A5030101015- Ion Channels and Receptors
- Neurobiology and Insect Physiology Research
- Dermatology and Skin Diseases
- Herbal Medicine Research Studies
- Ion channel regulation and function
- Pain Mechanisms and Treatments
- MicroRNA in disease regulation
- Neuroendocrine regulation and behavior
- Biochemical Analysis and Sensing Techniques
- Bioactive Compounds and Antitumor Agents
- Stress Responses and Cortisol
- Phytochemicals and Antioxidant Activities
- Postharvest Quality and Shelf Life Management
- Ion Transport and Channel Regulation
- Menstrual Health and Disorders
- Monoclonal and Polyclonal Antibodies Research
- Neural dynamics and brain function
- Hepatitis C virus research
- Plant Reproductive Biology
- Neuroscience and Neural Engineering
- Food Allergy and Anaphylaxis Research
- EEG and Brain-Computer Interfaces
- Nitric Oxide and Endothelin Effects
- Urticaria and Related Conditions
- Circadian rhythm and melatonin
University of Bucharest
2013-2024
Friedrich-Alexander-Universität Erlangen-Nürnberg
2023
Romanian Academy
2017
Institute of Biochemistry
2017
University College Cork
2013
We demonstrate a novel dual strategy against inflammation and pain through body-wide desensitization of nociceptors via TRPA1. Attenuation experimental colitis by capsazepine (CPZ) has long been attributed to its antagonistic action on TRPV1 associated inhibition neurogenic inflammation. In contrast, we found that CPZ exerts anti-inflammatory effects profound Micromolar induced calcium influx in isolated dorsal root ganglion (DRG) neurons from wild-type (WT) but not TRPA1-deficient mice....
Camphor is known to potentiate both heat and cold sensations. Although the sensitization could be explained by activation of heat-sensitive transient receptor potential (TRP) channels TRPV1 TRPV3, camphor-induced cooling remains unexplained. In this study, we present evidence for cold- menthol-sensitive channel melastatin 8 (TRPM8) camphor. Calcium transients evoked camphor in HEK293 cells expressing human rat TRPM8 are inhibited antagonists...
Abstract The transient receptor potential ankyrin type 1 (TRPA1) channel belongs to the TRP superfamily of ion channels. TRPA1 is a membrane protein with multiple functions able respond noxious stimuli, reactive oxygen species, inflammatory cytokines or pungent substances, and it participates in pain signalling, taste, inflammation various steps tumorigenic process. To date, no reports have addressed expression function pancreatic ductal adenocarcinoma (PDAC) cells. This work endogenous...
Abstract The transient receptor potential melastatin type 8 (TRPM8) channel is expressed in primary afferent neurons where it the main transducer of innocuous cold temperatures and also a variety tumors, involved progression metastasis. Modulation this by intracellular signaling pathways has therefore important clinical implications. We investigated modulation recombinant natively TRPM8 Src kinase, which known to be cancer pathophysiology inflammation. Human HEK293T cells constitutively...
Lay Summary Managing itching and the effects of histamine can be difficult for people with allergies. Cooling skin or applying menthol provides some relief from itch, but way they work is not fully understood. agents interact a protein called TRPM8 (also known as ‘cold receptor’) have shown potential management itch. However, much research has been done on animals limitations when compared human studies. Antihistamine medications help histamine-induced itching, may other causes This study...
Abstract Serotonin (5‐hydroxytryptamine, 5‐HT) released by platelets, mast cells, and immunocytes is a potent inflammatory mediator which modulates pain itch sensing in the peripheral nervous system. The serotonergic receptors expressed primary afferent neurons involved these sensory functions are not fully identified appear to be large extent species dependent. Moreover, mechanisms through 5‐HT receptor activation coupled changes neuronal excitability have been completely revealed. Using...
Abstract Loss-of-function mutations in the enzyme 7-dehydrocholesterol reductase are responsible for Smith–Lemli–Opitz syndrome, which (7-DHC) levels markedly increased plasma and tissues of patients. This increase 7-DHC is probably associated with painful itchy photosensitivity reported by majority patients syndrome. To identify molecular targets involved activation photosensitization primary afferents 7-DHC, we focused on TRPA1 TRPV1, two ion channels expressed nociceptive nerve endings...
The TREK family of leak potassium channels has been found to play critical roles in nociception, sensitivity general anaesthetics, neuroprotection, and memory. three members the family, TREK1, TREK2 TRAAK establish resting potential modify duration, frequency amplitude action potentials. Despite their apparent importance, repertoire regulatory interactions utilized by cells control expression is poorly understood. Herein, contribution miRNAs regulation post-transcriptional gene examined....
PUVA (psoralen UVA) therapy is used to treat a variety of skin conditions, such as vitiligo psoriasis, eczema and mycosis fungoides, but it frequently accompanied by phototoxicity leading burning pain, itch erythema.We combination calcium reactive oxygen species (ROS) imaging, patch clamp neuropeptide release measurement investigate whether certain ion channels involved in pain signalling could be responsible for these adverese effects PUVA.Clinically psoralen derivatives 8-methoxypsoralen...
Recent progress in patterned microelectrode manufacturing technology and microfluidics has opened the way to a large variety of cellular molecular biosensor-based applications. In this extremely diverse rapidly expanding landscape, silicon-based technologies occupy special position, given their statute mature, consolidated, highly accessible areas development. Within present work we report microfabrication procedures workflows for 3D gold-plated arrays (MEA) different shapes (pyramidal,...
Methylglyoxal (MG) is a reactive dicarbonyl compound involved in protein modifications linked to diabetes mellitus. The plasma level of MG elevated diabetic patients, particularly those with painful neuropathy. Diabetic neuropathy often associated spontaneous pain and altered thermal perception. This study assesses effects on TRPM8, an ion channel innocuous cold sensing allodynia also cold-mediated analgesia. Acute treatment inhibited the activation recombinant rat human transient receptor...
Abstract Removing water from wet fur or feathers is important for thermoregulation in warm-blooded animals. The “wet dog shake” (WDS) behavior has been largely characterized mammals but to a much lesser extent birds. Although it known that TRPM8 the main molecular transducer of low temperature mammals, not clear if wetness-induced shaking furred and feathered animals dependent on TRPM8. Here, we show novel agonist induces WDS rodents and, importantly, birds, similar evoked by water-spraying....
An amendment to this paper has been published and can be accessed via a link at the top of paper.
The TRPM8 ion channel is involved in innocuous cold sensing and has a potent anti-inflammatory action. Its activation by lower temperature or chemical agonists such as menthol icilin induces analgesic effects, reversing hypersensitivity reducing chronic pain. On the other hand, prostacyclin (PGI