A5068994994- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- SARS-CoV-2 detection and testing
- Vector-borne infectious diseases
- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Antibiotic Use and Resistance
- Antibiotic Resistance in Bacteria
- Vector-Borne Animal Diseases
- Diagnosis and treatment of tuberculosis
- Pharmaceutical and Antibiotic Environmental Impacts
- COVID-19 diagnosis using AI
- Insect and Pesticide Research
- Monoclonal and Polyclonal Antibodies Research
- Systemic Lupus Erythematosus Research
- Pneumocystis jirovecii pneumonia detection and treatment
- Advanced Biosensing Techniques and Applications
South African Medical Research Council
2020-2025
National Zoological Gardens of South Africa
2014-2018
Scientific Services
2018
University of the Free State
2014
Recent studies have shown that the detection of SARS-CoV-2 genetic material in wastewater may provide basis for a surveillance system to track environmental dissemination this virus communities. An effective wastewater-based epidemiology (WBE) prove critical South Africa (SA), where health systems infrastructure, testing capacity, personal protective equipment and human resource capacity are constrained. In proof-of-concept study, we investigated potential RNA untreated as monitor COVID-19...
The acquisition of isoniazid (INH) resistance is alarming, considering its importance as a key drug that forms the core multidrug treatment regimens for tuberculosis (TB). Genetic mutations in katG and inhA promoter regions play crucial roles INH resistance, but their prevalence varies geographically. This study aimed to identify most common genes INH-resistant (INH-R) Mycobacterium (MTB) clinical isolates Cameroon. research also explored relationships between these patients' demographics...
The performance of probiotic microorganisms is strongly affected by their environment. Bile and pH are crucial properties the as they arbitrate ability bacteria to survive in intestine. It was, therefore, important assess tolerance abovementioned factors when screening for potential. This study aimed at identifying characterizing strains isolated from wastewater treatment plants South Africa. presents very first extraction extracted using Lowenstein Jensen agar isolates were identified...
Only a few studies have examined the presence of Anaplasma marginale and centrale in South Africa, no comprehensively these species across whole country. To undertake this country-wide study we adapted duplex quantitative real-time PCR (qPCR) assay for use Africa but found that one genes on which was based variable. Therefore, sequenced variety field samples tested variants detected. We used to screen 517 cattle sourced from all nine provinces subsequently A. positive msp1α genotype gauge...
The effective control of multidrug resistant tuberculosis (MDR-TB) relies upon the timely diagnosis and correct treatment all cases. Whole genome sequencing (WGS) has great potential as a method for rapid drug Mycobacterium (Mtb) isolates. This overcomes most problems that are associated with current phenotypic susceptibility testing. However, application WGS in clinical setting been deterred by data complexities skill requirements implementing technologies well interpretation next...
Several nucleic acid-based assays have been developed for detecting Anaplasma marginale and centrale in vectors hosts, making the choice of method to use endemic areas difficult. We evaluated ability reverse line blot (RLB) hybridisation assay, two nested polymerase chain reaction (nPCR) a duplex real-time quantitative (qPCR) assay detect A. infections cattle (n = 66) South Africa. The lowest detection limits plasmid DNA were 2500 copies by RLB 250 nPCR qPCR 2500, 25 RLB, respectively....
(1) Background: This study was aimed to identify universal genetic markers of multidrug resistance (MDR) in
<title>Abstract</title> Antimicrobial resistance (AMR) has become one of the top ten global public health threat. Many countries have recognized societal and economic burden AMR. The AMR reduced effectiveness antimicrobial therapies this results to high mortality, morbidity, care expenditure. Like all other developing countries, South Africa (SA) falls under same ambiguous management system antimicrobials. A lot research been focused on threat “AMR”, however, studies in wastewater are...
Withdrawal statement This manuscript has been withdrawn by the authors as it was submitted and made public without full consent of all authors. Therefore, do not wish this work to be cited reference for project. If you have any questions, please contact corresponding author.
Summary Background The World Health Organisation recommends wastewater based epidemiology (WBE) for SARS-CoV-2 as a complementary tool monitoring population-level epidemiological features of the COVID-19 pandemic. Yet, uptake WBE in low-to-middle income countries (LMIC) is low. We report on findings from surveillance network South Africa, and make recommendations regarding implementation LMICs Methods Seven laboratories using different test methodology, quantified influent collected 87...