A5037693618- Glioma Diagnosis and Treatment
- Neurogenesis and neuroplasticity mechanisms
- Cancer Research and Treatments
- Ferroptosis and cancer prognosis
- Cerebrospinal fluid and hydrocephalus
- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Microtubule and mitosis dynamics
- Cancer, Hypoxia, and Metabolism
- MicroRNA in disease regulation
- Immune cells in cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Studies on Chitinases and Chitosanases
- PI3K/AKT/mTOR signaling in cancer
- Brain Metastases and Treatment
- Alzheimer's disease research and treatments
- Electrospun Nanofibers in Biomedical Applications
- Advanced Neuroimaging Techniques and Applications
- Radiopharmaceutical Chemistry and Applications
- interferon and immune responses
- Intracerebral and Subarachnoid Hemorrhage Research
- Circadian rhythm and melatonin
- Circular RNAs in diseases
Mayo Clinic in Florida
2019-2025
WinnMed
2020-2025
Johns Hopkins University
2025
Jacksonville College
2020-2024
Neurological Surgery
2021-2023
Mayo Clinic
2020
Mayo Clinic in Arizona
2020
Nemours Children's Clinic
2020
University of Connecticut
2018
University of Pittsburgh
2017
Rationale: Glioblastoma (GBM) is the most fatal form of brain cancer and its treatment represents a persistent challenge. Mesenchymal stromal cells (MSCs) have been explored as therapeutic tools in management owing to their tumor-homing abilities. However, clinical application limited due controversial role MSCs carcinogenesis. This study investigates how influence tumor behavior explores synergistic anticancer effects combination with melatonin (Mel). Methods: Orthotopic subcutaneous GBM...
Human brain development proceeds via a sequentially transforming stem cell population in the ventricular-subventricular zone (V-SVZ). An essential, but understudied, contributor to V-SVZ niche health is multi-ciliated ependymal epithelium, which replaces cells at ventricular surface during development. However, reorganization of and its relationship ependymogenesis has not been characterized human brain. Based on comprehensive comparative spatiotemporal analyses cytoarchitectural changes...
Ventriculomegaly (expansion of the brain's fluid-filled ventricles), a condition commonly found in aging brain, results areas gliosis where ependymal cells are replaced with dense astrocytic patches. Loss would compromise trans-ependymal bulk flow mechanisms required for clearance proteins and metabolites from brain parenchyma. However, little is known about interplay between age-related ventricle expansion, decline integrity, altered periventricular fluid homeostasis, abnormal protein...
Abstract Background Glioblastomas (GBMs) are the main primary brain tumors in adults with almost 100% recurrence rate. Patients lateral ventricle proximal GBMs (LV-GBMs) exhibit worse survival compared to distal locations for unknown reasons. One hypothesis is proximity of these cerebrospinal fluid (CSF) and its chemical cues that can regulate cellular phenotype. We therefore investigated role CSF on GBM gene expression a CSF-induced gene, SERPINA3, malignancy vitro vivo. Methods utilized...
Abstract Background: Several breast cancer (BC) risk prediction models are used in clinical practice to identify women eligible for enhanced screening or prevention strategies. While these have been independently validated specific and often separate contexts, their performance has not systematically evaluated compared across a wide range of populations age ranges. Methods: We collected individual-level baseline questionnaire data incident diagnoses from 14 cohorts participating the Breast...
Glioblastoma (GBM) is the most aggressive and common type of primary brain tumor in adults. Tumor location plays a role patient prognosis, with tumors proximal to lateral ventricles (LVs) presenting worse overall survival, increased expression stem cell genes, incidence distal recurrence. This may be due part interaction GBM factors subventricular zone (SVZ), including those contained within cerebrospinal fluid (CSF). However, direct CSF has not been proved would hindered presence an intact...
Glioblastoma (GBM) is the most prevalent and aggressive malignant primary brain tumor. GBM proximal to lateral ventricles (LVs) more aggressive, potentially because of subventricular zone contact. Despite this, cross-talk between neural stem/progenitor cells (NSC/NPCs) not well understood. Using cell-specific proteomics, we show that LV-proximal prevents neuronal maturation NSCs through induction senescence. In addition, tumor-initiating (BTICs) increase expression cathepsin B (CTSB) upon...
Glioblastoma (GBM) is the most common and devastating primary cancer of central nervous system in adults. High grade gliomas are able to modify respond brain microenvironment. When GBM tumors infiltrate Subventricular zone (SVZ) they have a more aggressive clinical presentation than SVZ-distal tumors. We suggest that cerebrospinal fluid (CSF) contact contributes enhance malignant characteristics these evaluated impact human CSF on GBM, performing transcriptome analysis cells exposed measure...
Despite current strategies combining surgery, radiation, and chemotherapy, glioblastoma (GBM) is the most common aggressive malignant primary brain tumor in adults. Tumor location plays a key role prognosis of patients, with GBM tumors located close proximity to lateral ventricles (LVs) resulting worse survival expectancy higher incidence distal recurrence. Though reason for these patients remains unknown, it may be due subventricular zone (SVZ) neurogenic niche contained within wall LVs. We...
Abstract Background Mesenchymal stromal cells (MSCs) have a dynamic secretome that plays critical role in tissue repair and regeneration. However, studying the MSC mixed-culture disease models remains challenging. This study aimed to develop mutant methionyl-tRNA synthetase-based toolkit (MetRS L274G ) selectively profile secreted proteins from MSCs systems demonstrate its potential for investigating responses pathological stimulation. Methods We used CRISPR/Cas9 homology-directed stably...
The vertebral column is the most common site for skeletal metastasis, often leading to debilitating pain and weakness. Metastatic cancer has unique genetic drivers that potentiate tumorigenicity. There an unmet need novel targeted therapy in patients with spinal metastatic disease. authors assessed effect of verteporfin-induced yes-associated protein (YAP) inhibition on spine cell tumorigenicity radiation sensitivity vitro. Animal studies used a subcutaneous xenograft mouse model assess use...
Background Mesenchymal stromal cells (MSCs) have a dynamic secretome that plays critical role in tissue repair and regeneration. However, studying the MSC mixed-culture disease models remains challenging. This study aimed to develop mutant methionyl-tRNA synthetase-based toolkit (MetRS L274G ) selectively profile secreted proteins from MSCs systems demonstrate its potential for investigating responses pathological stimulation. Methods We used CRISPR/Cas9 homology-directed stably integrate...
Glioblastoma (GBM) is the most prevalent and aggressive malignant primary brain tumor. GBM proximal to lateral ventricles (LVs) more aggressive, potentially due subventricular zone (SVZ) contact. Despite this, crosstalk between neural stem/progenitor cells (NSC/NPCs) not well understood. Using cell-specific proteomics, we show that LV-proximal prevents neuronal maturation of NSCs through induction senescence. Additionally, tumor initiating (BTICs) increase expression CTSB upon interaction...
Abstract The extracellular matrix (ECM) plays a critical role in cancer progression, with its biophysical properties influencing behavior. ECM stiffness can induce long-lasting adaptations cells, promoting sustained invasion through process known as “mechanical memory” (MM). Glioblastoma (GBM) exhibits spatial heterogeneity stiffness, stiffer found at the tumor periphery where invasive cells reside and promote recurrence. We hypothesize that increased primes GBM for MM. METHODS Cell lines...
Abstract Glioblastoma (GBM) is the most common malignant primary brain tumor in adults with a median survival of 8-14 months and 5-year rate 6.9%. Its aggressive nature arises mostly from molecular heterogeneity resistance to standard care (SOC), including radiation temozolomide (TMZ). Recurrence nearly inevitable, driven by highly invasive initiating cells (BTICs). Our previous studies revealed that exposing BTICs cerebrospinal fluid (CSF) GBM patients increases cell migration proliferation...
Background: Recurrent aphthous stomatitis is a common multifactorial oral mucosal disorder. Genetic and local factors causing RAS may contribute to the development of cancer. This study aims explore this connection by examining family with history ovarian Case Description: The described in case exhibits minor recurrent stomatitis. severity condition increases each generation. One member has no but was diagnosed testing for BRCA mutations negative indicating different genetic cause All...
Abstract Glioblastoma (GBM) is the most devastating and common form of primary brain cancer in adults. Tumor location plays a significant role patient prognosis; particular, GBM tumors contacting lateral ventricles (LVs) are more aggressive than LV-distal counterparts. This may be due to interaction with unique features LV microenvironment, including cerebrospinal fluid (CSF) neural progenitor cells (NPCs) subventricular zone. Despite contact having on tumor malignancy, studies using bulk...
Abstract Glioblastoma (GBM) is the most aggressive of all brain tumors with a median patient survival less than 15 months. Despite surgical resection, radiotherapy, and chemotherapy, recurrence rate almost 100%. A great percentage GBM (~60%) infiltrate contact ventricular-subventricular zone (V-SVZ). Interestingly, these are aggressive, invariably lead to higher distal rates, shorter time tumor progression, lower overall patient. The reason for this role V-SVZ-proximity on outcome patients...
Abstract Glioblastoma (GBM) is the most common and aggressive primary brain tumor in adults with a median survival of 14 months despite multimodal treatment. Patients GBM tumors exhibit post-resection recurrence rate almost 100%. The treatment resistance properties reside stem-like cell population called Brain Tumor Initiating Cells (BTIC). Among GBMs, periventricular worse than distal to lateral ventricles (LV), cause for this outcome not known. A potential explanation proximity these...