A5056406157- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Genomics and Rare Diseases
- Genomic variations and chromosomal abnormalities
- Botulinum Toxin and Related Neurological Disorders
- Nerve injury and regeneration
- Advanced Fluorescence Microscopy Techniques
- Animal Genetics and Reproduction
- Viral Infectious Diseases and Gene Expression in Insects
- Plant tissue culture and regeneration
- Microbial Metabolic Engineering and Bioproduction
- Autism Spectrum Disorder Research
- Toxin Mechanisms and Immunotoxins
- Genetic Associations and Epidemiology
- Protist diversity and phylogeny
- Neurological disorders and treatments
- Genetics and Neurodevelopmental Disorders
University of California, San Diego
2013-2016
Global Algae Innovations (United States)
2012-2014
The idea of targeted therapy, whereby drug or protein molecules are delivered to specific cells, is a compelling approach treating disease. Immunotoxins one such therapeutic, consisting an antibody domain for binding target cells and toxin that inhibits the proliferation cell. One major hurdle preventing these therapies from reaching market has been lack suitable production platform allows cost-effective highly complex molecules. chloroplast green alga Chlamydomonas reinhardtii shown contain...
Fluorescent proteins (FPs) have become essential tools for a growing number of fields in biology. However, such not been widely adopted use microalgal research. The aim this study was to express and compare six FPs (blue mTagBFP, cyan mCerulean, green CrGFP, yellow Venus, orange tdTomato red mCherry) the popular model microalga Chlamydomonas reinhardtii. To circumvent transgene silencing that often occurs C. reinhardtii, were expressed from nuclear genome as transcriptional fusions with...
Transgenic microalgae have the potential to impact many diverse biotechnological industries including energy, human and animal nutrition, pharmaceuticals, health beauty, specialty chemicals. However, major obstacles sophisticated genetic metabolic engineering in algae been lack of well-characterized transformation vectors direct engineered gene products specific subcellular locations, inability robustly express multiple nuclear-encoded transgenes within a single cell. Here we validate set...
Summary We have produced three antitoxins consisting of the variable domains camelid heavy chain‐only antibodies ( V H ) by expressing genes in chloroplast green algae. These accumulate as soluble proteins capable binding and neutralizing botulinum neurotoxin. Furthermore, they at up to 5% total protein, sufficient expression easily produce these scale from The for different were transformed into Chlamydomonas reinhardtii chloroplasts their products purified algae lysates assayed vitro...
Abstract Genetic studies of Autism Spectrum Disorder (ASD) have established that de novo duplications and deletions contribute to risk. However, ascertainment structural variation (SV) has been restricted by the coarse resolution current approaches. By applying a custom pipeline for SV discovery, genotyping assembly genome sequencing 235 subjects, 71 cases, 26 sibling controls their parents, we present an atlas 1.2 million SVs (5,213/genome), comprising 11 different classes. We demonstrate...