A5053042487- Mitochondrial Function and Pathology
- Metabolism, Diabetes, and Cancer
- Helicobacter pylori-related gastroenterology studies
- Endoplasmic Reticulum Stress and Disease
- Metabolism and Genetic Disorders
- Pancreatic function and diabetes
- Stress Responses and Cortisol
- Adipose Tissue and Metabolism
- Estrogen and related hormone effects
- Signaling Pathways in Disease
- Birth, Development, and Health
- RNA regulation and disease
- Neonatal and fetal brain pathology
- Infant Nutrition and Health
- Clinical Nutrition and Gastroenterology
- Lipid metabolism and biosynthesis
- Galectins and Cancer Biology
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Immunodeficiency and Autoimmune Disorders
- Reproductive System and Pregnancy
- Toxoplasma gondii Research Studies
- RNA modifications and cancer
- Histone Deacetylase Inhibitors Research
- Herpesvirus Infections and Treatments
- Hormonal Regulation and Hypertension
Johns Hopkins Medicine
2022-2024
Johns Hopkins University
2022-2024
University of Nebraska Medical Center
2024
Bharathidasan University
2014-2018
Vanderbilt University Medical Center
2018
ABSTRACT Bacterial lipopolysaccharide (LPS) is the most important contributing factor in pathogenesis of bacterial infection male accessory glands; and it has shown to inhibit testicular steroidogenesis induce apoptosis. The present study demonstrates that LPS causes mitochondrial dysfunction via suppression sirtuin 4 (SIRT4); which turn affects Leydig cell function by modulating LC‐540 cells treated with (10µg/ml) showed impaired increased cellular mRNA protein expression SIRT4 were...
Inhibition of P300 acetyltransferase activity by specific inhibitor C646 has been shown to improve insulin signaling. However, the underlying molecular mechanism this improvement remains unclear. In study, we analyzed levels obese patients and found that they were significantly increased in liver hepatocytes. addition, large amounts appeared cytoplasm. drastically tyrosine phosphorylation receptor protein substrates (IRS1/2) without affecting beta subunit (IRβ) hepatocytes absence insulin....
Anemia is a common and serious health problem, nearly universally diagnosed in preterm infants, associated with increased morbidity mortality worldwide. Red blood cell (RBC) transfusion lifesaving mainstay therapy; however, it has critical adverse effects. One consequence necrotizing enterocolitis (NEC), an inflammatory bowel necrosis disease infants. The murine model of phlebotomy-induced anemia RBC transfusion-associated NEC enables detailed study the molecular mechanisms underlying these...
Mitochondria are highly dynamic organelles of bacterial origin in eukaryotic cells. These play a central role metabolism and adenosine triphosphate (ATP) synthesis the production regulation reactive oxygen species (ROS). In addition to generation energy, mitochondria perform numerous other functions support key developmental events such as fertilization during reproduction, oocyte maturation, development embryo. During embryonic neonatal development, may have important effects on metabolic,...
Mitochondrial dynamic process is important for cell viability, metabolic activity, and mitochondria health. Here, we present a protocol measuring mitochondrial size through immunofluorescence staining, confocal imaging, analysis in ImageJ. We describe the steps tissue processing, antigen retrieval, staining using an integrating assay, computerized image to measure each mouse human liver tissues. This reduces sample volume processing time preparation of primary cells. For complete details on...
Far-western blotting, derived from the western blot, has been used to detect interactions between proteins in vitro, such as receptor-ligand interactions. The insulin signaling pathway plays a critical role regulation of both metabolism and cell growth. binding receptor substrate (IRS) is essential for propagation downstream after activation by insulin. Here, we describe step-by-step far-western blotting protocol determining IRS receptor.