A5026303251- Biochemical and Molecular Research
- Cancer, Hypoxia, and Metabolism
- Cell Adhesion Molecules Research
- Cancer Cells and Metastasis
- Lung Cancer Treatments and Mutations
- Colorectal Cancer Treatments and Studies
- Angiogenesis and VEGF in Cancer
- Cancer Treatment and Pharmacology
- Cancer therapeutics and mechanisms
- Lung Cancer Research Studies
- Protein Kinase Regulation and GTPase Signaling
- Melanoma and MAPK Pathways
- Cancer Immunotherapy and Biomarkers
- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Peptidase Inhibition and Analysis
- DNA Repair Mechanisms
- Proteoglycans and glycosaminoglycans research
- Enzyme Structure and Function
- Erythrocyte Function and Pathophysiology
- 3D Printing in Biomedical Research
- Medical Imaging Techniques and Applications
- Bacterial Genetics and Biotechnology
- Tissue Engineering and Regenerative Medicine
- Cancer Risks and Factors
Fred Hutch Cancer Center
2024-2025
Tufts University
2020-2024
Massachusetts General Hospital
2018-2019
Harvard University
2018
University of California, Santa Barbara
2015
Abstract Purpose: KRAS-mutant lung cancers have been recalcitrant to treatments including those targeting the MAPK pathway. Covalent inhibitors of KRAS p.G12C allele allow for direct and specific inhibition mutant in cancer cells. However, as other targeted therapies, therapeutic potential these can be impaired by intrinsic resistance mechanisms. Therefore, combination strategies are likely needed improve efficacy. Experimental Design: To identify maximally leverage we defined response a...
The extracellular matrix (ECM), a major component of the tumor microenvironment, promotes local invasion to drive metastasis. Here, we describe method study whole-tissue ECM effects from disease states associated with metastasis on cell phenotypes and identify individual proteins signaling pathways that are driving these effects. We show decellularized tumor-bearing obese mammary glands drives TNBC invasion. Proteomics gland led us full-length collagen VI as novel driver whose abundance in...
Abstract Triple-negative breast cancer (TNBC) is the most aggressive and deadly subtype of cancer, accounting for 30,000 cases annually in United States. While there are several clinical trials ongoing to identify new agents treat TNBC, majority patients with TNBC treated anthracycline- or taxane-based chemotherapies neoadjuvant setting, followed by surgical resection adjuvant chemotherapy. many respond well this approach, as 25% will suffer local metastatic recurrence within 5 years....
Abstract Background Metastasis is the leading cause of death in breast cancer patients. For metastasis to occur, tumor cells must invade locally, intravasate, and colonize distant tissues organs, all steps that require cell migration. The majority studies on invasion rely human lines. While it known these have different properties abilities for growth metastasis, vitro morphological, proliferative, migratory, invasive behavior lines their correlation vivo poorly understood. Thus, we sought...
Significance Gram-negative bacteria use contact-dependent growth inhibition (CDI) systems to bind neighboring and deliver diverse nuclease toxins that inhibit target-cell growth. This process requires toxin transport across the outer inner membranes of target reach DNA RNA substrates in cytoplasm. Our data indicate CDI contain a variable domain specifies entry pathway into bacteria. These “translocation domains” exploit specific integral membrane proteins linked domains We also find...
Exquisitely chemosensitive initially, small cell lung cancer (SCLC) exhibits dismal outcomes owing to rapid transition chemoresistance. Elucidating the genetic underpinnings has been challenging limitations with cellular models. As SCLC patient-derived xenograft (PDX) models mimic therapeutic responses, we perform screens in PDX identify drivers of cDNA overexpression MYC , MYCN and MYCL while CRISPR deletion KEAP1 loss as driving Deletion switched a model become chemoresistant resulted...
Single cell technologies have described heterogeneity across tissues, but the spatial distribution and forces that drive single phenotypes not been well defined. Combining RNA protein analytics in pancreatic cancer (PDAC) model systems, we demonstrated role of stromal fibroblasts shaping PDAC towards invasive (EMT) proliferative (PRO) phenotypes. Using highcontent digital imaging situ hybridization 195 tumors, observed these EMT PRO subpopulations 319,626 individual cells. Interestingly,...
Abstract Background Metastasis is the leading cause of death in breast cancer patients. For metastasis to occur, tumor cells must invade locally, intravasate, and colonize distant tissues organs, all steps that require cell migration. The majority studies on invasion rely human lines. While it known these have different properties abilities for growth metastasis, vitro morphological, proliferative, migratory, invasive behavior lines their correlation vivo poorly understood. Thus, we sought...
<h3>Background</h3> Small cell lung cancer (SCLC) is an aggressive malignancy harboring a dismal 5-year survival rate of less than 10%. In 2019, the addition anti-PDL1 antibody to initial platinum-etoposide chemotherapy revolutionized standard care after decades ineffective chemoradiation. Nevertheless, observed increase in patient with immune checkpoint blockade (ICB) remains modest, highlighting need for further improvements immunotherapy this disease. Recently, there has been growing...
Abstract Triple-negative breast cancer (TNBC) is the most aggressive and deadly subtype of cancer, accounting for 30,000 cases annually in US. While there are several clinical trials ongoing to identify new agents treat TNBC, majority TNBC patients treated with anthracycline- or taxane-based chemotherapies neoadjuvant setting, followed by surgical resection adjuvant chemotherapy. many respond well this approach, as 25% will suffer local metastatic recurrence within five years. Understanding...
Abstract The extracellular matrix (ECM) is a major component of the tumor microenvironment that supports cellular growth, promotes local invasion from primary tumor, and contributes to metastatic outgrowth in sites colonization. Obesity systemic disease causes chronic inflammation which can lead ECM deposition ultimately fibrosis adipose tissues such as mammary gland. Overweight breast cancer patients have increased metastasis lung liver, exhibit resistance chemotherapy worse outcomes. We...
<div>Abstract<p>Triple-negative breast cancer (TNBC) is the most aggressive and deadly subtype of cancer, accounting for 30,000 cases annually in United States. While there are several clinical trials ongoing to identify new agents treat TNBC, majority patients with TNBC treated anthracycline- or taxane-based chemotherapies neoadjuvant setting, followed by surgical resection adjuvant chemotherapy. many respond well this approach, as 25% will suffer local metastatic recurrence...
Supplementary Data from Chemotherapy-Induced Collagen IV Drives Cancer Cell Motility through Activation of Src and Focal Adhesion Kinase
Supplementary Data from Chemotherapy-Induced Collagen IV Drives Cancer Cell Motility through Activation of Src and Focal Adhesion Kinase
<p>Supplementary Table 1</p>
<p>Supplementary Data 1</p>
<p>Legends to supplementary table, data and figures</p>
<p>Supplementary Figure 5</p>
<p>Supplementary Figure 2</p>
<p>Supplementary Data 2</p>
<p>Supplementary Figure 6</p>
<p>Supplementary Data 3</p>