leishmaniasis comes from cross-sectional surveys dogs different ages and breeds cases with unknown onset duration leishmaniasis. A longitudinal study was performed on 43 beagle exposed to three transmission seasons (2002 2004) Mediterranean examined periodically over 32 months through bone marrow microscopy nested PCR (n-PCR), lymph node culture, serology (immunofluorescent-antibody test), evaluation clinical...
Canine leishmaniasis is an important zoonosis caused by uncontrolled infection with Leishmania infantum, where inappropriate immune response not only responsible for permitting this intracellular parasite to multiply, but also several of the pathological processes seen in disease. Effective canine vaccines are therefore a highly desirable prevention tool. In randomised, double-blinded, controlled trial, efficacy LiESP/QA-21 vaccine (CaniLeish, Virbac, France) was assessed exposing 90 naïve...
The incidence of clinical and clinicopathological signs associated with the progression infection was evaluated prospectively in 329 naïve young dogs exposed to Leishmania infantum transmission examined periodically during 22 months (M). were part vaccine investigations performed under natural conditions. Vaccinated groups considered evaluation when resulted non-protective appearance did not differ statistically from controls at each time point, otherwise only control included. 115 beagles 3...
Ten Beagle dogs at different stages of Leishmania infantum infection, among which 6 had received a full course LiESP/QA-21 (CaniLeish(®); Virbac) vaccination, were exposed to the bites reared Phlebotomus perniciosus assesses their infectiousness potential. This was found be negligible/nil in 2 seronegative with subpatent infection. Among 8 active infection (=positive serology, bone-marrow qualitative PCR and lymph node culture), 2/5 vaccinated (40.0%) 2/3 nonvaccinated (66.7%) infectious...
Canine leishmaniosis (CanL) caused by Leishmania infantum is a widespread endemic disease in SW Europe. This study was designed to determine how veterinarians clinically manage CanL this region analysing information collected questionnaire completed local working clinics France, Portugal, Greece, Spain, Italy and Slovenia. Over the period 2004–2011, on sent 12,546 small animal located six countries surveyed. The with 10 items comprising open closed questions sought obtain comparable data...
Bone marrow aspiration smears from 15 dogs naturally infected with leishmania were evaluated. Three of the showed no clinical signs, six had up to three signs and more than three. The most common pathological features bone megakaryocytic dysplasia in 10 dogs, erythrophagocytosis eight, erythroid two emperipolesis two. probably related an increased number macrophages producing high levels tumour necrosis factor alpha interferon gamma. Six dysplastic erythrophagocytosis, suggesting that...
Canine vector-borne diseases (CVBDs) are a spectrum of caused by different pathogens transmitted blood-feeding arthropoda. The aim this study was to investigate leishmaniosis, babesiosis and filarial infections in dogs with three lifestyles (hunting, stray sheep dogs) Molise, the small region southern Italy, where data available about these parasitic very scant. A cross-sectional survey conducted on 318 hunting, 180 218 dogs. Immunofluorescence antibody test, blood smear, molecular...
Canine leishmaniosis (CanL) is caused by protozoans of the genus Leishmania and characterized a broad spectrum clinical signs in dogs. Early diagnosis great importance order to perform an appropriate therapy prevent progression towards severe disease. The aim this study was compare point-of-care molecular technique, i.e., loop-mediated isothermal amplification (LAMP), with real-time polymerase chain reaction (Rt-PCR), three serological techniques, immunofluorescence antibody test (IFAT),...
Canine leishmaniosis (CanL) is an emerging zoonosis caused by Leishmania infantum and transmitted in southern Europe phlebotomine sand flies of the subgenus Phlebotomus (Larroussius). Endemic foci CanL have been recorded northern continental Italy since early 1990s attributed to northward expansion vector populations due climatic changes association with travelling/relocated infected dogs from Mediterranean littoral. In this study, further spread endemic was monitored during 2018-2019 five...
An 8-year-old intact male mongrel dog with alopecia and weight loss was referred to the Veterinary Faculty of Naples. The had pale mucous membranes, enlarged prescapular lymph nodes, splenomegaly. Laboratory abnormalities included anemia, thrombocytopenia, hyperglobulinemia. Bone marrow aspirate smears contained numerous Leishmania amastigotes an immunofluorescent antibody titer strongly positive (1:1280) for leishmaniasis. treated a combination meglumine antimoniate allopurinol 60 days...
Abstract Canine leishmaniosis is a severe systemic disease caused by the kinetoplastid protozoan Leishmania infantum , an obligatory intracellular parasite of mammalian macrophages, transmitted bite phlebotomine sandflies. The infection in dogs might occur without any clinical signs or be characterised chronic viscerocutaneous signs, such as lymphadenopathy, skin lesions, splenomegaly, onychogryphosis, and renal well ocular damage due to immunocomplex deposition. In atypical cases parasites...
Bone-marrow aspirate material is commonly considered as one of the most sensitive tissues for a reliable diagnosis leishmaniasis. The procedure herein described may permit less experienced veterinarians to be familiar with quick and safe assessment method leishmaniasis in their patients. Animals are positioned right lateral recumbency, area corresponding second, third, or fourth sternebra identified aseptically prepared. A 18-gauge needle connected 10 mL syringe driven through skin, up bone...
During last decade Lampedusa island (Italy) has been interested by a deep social change caused the massive arrival of migrants from north Africa. The goal this study was to evaluate current CanL burden and risk factors for Visceral Leishmaniosis (VL) on Lampedusa, actually based very few data obtained in previous performed fifteen years ago. Two hundred forty-two dogs were enrolled detection Leishmania infantum infection serology. In addition, an entomological investigation confirm presence...
Abstract Background Inflammatory myopathy and perivasculitis have been recently described in horses with chronic equine piroplasmosis (EP). These alterations may be linked to poor performances. The aims of this study were evaluate the prevalence for EP clinically healthy Italian Standardbred (IS) racehorses compare laboratory parameters performance metrics between positive negative horses. Real-time PCR was applied detection T. equi B. caballi positivity. Haematology parameters, blood...
Abstract Background The aim of this study was to determine exposure vector-borne pathogens (VBPs) in populations dogs living on Greek islands the Ionian and Aegean seas. Methods In total, 1154 with different lifestyles varying ages breeds were randomly sampled examined for presence clinical signs compatible canine diseases (CVBDs). Blood collected from each individual animal. For detection antibodies against Leishmania spp., WITNESS® test performed, positive samples further indirect...
Canine leishmaniasis (CanL) is a disease characterized by large variety of clinical alterations, the majority which being due to immune mediated mechanisms. Sick dogs usually produce high levels Leishmania-specific immunoglobulins may give rise circulating complexes (CICs) whose defective clearance scavenging macrophages induces vasculitis and their deposition in specific organs. The aim this study was assess serum level CICs exposed natural experimental infection. Fifty-two sera were...