A5112309624- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Hepatitis Viruses Studies and Epidemiology
- Bacteriophages and microbial interactions
- Veterinary medicine and infectious diseases
- Parasitic Infections and Diagnostics
- Gut microbiota and health
- Animal Disease Management and Epidemiology
- Viral Infections and Vectors
- Toxoplasma gondii Research Studies
- Liver Disease and Transplantation
- Ruminant Nutrition and Digestive Physiology
- Escherichia coli research studies
- Circular RNAs in diseases
- Respiratory viral infections research
- RNA modifications and cancer
- Genomics and Phylogenetic Studies
- Virus-based gene therapy research
- Streptococcal Infections and Treatments
- Bacterial Identification and Susceptibility Testing
- Hepatitis B Virus Studies
- Herpesvirus Infections and Treatments
- Clostridium difficile and Clostridium perfringens research
- Microbial infections and disease research
- Rabies epidemiology and control
Tibet University
2011-2024
Shanghai Jiao Tong University
2009-2011
South China Agricultural University
2011
Abstract Background Wild birds may harbor and transmit viruses that are potentially pathogenic to humans, domestic animals, other wildlife. Results Using the viral metagenomic approach, we investigated virome of cloacal swab specimens collected from 3182 (the majority them wild species) consisting > 87 different species in 10 orders within Aves classes. The virus diversity was higher than breeding birds. We acquired 707 genomes 18 defined families 4 unclassified groups, with 265 sharing...
Abstract Background Yaks are able to utilize the gastrointestinal microbiota digest plant materials. Although cellulolytic bacteria in yak rumen have been reported, there is still limited information on diversity of major microorganisms and putative carbohydrate-metabolizing enzymes for degradation complex lignocellulosic biomass its gut ecosystem. Results Here, this study aimed decode biomass-degrading genes genomes fecal using deep metagenome sequencing. A comprehensive catalog comprising...
Tibetan pig is an important domestic mammal, providing products of high nutritional value for millions people living in the Qinghai-Tibet Plateau. The genomes mammalian gut microbiota encode a large number carbohydrate-active enzymes, which are essential digestion complex polysaccharides through fermentation. However, current understanding microbial degradation dietary carbohydrates limited. In this study, we produced approximately 145 gigabases metagenomic sequence data fecal samples from...
Several animal species can reportedly act as reservoirs for Hepatitis E virus (HEV), a zoonotic pathogen. HEV and antibody to the have been detected in variety of animals including rodents. Pig rat models established HEV, but nude mouse has not yet developed. Balb/c mice were inoculated with swine both orally via intravenous injection insure infection. Negative control experimental contact-exposed groups also included study. The liver, spleen, kidney, jejunum, ileum, cecum colon each from...
ABSTRACT Genomic and evolutionary analysis of epidemic porcine hepatitis E virus (HEV) in the Tibetan Plateau was performed. Faecal samples were collected from 216 pigs 78 Yorkshire (Large White) 53 tissue Linzhi City slaughterhouse. Total RNA extracted faeces fragments HEV open reading frame 2 (ORF2) detected by reverse transcription nested polymerase chain reaction (RT‐nPCR) cloned. Twenty‐three (23/347; 6.63%) positive for virus, including 6.94% (15/216) pig 6.11% (8/131) samples. No...
The gut virome is a reservoir of diverse symbiotic and pathogenic viruses coevolving with their hosts, yet limited research has explored the viromes highland-dwelling rare species. Using viral metagenomic analysis, communities Phrynocephalus lizards living in Qinghai-Tibet Plateau were investigated. Phage-encoded functional genes antibiotic resistance (ARGs) analyzed. different lizard species all predominated by bacteriophages, especially Caudovirales order. erythrurus around Namtso Lake...
Calf diarrhea is one of the common diseases involved in process calf feeding. In this study, a sample that tested positive for bovine coronavirus and astrovirus was subjected to high-throughput sequencing. The reassembly revealed complete genomes norovirus, astrovirus, kobuvirus, S gene coronavirus. Phylogenetic analysis showed ORF2 region had lowest similarity with other strains gathered Mamastrovirus unclassified genogroup, suggesting new serotype/genotype could appear. Compared most...
Toxoplasma gondii infection is widely prevalent in humans and animals, including pigs throughout the world. In this study, seroprevalence of T. Tibetan China was investigated for first time. A total 427 serum samples were collected from Nyingchi prefecture, Tibet, between April December 2010, assayed antibodies to using modified agglutination test (MAT). Ninety-seven (22.72%) found be positive with MAT titers 1:25 or higher. Slaughter had highest seroprevalence, compared fattening pigs,...
In the present investigation, seroprevalence of Toxoplasma gondii antibodies in 455 Tibetan sheep Tibet, China, was examined using an indirect hemagglutination test. Of these, 26 (5.7%) were seropositive at cut-off 1:64 serum dilution. The ranged from 2.2% to 8.9% among <1-yr-old, 1–3-yr-old, and >3-yr-old, but differences age groups not significant (P > 0.05). prevalence male (2.8%) lower than that female (6.6%), difference statistically results this survey indicated presence T. infection...
Clostridium perfringens (C. perfringens) is an opportunistic pathogen that cause necrotic enteritis, food poisoning and even death in animals. In this study, we explored the prevalence, antibiotic resistance genetic diversity of isolated from yak Qinghai-Tibet plateau, China. A total 744 fecal samples were collected assessed for toxin genes, antimicrobial susceptibility multilocus sequence typing (MLST). Results indicated 144 out (19.35%) tested to be positive C. , 75% ( n = 108, 108/144)...
ABSTRACT HEV infection has become a global health concern. The study of pathogenicity been hindered by the lack suitable in vitro culture system. In present research, we systematic demonstration efficient replication swine GT4 A549 cells, Huh‐7 and HepG2/C3A cell lines. results immunofluorescence assay confocal showed that is efficiently replicated three lines at 72 h postinoculation. Meanwhile, also detected virus titer quantified were increased 2‐, 6,‐ 11‐days Moreover, successfully...
The present study examined the seroprevalence of Mycoplasma hyopneumoniaeinfection in Tibetan pigs China for first time. Serum samples were collected randomly from 423 of different categories Nyingchi county and Mainling County, Tibet, April to December 2010, then tested presence antibodies against M. hyopneumoniae by double-sandwich enzyme-linked immunosorbent assay (ELISA). results showed that 249 (58.86%) positive ranged 25.00 75.00% age type groups. There was a higher...
Hepatitis E virus, Tibet, Swine, Positive rate
This article has been retracted by the publisher.
旨在探究牦牛Nramp1基因mRNA及可能靶定Nramp1的miRNAs组织表达谱。本研究利用RT-PCR技术对牦牛Nramp1基因的mRNA组织表达谱进行分析，同时运用TargentScan和miRBase软件预测牦牛可能靶定Nramp1基因的miRNAs，并利用加PloyA尾法RT-PCR技术分析miRNAs在肝、脾、肺、肾、后腿骨骼肌、卵巢、小肠、颌下淋巴结、大肠、肠系淋巴结10种组织中的相对表达量。试验结果显示，Nramp1基因mRNA在所检测的10种组织中均有表达，其中在脾、颌下淋巴结及肺组织中的表达量极显著高于肝、肾、大肠、肠系淋巴结组织（P 0.05），而bta-miR-106a、bta-miR-20b在颌下淋巴结、肠系淋巴结组织中的表达量极显著高于脾组织表达量（P < 0.01），bta-miR-106b在颌下淋巴结组织中的表达量极显著高于肠系淋巴结组织表达量（P...
为了明确西藏部分地区绵羊体外寄生羊蜱蝇携带病原巴尔通体的感染情况，作者于2019年1-9月采集了西藏林芝、日喀则、那曲地区绵羊体外寄生羊蜱蝇298只，通过形态学鉴定、PCR扩增羊蜱蝇18S rRNA基因进行虫体鉴定，并对病原巴尔通体的gltA基因进行检测，将部分阳性PCR产物连接pMDTM-18T后转入DH5α感受态细胞，将阳性结果进行测序并进行遗传进化分析。结果显示，雌性阳性率49.8%（113/227），雄性阳性率42.3%（30/71），雌雄之间差异不显著（χ2=0.944，P=0.267）；羊蜱蝇携带病原巴尔通体的总感染率为48.0%（143/298），林芝极显著高于日喀则、那曲地区（χ2=13.801，P < 0.01；χ2=17.067，P 0.01），日喀则和那曲地区无显著差异（χ2=0.084，P=0.771）；散养模式羊蜱蝇携带阳性率44.3%（102/230），圈养阳性率85.0%（34/40），屠宰场阳性率23.3%（7/30），宿主散养和圈养、屠宰场存在极显著差异（χ2=20.929，P 0.01；χ2=24.38，P...