A5017409967- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Apelin-related biomedical research
- Viral Infections and Immunology Research
- Cytokine Signaling Pathways and Interactions
- Cardiovascular Disease and Adiposity
- RNA Research and Splicing
- Biochemical effects in animals
- Pain Mechanisms and Treatments
- RNA Interference and Gene Delivery
- Neuropeptides and Animal Physiology
- Hormonal Regulation and Hypertension
- Immunodeficiency and Autoimmune Disorders
- Genetic Neurodegenerative Diseases
- Thermal Expansion and Ionic Conductivity
- Pharmacological Effects of Natural Compounds
- Peripheral Neuropathies and Disorders
- Cancer Treatment and Pharmacology
- Chronic Kidney Disease and Diabetes
- interferon and immune responses
- S100 Proteins and Annexins
- Mitochondrial Function and Pathology
- Axon Guidance and Neuronal Signaling
- RNA regulation and disease
- Neurogenesis and neuroplasticity mechanisms
- RNA and protein synthesis mechanisms
Universidad de Oviedo
2023
University of Lisbon
2021-2023
Universidade Nova de Lisboa
2021-2023
Czech Academy of Sciences
2015-2020
Czech Academy of Sciences, Institute of Physiology
2019-2020
Université de Rouen Normandie
2008-2010
Inserm
2008-2010
Institute for Research and Innovation in Biomedicine
2008
Laboratoire de Neurobiologie Cellulaire et Moléculaire
2007
Différenciation et Communication Neuronale et Neuroendocrine
2006
Abstract Paclitaxel‐induced peripheral neuropathy (PIPN) is often associated with neuropathic pain and neuroinflammation in the central nervous system. Antihypertensive drug losartan, an angiotensin II receptor type 1 (AT1R) blocker, was shown to have anti‐inflammatory neuroprotective effects disease models, predominantly via activation of peroxisome proliferator‐activated gamma (PPARγ). Here, effect systemic losartan treatment (100 mg/kg/d) on mechanical allodynia evaluated rat PIPN model....
UII (urotensin II) and its paralogue URP (UII-related peptide) are two vasoactive neuropeptides whose respective central actions currently unknown. In the present study, we have compared mechanism of action on cultured astrocytes. Competition experiments performed with [125I]UII showed presence very-high- high-affinity binding sites for UII, a single site URP. Both provoked membrane depolarization accompanied by decrease in input resistance, stimulated release endozepines, specifically...
Abstract The urotensin II (UII) gene is primarily expressed in the central nervous system, but functions of UII brain remain elusive. Here, we show that cultured rat astrocytes constitutively express receptor (UT). Saturation and competition experiments performed with iodinated ([ 125 I]rUII) revealed presence high‐ low‐affinity binding sites on astrocytes. Human (hUII) two highly active agonists hUII 4‐11 [3‐iodo‐Tyr9]hUII were also very potent displacing [ I]rUII from its sites, whereas...
Abstract After peripheral nerve injury microglial reactivity change in the spinal cord is associated with an early activation of Janus kinase ( JAK )/ STAT 3 transduction pathway whose blockade attenuates local inflammation and pain hypersensitivity. However, consequences / 3‐mediated signaling on neighboring cells are unknown. Using vitro paradigm we assessed impact activity functional characteristics astrocytes neurons. Purified rat primary microglia was stimulated classical activator...
Autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS) is a neurodegenerative disorder commonly diagnosed in infants and characterized by progressive cerebellar ataxia, spasticity, motor sensory neuropathy axonal demyelination. ARSACS caused mutations the SACS gene that lead to truncated or defective forms 520 kDa multidomain protein, sacsin. Sacsin function exclusively studied on neuronal cells, where it regulates mitochondrial network organization facilitates normal...
Abstract Astroglial cells synthesize and release endozepines, a family of neuropeptides derived from diazepam‐binding inhibitor (DBI). The authors have recently shown that β‐amyloid peptide (Aβ) stimulates DBI gene expression endozepine release. purpose this study was to determine the mechanism action Aβ in cultured rat astrocytes. 25–35 N ‐formyl receptor (FPR) agonist ‐formyl‐Met‐Leu‐Phe (fMLF) increased secretion endozepines dose‐dependent manner with EC 50 value ≈2 μM. stimulatory...
STAT3 is a pleiotropic transcription factor overactivated in 70% of solid tumours. We have recently reported that inactivating mutations on residues susceptible to post-translational modifications (PTMs) only one the monomers (i.e. asymmetric) caused changes cellular distribution homodimers. Here, we used more controlled experimental conditions, i.e. without interference endogenous (STAT3-/- HeLa cells) and presence defined cytokine stimulus (Leukemia Inhibitory Factor, LIF), provide further...
Abstract STAT3 is a pleiotropic transcription factor overactivated in 70% of solid tumours. We have recently reported that inactivating mutations on residues susceptible to post-translational modifications (PTMs) only one the monomers (i.e. asymmetric) caused changes cellular distribution homodimers. Here, we used more controlled experimental conditions, i.e. without interference endogenous (STAT3-/- HeLa cells) and presence defined cytokine stimulus (Leukemia Inhibitory Factor, LIF),...