A5058132500- Advanced Fluorescence Microscopy Techniques
- Photoacoustic and Ultrasonic Imaging
- Adenosine and Purinergic Signaling
- Force Microscopy Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Neuroscience of respiration and sleep
- Cell Image Analysis Techniques
- Neonatal Respiratory Health Research
- Cell Adhesion Molecules Research
- RNA Interference and Gene Delivery
- Image Processing Techniques and Applications
- Lipid Membrane Structure and Behavior
- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- Spectroscopy and Chemometric Analyses
- DNA and Nucleic Acid Chemistry
- Ion channel regulation and function
- Nanopore and Nanochannel Transport Studies
- Near-Field Optical Microscopy
McGill University
2018-2023
Super-resolution fluorescence imaging based on localization microscopy requires tuning the photoblinking properties of fluorescent dyes employed. Missing is a rapid way to analyze blinking rates fluorophore probes. Herein we present an ensemble autocorrelation technique for rapidly and simultaneously measuring bleaching rate constants from image time series probes that significantly faster than individual single-molecule trajectory analysis approaches. Our method accurate probe densities...
We present a fluorescence fluctuation image correlation analysis method that can rapidly and simultaneously measure the diffusion coefficient, photoblinking rates, fraction of diffusing particles fluorescent molecules in cells. Unlike other techniques, we demonstrated our could be applied irrespective nonuniformly distributed, immobile blinking fluorophore population. This allows us to transport dynamics complex cell morphologies, benefit for range super-resolution imaging approaches rely on...
The mechanisms by which angiotensin II type 1 receptor is distributed and the diffusional pattern in plasma membrane (PM) remain unclear, despite their crucial role cardiovascular homeostasis. In this work, we obtained quantitative information of (AT1R) lateral dynamics as well changes diffusion properties after stimulation with ligands living cells using photoactivated localization microscopy (PALM) combined image spatial-temporal correlation analysis. To study organization at nanoscale,...
1 Abstract The human skeleton constantly interacts and adapts to the physical world. We have previously reported that physiologically-relevant mechanical forces lead small, repairable membrane injuries in bone-forming osteoblasts, resulting release of ATP stimulation purinergic (P2) calcium responses neighbouring cells. goal this study was develop a theoretical model describing injury-related ADP release, extracellular diffusion degradation, neighboring validated using experimental data...
Abstract We present a fluorescence fluctuation image correlation analysis method that can rapidly and simultaneously measure the diffusion coefficient, photoblinking rates, fraction of diffusing particles fluorescent molecules in cells. Unlike other techniques, we demonstrated our could be applied irrespective non-uniformly distributed, immobile blinking fluorophore population. This allows us to transport dynamics complex cell morphologies, benefit for range super-resolution imaging...