A5064088588- Neurogenesis and neuroplasticity mechanisms
- Pluripotent Stem Cells Research
- Axon Guidance and Neuronal Signaling
- Nerve injury and regeneration
- Animal Genetics and Reproduction
- Cerebrospinal fluid and hydrocephalus
- Reproductive Biology and Fertility
- Fetal and Pediatric Neurological Disorders
- Scientific Computing and Data Management
- MicroRNA in disease regulation
- Distributed and Parallel Computing Systems
- Neurological disorders and treatments
- Potato Plant Research
- Sperm and Testicular Function
- CRISPR and Genetic Engineering
- Mitochondrial Function and Pathology
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neuropharmacology Research
- Genetics and Neurodevelopmental Disorders
- Nuclear Receptors and Signaling
- Tropical and Extratropical Cyclones Research
- RNA Interference and Gene Delivery
- Cryptography and Data Security
- Security and Verification in Computing
- Microfluidic and Bio-sensing Technologies
University of Connecticut
2009-2024
University of Alabama in Huntsville
2002-2019
Institute of Neurobiology
2011-2017
John D. Dingell VA Medical Center
2016
Wayne State University
2016
University of Alabama
2006-2014
Jackson Laboratory
2001-2002
Medizinische Hochschule Hannover
2001
École Normale Supérieure - PSL
2000
Rockefeller University
1997-2000
The neurotrophins brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5 (NT4) act via the TrkB receptor support survival of primary somatic visceral sensory neurons. major population, nodose-petrosal ganglion complex (NPG), requires BDNF NT4 for a full complement neurons, providing unique opportunity to compare gene dosage effects between two ligands explore possibility that one ligand can compensate loss other. Analysis newborn transgenic mice lacking or NT4, revealed many NPG...
In the adult mouse brain, subventricular zone (SVZ) is a neurogenic stem cell niche only 4-5 diameters thick. Within this narrow zone, unique microenvironment supports self-renewal, gliogenesis or neurogenesis lineage decisions and tangential migration of newly generated neurons out SVZ into olfactory bulb. However, with aging, declines. Here, we examine dynamic interplay between cytoarchitecture through aging. Assembly high-resolution electron microscopy images corresponding coronal...
Through adulthood, the rodent subventricular zone (SVZ) stem cell niche generates new olfactory bulb interneurons. We had previously reported that number of neurons produced in SVZ declines through aging; however, age-related changes attributable specifically to neural (NSC) population have not been fully characterized. Here, we conducted a spatiotemporal evaluation adult NSCs. assessed ventricle-contacting NSCs, which together with ependymal cells form regenerative units (pinwheels) along...
ABSTRACT The generation of mice lacking specific neurotrophins permits evaluation the trophic requirements particular neuronal populations throughout development. In present study, we examined developing vestibulo-cochlear system to determine time course neurotrophin dependence and whether competition occurred among cochlear or vestibular neurons for available amounts either brain-derived neurotrophic factor (BDNF) neurotrophin-4/5 (NT-4/5). Both were in NT-4/5. contrast, decreased number...
The subventricular zone (SVZ) of the adult mouse brain is a narrow stem cell niche that lies along length lateral wall ventricles. SVZ supports neurogenesis throughout adulthood; however, with increasing age, ventral deteriorates and only dorsolateral remains neurogenic. Associated elderly SVZ, we reported previously an increased number astrocytes interposed within adjacent ependymal lining. Here, show integrated ependyma are dividing, BrdU-labeled share cellular adherens neighboring cells....
ABSTRACT Ciliary neurotrophic factor was discovered based on its ability to support the survival of ciliary neurons, and is now known act a variety neuronal glial populations. Two distant relatives factor, leukemia inhibitory oncostatin M, mimic with respect actions cells nervous system. In contrast M also display broad array outside The overlapping activities can be attributed shared receptor components. specificity for system results from restricted expression alpha component complex,...
Transcriptional activation of the embryonic genome occurs during two-cell stage in mouse embryo and is marked by synthesis a set α-amanitin-sensitive proteins Mr 73,000, 70,000, 68,000. We have characterized these three two-dimensional gel electrophoresis [35S]methionine radiolabeled embryos. Their isoelectric points range from 6.2 to 6.8 their synthesis, which can constitute 5–10% total protein restricted stage. These are not heat shock that previously been reported as major products...
Human brain development proceeds via a sequentially transforming stem cell population in the ventricular-subventricular zone (V-SVZ). An essential, but understudied, contributor to V-SVZ niche health is multi-ciliated ependymal epithelium, which replaces cells at ventricular surface during development. However, reorganization of and its relationship ependymogenesis has not been characterized human brain. Based on comprehensive comparative spatiotemporal analyses cytoarchitectural changes...
Summary Age‐associated ventriculomegaly is typically attributed to neurodegeneration; however, additional factors might initiate or contribute progressive ventricular expansion. By directly linking postmortem human MRI sequences with histological features of periventricular tissue, we show that substantial lateral ventricle surface gliosis associated ventriculomegaly. To examine whether loss ependymal cell coverage resulting in glial scarring can lead enlargement independent any other injury...
Mouse oocytes exposed to 1 microgram Hoechst 33342 (H-33342)/ml and then fertilized in vitro developed normally into blastocysts blastocyst outgrowths. After penetration of the zona, fertilizing spermatozoon showed intense fluorescence upon fusion with vitelline membrane. Due fluorochrome leakage from perivitelline space a faint was detected zona-bound spermatozoa. This spermatozoa intensified increased concentration (10 micrograms/ml), obscuring Spermatozoa added zona-free mouse (pre-loaded...
Coordinated regulation of the adult neurogenic subventricular zone (SVZ) is accomplished by a myriad intrinsic and extrinsic factors. The neurotransmitter dopamine one regulatory molecule implicated in SVZ function. Nigrostriatal ventral tegmental area (VTA) midbrain neurons innervate regions adjacent to SVZ, synapses are found on cells. Cell division within decreased humans with Parkinson's disease animal models following exposure toxins that selectively remove nigrostriatal neurons,...
Ventriculomegaly (expansion of the brain's fluid-filled ventricles), a condition commonly found in aging brain, results areas gliosis where ependymal cells are replaced with dense astrocytic patches. Loss would compromise trans-ependymal bulk flow mechanisms required for clearance proteins and metabolites from brain parenchyma. However, little is known about interplay between age-related ventricle expansion, decline integrity, altered periventricular fluid homeostasis, abnormal protein...
Abstract Nucleus accumbens is involved in several aspects of instrumental behavior, motivation and learning. Recent studies showed that dopamine (DA) release the shell was significantly increased on first day training a fixed ratio (FR) 5 schedule (i.e. transition from FR1 to FR5) compared with those rats continued training, even though their FR5 received less food reinforcement than continuing schedule. Additionally, second responding marked by significant increase DA core. The present...
Significant migration cues are required to guide and contain newly generated rodent subventricular zone (SVZ) neuroblasts as they transit along the lateral ventricles then through anterior forebrain their ultimate site of differentiation in olfactory bulbs (OBs). These enforce strict neuroblast spatial boundaries within dense astroglial meshwork SVZ rostral migratory stream (RMS), yet permissive large-scale migration. Therefore, molecular mechanisms that define these control dynamic...
In contrast to Whitten's medium, CZB which lacks glucose but is supplemented with glutamine, supports development of 1-cell embryos beyond the 2-cell stage for obtained from mice that exhibit block. The molecular basis this effect, however, not known. We report correlated ability medium support an enhanced rate total protein synthesis and higher level proteins reflect activation transcription embryonic genome when compared cultured in ± ethylenediaminetetraacetate (EDTA). overall patterns or...
The adult subventricular zone (SVZ) supports a population of cells that display the hallmarks stem cells: they are self-renewing and multipotent—capable generating neurons, oligodendrocytes, astrocytes. In vivo, these neural (aNSCs) fated primarily for γ-amino butyric acid (GABA)-ergic lineage olfactory bulb interneurons, small subpopulation which is dopaminergic. Here, we investigate plasticity aNSCs in vitro, particular, their ability to generate specific neuronal lineage, midbrain...