SIRT3, a mitochondrial sirtuin belonging to nicotinamide adenine nucleotide (NAD) dependent deacetylases, is implicated in metabolism, longevity and carcinogenesis. SIRT3 expression its significance hepatocellular carcinoma (HCC) remain largely unclear. In this study, we demonstrated that HCC tissue was much lower than paracarcinoma tissue, at both mRNA protein levels. The cutoff value for low defined according receiver operating characteristic curve (ROC) analysis. As disclosed by...

Lung cancer is the one of most frequent causes malignant tumors. In recent years, it has been documented that statins have anticancer and chemopreventive properties. However, mechanism simvastatin on lung still unclear. this study, human cell line A549 cells were incubated with simvastatin. Simvastatin inhibited survival in a dose-dependent manner, decreased Bcl-2 protein expression, increased Bax expression time dose dependently. addition, blocked G1 phase cycle, downregulated cyclin D1...

BackgroundDysregulation of polycomb chromobox (CBX) proteins that mediate epigenetic gene silencing contributes to the progression human cancers. Yet their roles in clear cell renal carcinoma (ccRCC) remain be explored.MethodsThe expression CBX4 and its clinical significance were determined by qRT-PCR, western blot, immunohistochemistry statistical analyses. The biological function ccRCC tumor growth metastasis underlying mechanism investigated using vitro vivo models.FindingsCBX4 exerts...

Cyclin F, capable of forming Skp1-Cul1-F-box protein ubiquitin ligase complex, is implicated in controlling centrosome duplication and preventing genome instability. F oscillates during cell cycle with a similar pattern to cyclin A. However, its expression significance cancer remain obscure. In this study, we showed that was noticeably decreased 16 pairs tissue samples hepatocellular carcinoma (HCC) compared paracarcinoma tissues, at both mRNA levels. Immunohistochemical staining data...

MicroRNAs had been proved to be pivotal regulators in nasopharyngeal carcinoma (NPC) by regulating a large amount of genes' expression. In our research, we aim explore the functions miR-9-3p on metastases NPC and figure out potential mechanisms. First, revealed downregulation upregulation fibronectin 1 (FN1), β1 integrin (ITGB1) α5 (ITGAV) expression tissues cells compared with normal using RNA-seq analysis, RT-qPCR, western blot immunohistochemistry. By transfection mimics CNE-1, CNE-2...

Studies have shown that microRNAs (miRNAs) can promote or suppress tumor growth and therefore act as targets for cancer therapy. Hsa-miR-503-5p, a mature miRNA derived from 5' ends of pre-miR-503, has been proved to regulate cell proliferation, transformation, migration invasion. However, the b iological function miR-503-3p 3' pre-miR-503 never reported. In current study, we found inhibits lung viability induces apoptosis. To better understand molecular mechanism underlying participating in...

Dysregulation of chromobox proteins contributes to the progression human diseases. CBX1 has been implicated in epigenetic control chromatin structure and gene expression, but its role cancers remains largely unknown. Here we show that exhibits oncogenic activities hepatocellular carcinoma (HCC) indicates poor outcomes. The expression was noticeably increased, at both mRNA protein levels, HCC tissues cell lines, compared with nontumorous ones. High significantly associated larger tumor size,...

// Yu-Feng Yang 1, * , Ying-Hua Pan 2, Yun Cao 3, Jia Fu 3 Xia Mei-Fang Zhang and Qiu-Hong Tian 4 1 Department of Pathology, Dongguan Third People's Hospital, Dongguan, China 2 Rheumatology Immunology, The Affiliated Hospital Sun Yat-Sen University, Guangzhou, University Cancer Center, Oncology, First Nanchang Nanchang, Jiangxi, These authors contributed equally to this work Correspondence to: Zhang, email: zhangmf@sysucc.org.cn Tian, tianqiuhong@hotmail.com Keywords: PBK, FoxM1,...

Cervical cancer (CC) is one of the most frequent gynecological malignancies in females worldwide. Aberrant expression microRNA (miR)-206 was reported to play an important role tumor progression. The aim present study evaluate potential miR-206 and verify its influence on function glucose-6-phosphate dehydrogenase (G6PD) CC. Western blot analysis RT-PCR were employed measure G6PD expression. Luciferase assays performed validate as targets. CCK-8 assay examine regulation CC proliferation....

Liver cancer ranks in prevalence and mortality among top five cancers worldwide. Accumulating interests have been focused developing new strategies for liver treatment. We previously showed that dihydroartemisinin (DHA) exhibited antitumor activity towards cancer. In this study, we demonstrated histone deacetylase inhibitors (HDACi) significantly augmented the antineoplastic effect of DHA via increasing apoptosis vitro vivo. Inhibition ERK phosphorylation contributed to DHA-induced...

PurposeThe purpose of this study is to investigate the impact transitional care by a nurse-led multidisciplinary team (MDT) on clinical outcomes and quality life patients with ankylosing spondylitis.MethodsA randomized control design was used. Subjects were allocated randomly an experimental group group. The received intensive MDT, whereas routine nursing care. Disease activity, spinal mobility, comprehensive function, health service utilization, assessed at baseline six months Bath...

Purpose To investigate the coronary venous system and its relation to adjacent structures using 256-slice computed tomography (CT). Materials Methods The study consisted of 102 patients who underwent CT angiography (CTA) CT. For each patient, were evaluated. appropriate locations diameters posterior interventricular vein (PIV), left ventricle (PVLV) marginal (LMV) detected. paired student's t test was used evaluate difference between diameter sinus (CS) ostium in anteroposterior direction...

The Optical Surface Management System (OSMS) is a simple, fast, reproducible, and accurate solution for patient set-up can minimize random day-to-day errors. However, studies in breast cancer patients are rare.To analyze 200 set-ups 20 with by comparing the OSMS conventional cone-beam computed tomography (CBCT).Displacements from concurrent CBCT registrations were compared total of setups to interfractional displacement positioning three dimensions (lateral, longitudinal, vertical...

Background: Establishment of cohesion 1 homolog 2 (ESCO2) plays important roles in the regulation and genomic stability has been implicated human cancers. Yet, its clinical significance biological function colorectal cancer (CRC) are unknown. Methods: The expression ESCO2 was examined by quantitative real-time PCR, Western blot, immunohistochemistry. role tumor metastasis CRC related mechanisms were investigated using vitro vivo models. Results: In this study, we show that low closely...

Atherosclerosis, a multifactorial chronic inflammatory response, is closely associated with oxidatively modified low-density lipoprotein (ox-LDL). High-mobility group box 1 (HMGB1) DNA-binding protein, which upon release from cells exhibits potent action. Insulin-like growth factor (IGF-1) can elicit repertoire of cellular responses including proliferation and anti-apoptosis. However, the role IGF-1 in inflammation still unclear. In present study, we aimed to investigate underlying...

Background Long non‐coding RNAs (lncRNAs) have been found to play a specific part in the development of esophageal squamous cell carcinoma (ESCC), except for lncRNA HEIH. Here, we aimed discover molecular mechanisms HEIH ESCC. Methods We detected expression level and miR‐4458 ESCC tissues cells using qRT‐PCR assay. A dual luciferase reporter assay was used check relationship between HEIH, or PBX3. Counting Clock Kit‐8 (CCK‐8) transwell were detect proliferation invasion capability. Western...

BACKGROUND The purpose of this study was to investigate the impact modified Glasgow Prognostic Score (GPS) at time recurrence on post-recurrence survival (PRS) in non-small cell lung cancer (NSCLC) patients after surgical resection. MATERIAL AND METHODS clinicopathologic characteristics and outcome data 266 with recurrent NSCLC were collected reviewed retrospectively. prognostic mGPS investigated univariate multivariate analyses. RESULTS A total analyzed. 0, 1, 2 assigned 60.9%, 33.1%, 6.0%...

Clinical diagnostic values of B-mode ultrasound and computed tomography (CT) for tuberculous pleuritis were investigated. A total 685 patients clinically diagnosed with in Yantaishan Hospital from January 2012 to August 2016 selected as study subjects. The examined by CT. accuracy CT the diagnosis was evaluated benefit-cost ratios two auxiliary methods compared. According imaging results patients, examinations identified 415 cases rate 60.15%. 501 70.07%. combined use these 546 significantly...

OBJECTIVE: Anaplastic thyroid carcinoma (ATC) is one of the most aggressive human cancers and often shows resistance to multimodal therapeutic approaches. It has been shown that transcriptional repressor Slug inhibits chemotherapeutic agent-induced apoptosis cancer cells. We evaluated wh ether targeting could augment doxorubicin (DOX)-induced ATC also determined changes in PUMA (p53-upregulated modulator apoptosis) expression levels identify possible mechanisms their combined actions....

Abstract Hepatocarcinogenesis is attributed to the reprogramming of cellular metabolism as consequence alteration in metabolite-related gene regulation. Identifying mechanism aberrant great potential provide novel targets for treatment hepatocellular carcinoma (HCC). Here, we demonstrated that glycogen synthase 2 (GYS2) restricted tumor growth HBV-related HCC via a negative feedback loop with p53. Expression GYS2 was significantly downregulated and correlated decreased content unfavorable...

Background: Physical activity helps to prevent deformities and complete training programs, is therefore benefit improve the rehabilitation prognosis of patients with ankylosing spondylitis (AS). This study aims identify potential factors affecting physical AS provide insights for clinical strategies. Methods: A cross-sectional primary data analysis was performed. Exploratory latent category (LCA) used subgroups patients. Logistic regression explore influencing activity. Results: Two (high...