A5007383535- Circadian rhythm and melatonin
- Mitochondrial Function and Pathology
- Tryptophan and brain disorders
- Nitric Oxide and Endothelin Effects
- Coenzyme Q10 studies and effects
- Stress Responses and Cortisol
- Inflammasome and immune disorders
- Sleep and Wakefulness Research
- Genetics, Aging, and Longevity in Model Organisms
- Photoreceptor and optogenetics research
- Exercise and Physiological Responses
- Neuroendocrine regulation and behavior
- Spaceflight effects on biology
- Dietary Effects on Health
- Oral health in cancer treatment
- MicroRNA in disease regulation
- Muscle metabolism and nutrition
- Birth, Development, and Health
- Eicosanoids and Hypertension Pharmacology
- Epigenetics and DNA Methylation
- Neuroscience and Neuropharmacology Research
- Biochemical effects in animals
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Nutrition and Health in Aging
Universidad de Granada
2016-2025
Parque Tecnológico de la Salud
2016-2025
Instituto de Investigación Biosanitaria de Granada
2005-2025
Centro de Investigación Biomédica en Red de Fragilidad y Envejecimiento Saludable
2017-2025
Instituto de Salud Carlos III
2022-2025
Centro de Investigación Biomédica en Red
2017-2023
Complejo Hospitalario Universitario de Granada
2017-2019
Mayo Clinic in Florida
2019
WinnMed
2019
The University of Texas Health Science Center at San Antonio
1975-2014
We studied the subcellular levels of melatonin in cerebral cortex and liver rats under several conditions. The results show that cell membrane, cytosol, nucleus, mitochondrion vary over a 24-hr cycle, although these variations do not exhibit circadian rhythms. membrane has highest concentration followed by mitochondria, cytosol. Pinealectomy significantly increased content all compartments, whereas luzindole treatment had little effect on levels. Administration 10 mg/kg bw to...
We determined the NF-κB- and NOD-like receptor (NLR)P3-dependent molecular mechanisms involved in sepsis evaluated role of retinoid-related orphan (ROR)-α melatonin's anti-inflammatory actions. Western blot, RT-PCR, ELISA, spectrophotometric analysis revealed that NF-κB NLRP3 closely interact, leading to proinflammatory pro-oxidant status heart tissue septic C57BL/6J mice. Moreover, mitochondrial oxygen consumption was reduced by 80% In vivo vitro showed melatonin administration blunts...
Mitochondria do not contain catalase and are therefore largely dependent on reduced glutathione (GSH) peroxidases for its antioxidant protection. When GSH levels greatly decreased, hydrogen peroxide accumulates leading to extensive mitochondrial damage. Melatonin has properties prevents toxic effects of reactive oxygen species by maintaining cellular homeostasis. Thus, we examined the influence melatonin other classical antioxidants such as vitamins C E content activity GSH-related enzymes...
We tested the effect of melatonin on membrane fluidity in microsomes a rat liver model which lipid peroxidation was induced by addition FeCl 3 , ADP and NADPH. Membrane monitored using fluorescence spectroscopy estimated quantifying malonaldehyde (MDA)+4‐hydroxyalkenals (4‐HDA) concentrations following induction with without pre‐incubation (1 μ M–3 mM). rigidity increased during while reduced concentration‐dependent manner both MDA+4‐HDA generation. Melatonin's protective may relate to its...
Melatonin displays antioxidant and free radical scavenger properties. Due to its ability with which it enters cells, these protective effects are manifested in all subcellular compartments. Recent studies suggest a role for melatonin mitochondrial metabolism. To study the of on this organelle we used ruthenium red induce damage oxidative stress. The results show that (10 mg/kg i.p.) can increase activity respiratory complexes I IV after administration vivo time‐dependent manner; changes...
We evaluated the role of melatonin in endotoxemia caused by lipopolysaccharide (LPS) unanesthetized rats. The expression inducible isoform nitric oxide synthase (iNOS) and increase oxidative stress seem to be responsible for failure lungs, liver, kidneys endotoxemia. Bacterial LPS (10 mg/kg b. w) was i.v. injected 6 h before rats were killed (10-60 b.w.) i.p. and/or after LPS. Endotoxemia associated with a significant rise serum levels aspartate alanine aminotransferases,...
The role of melatonin in improving mitochondrial respiratory chain activity and increasing ATP production different experimental conditions has been widely reported. To date, however, the mechanism(s) involved are largely unknown. Using high-resolution respirometry, fluorometry spectrophotometry we studied effects on normal functions. Mitochondria were recovered from mouse liver cells incubated vitro with at concentrations ranging 1 nm to mm. Melatonin decreased oxygen consumption...
Mitochondrial nitric oxide synthase (mtNOS) is expressed constitutively, although it might be induced. Nitric (NO) a physiological regulator of mitochondrial respiration. Melatonin prevents oxidative damage and inhibits iNOS expression induced by bacterial lipopolysaccharide (LPS). The loss melatonin with age may related to the age-dependent damage. Thus, we examined protective role against effects LPS on mtNOS respiratory complexes activity in liver lung mitochondria from young old rats....
Head and neck squamous cell carcinoma (HNSCC) clearly involves activation of the Akt mammalian target rapamycin (mTOR) signalling pathway. However, effectiveness treatment with mTOR inhibitor is often limited by chemoresistance. Melatonin suppresses neoplastic growth via different mechanisms in a variety tumours. In this study, we aimed to elucidate effects melatonin on rapamycin-induced HNSCC death identify potential cross-talk pathways. We analysed dose-dependent rapamycin-treated lines...
Duchenne muscular dystrophy (DMD), a lethal disorder characterized by dystrophin absence, courses with chronic inflammation, sarcolemmal damage, and skeletal muscle degeneration. Among the multiple pathogenic mechanisms proposed for DMD, oxidative stress inflammation are directly involved in dystrophic process. Unfortunately, there is no current treatment inflammatory process an important target therapies. Based on antioxidant anti-inflammatory properties of melatonin, we investigated...
Abstract The connection between the innate immune system, clock genes, and mitochondrial bioenergetics was analyzed during aging sepsis in mouse heart. Our results suggest that sole NF ‐κB activation does not explain inflammatory process underlying aging; former also triggers NLRP 3 inflammasome enhances caspase‐1‐dependent maturation of IL ‐1β. In this way, aged mice enter into a vicious cycle as ‐1β further activates ‐κB/ link. origin related to age‐dependent Bmal1/Clock/ ROR α/Rev‐Erbα...
We tested whether chronic melatonin administration in the drinking water would reduce brain mitochondrial impairment that accompanies aging. Brain mitochondria from male and female senescent prone (SAMP8) mice at 5 10 months of age were studied. Mitochondrial oxidative stress was determined by measuring levels lipid peroxidation nitrite, glutathione/glutathione disulfide ratio, glutathione peroxidase reductase activities. Electron transport chain activity phosphorylation capability also...
Mucositis is a common and distressing side effect of chemotherapy or radiotherapy that has potentially severe consequences, no treatment available. The purpose this study was to analyze the molecular pathways involved in development oral mucositis evaluate whether melatonin can prevent pathology. tongue male Wistar rats subjected irradiation (X-ray YXLON Y.Tu 320-D03 irradiator; animals received dose 7.5 Gy/day for 5 days). Rats were treated with 45 mg/day vehicle 21 days postirradiation,...
Radiotherapy-induced gut toxicity is among the most prevalent dose-limiting toxicities following radiotherapy. Prevention of radiation enteropathy requires protection small intestine. However, despite prevalence and burden this pathology, there are currently no effective treatments for radiotherapy-induced toxicity, pathology remains unclear. The present study aimed to investigate changes induced in rat intestine after external irradiation tongue, explore potential radio-protective effects...
The NLRP3 inflammasome is involved in the innate immune response during inflammation. Moreover, melatonin blunts NF-κB/NLRP3 connection sepsis. Thus, we compared roles of and/or treatment septic wild-type and NLRP3-/- mice. Mouse myocardial tissue was used for this purpose. nuclear turnover NF-κB enhanced sepsis, with an increase TNFα, iNOS, pro-IL-1β. lack mice significantly reduced that blunted IL-1β maturation due to caspase-1. Clock Bmal1 did not change both mouse strains, enhancing...
Neural stem cells (NSCs) are regarded as a promising therapeutic approach to protecting and restoring damaged neurons in neurodegenerative diseases (NDs) such Parkinson's disease Alzheimer's (PD AD, respectively). However, new research suggests that NSC differentiation is required make this strategy effective. Several studies have demonstrated melatonin increases mature neuronal markers, which reflects into neurons. Nevertheless, the possible involvement of mitochondria effects during has...