A5001425937- Lipid Membrane Structure and Behavior
- Surfactants and Colloidal Systems
- RNA Interference and Gene Delivery
- Electrostatics and Colloid Interactions
- Dendrimers and Hyperbranched Polymers
- Force Microscopy Techniques and Applications
- Polymer Surface Interaction Studies
- Advanced biosensing and bioanalysis techniques
- Antimicrobial Peptides and Activities
- Supramolecular Self-Assembly in Materials
- Protein Structure and Dynamics
- Spectroscopy and Quantum Chemical Studies
- Lipoproteins and Cardiovascular Health
- Nanopore and Nanochannel Transport Studies
- Proteins in Food Systems
- Protein purification and stability
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Metabolomics and Mass Spectrometry Studies
- Cardiac Imaging and Diagnostics
- Cancer, Lipids, and Metabolism
- Nanocomposite Films for Food Packaging
- Microfluidic and Capillary Electrophoresis Applications
- Advanced Drug Delivery Systems
- Molecular Junctions and Nanostructures
- DNA and Nucleic Acid Chemistry
Ikerbasque
2023-2025
Instituto Biofisika
2022-2025
Malmö University
2015-2024
University of the Basque Country
2023-2024
Consejo Superior de Investigaciones Científicas
2023-2024
Fundación Biofísica Bizkaia
2022-2024
Nanyang Technological University
2022-2024
BioSurfaces (United States)
2015-2020
University of Copenhagen
2011-2017
KTH Royal Institute of Technology
2008
Emerging therapeutic treatments based on the production of proteins by delivering mRNA have become increasingly important in recent times. While lipid nanoparticles (LNPs) are approved vehicles for small interfering RNA delivery, there still challenges to use this formulation delivery. LNPs typically a mixture cationic lipid, distearoylphosphatidylcholine (DSPC), cholesterol, and PEG-lipid. The structural characterization mRNA-containing (mRNA-LNPs) is crucial full understanding way which...
Polylactide (PLA) is viewed as a potential material to replace synthetic plastics (e.g., poly(ethylene terephthalate) (PET)) in food packaging, and there have been number of developments this direction. However, for PLA be competitive more demanding uses such the packaging oxygen-sensitive foods, oxygen permeability coefficient (OP) needs reduced by factor ∼10. To achieve this, layer-by-layer (Lbl) approach was used assemble alternating layers montmorillonite clay chitosan on extruded film...
The demand for highly efficient macromolecular drugs, used in the treatment of many severe diseases, is continuously increasing. However, hydrophilic character and large molecular size these drugs significantly limit their ability to permeate across cellular membranes thus impede reaching target sites body. Cell-penetrating peptides (CPP) have gained attention as promising drug excipients, since they can facilitate permeation cell constituting a major biological barrier. Fluorophores are...
Lipid nanoparticles (LNPs) are the most versatile and successful gene delivery systems, notably highlighted by their use in vaccines against COVID-19. LNPs have a well-defined core–shell structure, each region with its own distinctive compositions, suited for wide range of vivo applications. Here, we discuss how detailed knowledge LNP structure can guide formulation to improve efficiency nucleic acid payload. Perspectives on structural design more efficient transfection. Views key physical...
We have investigated the effect of deposition temperature on supported lipid bilayer formation via vesicle fusion. By using several complementary surface-sensitive techniques, we demonstrate that despite contradicting literature subject, high-quality bilayers can be formed below main phase-transition lipid. carefully studied mechanism DPPC and above melting (Tm) by quartz crystal microbalance atomic force microscopy under continuous flow conditions. also measured structure or Tm neutron...
In this paper we present a systematic study of the morphology and composition supported lipid bilayers (SLBs) formed by vesicle fusion using wide variety surface sensitive techniques that give information about lateral as well vertical structure bilayer fluidity. SLBs 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) mixtures at five different bulk compositions were in such way phase separation boundaries crossed. For all studied,...
Peptide-based liquid droplets (coacervates) produced by spontaneous liquid-liquid phase separation (LLPS), have emerged as a promising class of drug delivery systems due to their high entrapping efficiency and the simplicity formulation. However, detailed mechanisms governing interaction with cell membranes cellular uptake remain poorly understood. In this study, we investigated interactions peptide coacervates composed HB pep —peptide derived from histidine-rich beak proteins (HBPs)...
This paper describes a combined investigation of the salivary and MUC5B films structure topography in conditions similar to those found oral cavity terms ionic strength, pH, protein concentration. AFM ellipsometry were successfully used give detailed picture film both on hydrophilic hydrophobic substrata. Regardless substrata, can be described as having two sublayer which an inner dense layer is decorated by large aggregates. However, shape height these larger aggregates largely depend type...
Nanodiscs are self-assembled ∼50-nm2 patches of lipid bilayers stabilized by amphipathic belt proteins. We demonstrate that a well ordered dense film nanodiscs serves for non-destructive, label-free studies isolated membrane proteins in native like environment using neutron reflectometry (NR). This method exceeds vesicle or supported bilayer because can be selectively adsorbed with controlled orientation. As proof concept, the mechanism action membrane-anchored cytochrome P450 reductase...
Dendrimers are polymers with a strong role in nanomedicine. In the current work we have developed platform for mapping out biomolecule corona polyamidoamine (PAMAM) dendrimers. Complement proteins including C3 and C4b were found high generation dendrimers, suggesting affinities to dendrimers most importantly complement activation.
Supported lipid bilayers are widely used for sensing and deciphering biomolecular interactions with model cell membranes. In this paper, we present a method to form supported from total extracts of Escherichia coli by vesicle fusion. We show the validity different types including those deuterated biomass using combination complementary surface sensitive techniques; quartz crystal microbalance, neutron reflection atomic force microscopy. find that head group composition hydrogenated is...
Arrays of nanoparticle-supported lipid bilayers (nanoSLB) are lipid-coated nanopatterned interfaces that provide a platform to study curved model biological membranes using surface-sensitive techniques. We combined scattering techniques with direct imaging, gain access sub-nanometer scale structural information on stable nanoparticle monolayers assembled silicon crystals in noncovalent manner Langmuir-Schaefer deposition. The structure supported formed the arrays via vesicle fusion was...
This paper presents a comprehensive evaluation using Z-test to assess the 1 effectiveness of an adaptive Least Mean Squares (LMS) filter driven by Steepest Descent 2 Method (SDM). The study utilizes male voice recording, captured in controlled studio 3 environment, which persistent Gaussian noise was intentionally introduced, simulating 4 real-world interference. All signal processing methods were implemented accordingly 5 MATLAB. demonstrated significant improvement 20 dB 6 Signal-to-Noise...
Starch-modifying processes, such as pyrodextrinization, are potential ways to alter the nutritional features of this polysaccharide. A widely used method for pyrodextrinizing maize starch was also applied lentil, sorghum, cocoyam, sagu, and cassava starches, in vitro digestibility products evaluated. Pyrodextrins were produced by heating at 140 °C 3 h, with catalytic amounts HCl. The enzymatically available content all preparations decreased 55−65% after modification. Thus,...
The contribution of nonspecific interactions to the overall thiol-ssDNA and dsDNA macromolecules with gold nanoparticles was investigated. A systematic investigation utilizing dynamic light scattering cryogenic transmission electron microscopy has been performed directly measure visualize changes in particle size appearance during functionalization nonthiolated dsDNA. results show that both do stabilize nanoparticle dispersions, but possible between hydrophobic DNA bases surface promote...
3-Aminopropylsilane (APS) coupling agent is widely used in industrial, biomaterial, and medical applications to improve adhesion of polymers inorganic materials. However, during exposure elevated humidity temperature, the deposited APS layers can decompose, leading reduction efficiency, thus decreasing product quality mechanical strength polymer–inorganic material interface. Therefore, a better understanding chemical state stability on surfaces needed. In this work, we investigated silica...