A5058273244- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Cardiac Arrhythmias and Treatments
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Cardiomyopathy and Myosin Studies
- Atrial Fibrillation Management and Outcomes
- Cardiovascular Effects of Exercise
- Cardiac pacing and defibrillation studies
- Receptor Mechanisms and Signaling
- Acute Myeloid Leukemia Research
- ECG Monitoring and Analysis
- Advanced biosensing and bioanalysis techniques
- Viral Infections and Immunology Research
- Long-Term Effects of COVID-19
- Chronic Myeloid Leukemia Treatments
- RNA Research and Splicing
- Digital Imaging for Blood Diseases
- Cardiac Ischemia and Reperfusion
- Hematological disorders and diagnostics
- Nuclear Structure and Function
- Neuroscience and Neural Engineering
- Cystic Fibrosis Research Advances
- Cardiovascular Syncope and Autonomic Disorders
Juntendo University
2017-2024
Tokyo Medical and Dental University
2013-2024
Juntendo University Urayasu Hospital
2023-2024
Juntendo University Hospital
2017-2024
Chongqing Medical University
2024
Children's Hospital of Chongqing Medical University
2024
The Ohio State University Wexner Medical Center
2020-2022
Indiana University – Purdue University Indianapolis
2011-2021
Indiana University School of Medicine
2010-2021
The Ohio State University
2020-2021
Each of the cardiomyopathies, classically categorized as hypertrophic cardiomyopathy, dilated cardiomyopathy (DCM), and arrhythmogenic right ventricular has a signature genetic theme. Hypertrophic are largely understood diseases sarcomere or desmosome proteins, respectively. In contrast, >250 genes spanning >10 gene ontologies have been implicated in DCM, representing complex diverse architecture. To clarify this, systematic curation evidence to establish relationship with DCM was conducted.
Background— Experimental studies suggest that the interval between peak and end of T wave (Tpe) in transmural ECGs reflects dispersion repolarization (TDR), which is amplified by β-adrenergic stimulation LQT1 model. In 82 patients with genetically identified long-QT syndrome (LQTS) 33 control subjects, we examined T-wave morphology various parameters for 12-lead including corrected QT (QTc; QT/R-R 1/2 ) Tpe (Tpec; Tpe/R-R before during exercise stress tests. Methods Results— Under baseline...
Fibrillation/defibrillation episodes in failing ventricles may be followed by action potential duration (APD) shortening and recurrent spontaneous ventricular fibrillation (SVF).We hypothesized that activation of apamin-sensitive small-conductance Ca(2+)-activated K(+) (SK) channels is responsible for the postshock APD ventricles.A rabbit model tachycardia-induced heart failure was used. Simultaneous optical mapping intracellular Ca(2+) membrane (V(m)) performed nonfailing ventricles. Three...
Background We previously reported that I KAS are heterogeneously upregulated in failing rabbit ventricles and play an important role arrhythmogenesis. This study goal is to test the hypothesis subtype 2 of small‐conductance Ca 2+ activated K + ( SK 2) channel apamin‐sensitive currents ) human ventricles. Methods Results studied 12 native hearts from transplant recipients (heart failure [ HF ] group) 11 ventricular core biopsies patients with aortic stenosis normal systolic function (non‐...
The hypothesis of the Dilated Cardiomyopathy Precision Medicine Study is that most dilated cardiomyopathy has a genetic basis. study returns results to probands and, when indicated, relatives. While both American College Medical Genetics and Genomics/Association for Molecular Pathology ClinGen's MYH7-cardiomyopathy specifications provide relevant guidance variant interpretation, further gene- disease-specific considerations were required cardiomyopathy. To this end, we tailored ClinGen...
Abstract Detection of dysmorphic cells in peripheral blood (PB) smears is essential diagnostic screening hematological diseases. Myelodysplastic syndromes (MDS) are hematopoietic neoplasms characterized by dysplastic and ineffective hematopoiesis, which diagnosis mainly based on morphological findings PB bone marrow. We developed an automated support system MDS combining cell image-recognition using a deep learning (DLS) powered convolutional neural networks (CNNs) with decision-making...
Background— Subclinical mutations in genes associated with the congenital long-QT syndromes (LQTS) have been suggested as a risk factor for drug-induced LQTS and accompanying life-threatening arrhythmias. Recent studies identified genetic variants of cardiac K + channel predisposing affected individuals to acquired LQTS. We novel Na mutation an individual who exhibited Methods Results— An elderly Japanese woman documented QT prolongation torsade de pointes during treatment prokinetic drug...
Introduction: Pulmonary vein (PV) isolation has proven to be an effective therapy for atrial fibrillation (AF). However, clinical evidence suggests that suppression of AF after PV could not fully attributed the interruption electrical conduction in and out PVs. Furthermore, little is known regarding effects ablation around PVs on electrophysiological properties. We aimed study changes response vagal stimulation (VS) (PVA). Methods: studied 11 adult mongrel dogs under general anesthesia....
Background— Long-QT syndrome (LQTS) is an inherited disorder associated with sudden cardiac death. The cytoskeletal protein syntrophin-α 1 (SNTA1) known to interact the sodium channel (hNa v 1.5), and we hypothesized that SNTA1 mutations might cause phenotypic LQTS in patients genotypically normal hNa 1.5 by secondarily disturbing function. Methods Results— Mutational analysis of was performed on 39 (QTc≥480 ms) previously negative genetic screening for LQTS-causing genes. We identified a...
Abstract Acute myeloid leukemia (AML) cells are highly dependent on oxidative phosphorylation (OxPhos) for survival, and they continually adapt to fluctuations in nutrient oxygen availability the bone marrow (BM) microenvironment. We investigated how BM microenvironment affects response OxPhos inhibition AML by using a novel complex I inhibitor, IACS-010759. Cellular adhesion, growth, apoptosis assays, along with measurements of expression mitochondrial DNA generation reactive species...
Background — Mutations in the KCNJ2 gene, which codes cardiac and skeletal inward rectifying K + channels (Kir2.1), produce Andersen’s syndrome, is characterized by periodic paralysis, arrhythmia, dysmorphic features. Methods Results In 3 Japanese family members with ventricular arrhythmias, marked QT prolongation, polymerase chain reaction/single-strand conformation polymorphism/DNA sequencing identified a novel, heterozygous, missense mutation , Thr192Ala (T192A), was located putative...
Background— Apamin-sensitive K currents ( I KAS ) are upregulated in heart failure. We hypothesize that apamin can flatten action potential duration restitution (APDR) curve and reduce ventricular fibrillation failing ventricles. Methods Results— simultaneously mapped membrane intracellular Ca (Ca i 7 rabbit hearts with pacing-induced failure normal hearts. A dynamic pacing protocol was used to determine APDR at baseline after (100 nmol/L) infusion. Apamin did not change APD 80 ventricles,...
Abstract RBM20 is a major regulator of heart-specific alternative pre-mRNA splicing TTN encoding giant sarcomeric protein titin. Mutation in linked to autosomal-dominant familial dilated cardiomyopathy (DCM), yet most the missense mutations and sporadic cases were mapped an RSRSP stretch arginine/serine-rich region which function remains unknown. In present study, we identified R634W mutation within G1031X nonsense cohorts DCM patients. We demonstrate that two serine residues are...
<h3>Background:</h3> Wolff–Parkinson–White syndrome (WPW) is a bypass re-entrant tachycardia that results from an abnormal connection between the atria and ventricles. Mutations in <i>PRKAG2</i> have been described patients with familial WPW hypertrophic cardiomyopathy. Based on role of bone morphogenetic protein (BMP) signalling development annulus fibrosus mice, it has proposed BMP through type 1a receptor other downstream components may play pre-excitation. <h3>Methods results:</h3> Using...
Abstract Healthcare workers (HCWs) are highly exposed to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. The actual disease (COVID-19) situation, especially in regions that less affected, has not yet been determined. This study aimed assess the seroprevalence of SARS-CoV-2 HCWs working a frontline hospital Tokyo, Japan. In this cross-sectional observational study, screening was performed on consented HCWs, including medical, nursing, and other workers, as part...
To examine the effects of capsaicin on cystic fibrosis transmembrane conductance regulator (CFTR), we recorded wild-type and mutant CFTR chloride-channel currents using patch-clamp methods. The were compared with those genistein, a well-characterized activator. In whole-cell experiments, potentiates cAMP-stimulated expressed in NIH 3T3 cells or Chinese hamster ovary dose-dependent manner maximal response ∼60% that genistein an apparent <i>K</i><sub>d</sub> 48.4 ± 6.8 μM. cell-attached...
Dilated cardiomyopathy (DCM) is a primary disease of the heart muscle associated with sudden cardiac death secondary to ventricular tachyarrhythmias and asystole. However, molecular pathways linking DCM arrhythmias are unknown. We previously identified S196L mutation in exon 4 LBD3-encoded ZASP family death. These findings led us hypothesize that this may precipitate both cytoskeletal conduction abnormalities vivo. Therefore, we investigated role ZASP4 cytoarchitecture ion channel biology.We...
The CFTR chloride channel is activated by phosphorylation of serine residues in the regulatory (R) domain and then gated ATP binding hydrolysis at nucleotide domains (NBDs). Studies ATP-dependent gating process excised inside-out patches are very often hampered rundown partly caused membrane-associated phosphatases. Since severed ΔR-CFTR, whose R completely removed, can bypass phosphorylation-dependent regulation, this mutant might be a useful tool to explore mechanisms CFTR. To end, we...
Defects of cytoarchitectural proteins can cause left ventricular noncompaction, which is often associated with conduction system diseases. We have previously identified a p.D117N mutation in the LIM domain-binding protein 3-encoding Z-band alternatively spliced PDZ motif gene (ZASP) patient noncompaction and disturbances. sought to investigate role LBD3 NM_001080114.1 isoform (ZASP1-D117N) for regulation cardiac sodium channel (Na(v)1.5) that plays an important system.Effects ZASP1-wild-type...
We examined the usefulness of five COVID-19 antibody detection tests using 114 serum samples at various time points from 34 Japanese patients. Elecsys Anti-SARS-CoV-2 Roche, and four immunochromatography Hangzhou Laihe Biotech, Artron Laboratories, Chil, Nadal. In first week after onset, had 40% positivity in Group S (severe cases) but was negative M (mild-moderate cases). The kits showed 40–60% 0–8% Groups M, respectively. second week, 75% 50% positivity, 5–80% 50–75% After third 100% both...