A5000063112- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Hormonal Regulation and Hypertension
- Apelin-related biomedical research
- Adrenal and Paraganglionic Tumors
- Neuropeptides and Animal Physiology
- Cancer, Hypoxia, and Metabolism
- Cardiovascular Disease and Adiposity
- Cancer-related molecular mechanisms research
- Neuroblastoma Research and Treatments
- MicroRNA in disease regulation
- Thermal Expansion and Ionic Conductivity
- Hypothalamic control of reproductive hormones
- Neuroendocrine regulation and behavior
- Cellular transport and secretion
- Receptor Mechanisms and Signaling
- Carcinogens and Genotoxicity Assessment
- Pituitary Gland Disorders and Treatments
- RNA modifications and cancer
- Circular RNAs in diseases
- Stress Responses and Cortisol
- Brain Metastases and Treatment
- Ovarian function and disorders
- Cancer Cells and Metastasis
- Melanoma and MAPK Pathways
- Metabolism, Diabetes, and Cancer
Université de Rouen Normandie
2008-2023
Inserm
2011-2023
Université Sultan Moulay Slimane
2023
Différenciation et Communication Neuronale et Neuroendocrine
2007-2022
Normandie Université
2007-2022
Germinal (United Kingdom)
2022
Neuroendocrine, Endocrine and Germinal Differentiation Communication
2022
Public Risk Management Association
2021
Institute for Research and Innovation in Biomedicine
2014-2020
Université de Caen Normandie
2007
microRNAs are noncoding RNAs which downregulate a large number of target mRNAs and modulate cell activity. Despite continued progress, bioinformatics prediction microRNA targets remains challenge since available software still suffer from lack accuracy sensitivity. Moreover, these tools show fairly inconsistent results one another. Thus, in an attempt to circumvent difficulties, we aggregated all human four important algorithms (miRanda, PITA, SVmicrO, TargetScan) showing additional...
Urotensin II (UII) is an evolutionarily conserved neuropeptide initially isolated from teleost fish on the basis of its smooth muscle-contracting activity. Subsequent studies have demonstrated occurrence several UII-related peptides (URPs), such that UII family now known to include four paralogue genes called UII, URP, URP1 and URP2. These probably arose through two rounds whole genome duplication occurred during early vertebrate evolution. URP has been identified both in tetrapods teleosts....
The sequence of human urotensin II (UII) has been recently established as H-Glu-Thr-Pro-Asp-Cys-Phe-Trp-Lys-Tyr-Cys-Val-OH, and it reported that UII is the most potent mammalian vasoconstrictor peptide identified so far. A series analogues was synthesized, contractile activity each compound studied in vitro using de-endothelialised rat aortic rings. Replacement amino acid by an l-alanine or a d-isomer showed N- C-terminal residues flanking cyclic region amidated were relatively tolerant to...
A number of incidentally discovered pheochromocytomas are not associated with hypertension. The characteristics normotensive (NIPs) poorly known.The purpose this work was to assess the clinical, hormonal, histological, and molecular features NIPs.This a retrospective cohort recruited from 2001 2011 in 2 tertiary care medical departments.Clinical, biological, radiological investigations performed 96 consecutive patients sporadic unilateral were examined; 47 had overt responsible for Among...
The vasoactive peptide urotensin II (UII) is primarily expressed in motoneurons of the brainstem and spinal cord. Intracerebroventricular injection UII provokes various behavioral, cardiovascular, motor, endocrine responses rat, but distribution receptor central nervous system (CNS) has not yet been determined. In present study, we have investigated localization (GPR14) mRNA binding sites rat CNS. RT-PCR analysis revealed that highest density GPR14 occurred pontine nuclei. situ hybridization...
The urotensin II (UII) family is currently known to consist of two paralogous peptides, namely UII and UII-related peptide (URP). In contrast UII, which has been identified in all vertebrate classes so far, URP only characterized tetrapods. We report here the occurrence distinct genes teleosts, we have named URP1 URP2. Synteny analysis revealed that teleost URP2 tetrapod represent three paralog that, together with gene, probably arose from rounds tetraploidization, took place early...
Abstract The urotensin II (UII) gene is primarily expressed in the central nervous system, but functions of UII brain remain elusive. Here, we show that cultured rat astrocytes constitutively express receptor (UT). Saturation and competition experiments performed with iodinated ([ 125 I]rUII) revealed presence high‐ low‐affinity binding sites on astrocytes. Human (hUII) two highly active agonists hUII 4‐11 [3‐iodo‐Tyr9]hUII were also very potent displacing [ I]rUII from its sites, whereas...
Urotensin II (UII) and UII-related peptide (URP) are paralog neuropeptides whose existence distribution in mouse have not yet been investigated. In this study, we showed by HPLC/RIA analysis that the UII-immunoreactive molecule brain corresponds to a new UII(17) isoform. Moreover, calcium mobilization assays indicated URP were equally potent stimulating UII receptor (UT receptor). Quantitative RT-PCR situ hybridization revealed CNS mRNAs predominantly expressed brainstem spinal motoneurons....
26RFa is a new member of the RFamide peptide family that has been identified as endogenous ligand orphan GPCR GPR103. As C-terminal heptapeptide (26RFa((20-26))) mimics action native on food intake and gonadotropin secretion in rodents, we have synthesized series analogues 26RFa((20-26)) measured their potency to induce [Ca(2+)](i) mobilization Gα(16)-hGPR103-transfected CHO cells. Systematic replacement each residue by an alanine (Ala scan) its D-enantiomer (D showed last three residues...
Abstract Aldosterone, produced by the adrenals and under control of plasma angiotensin potassium levels, regulates hydromineral homeostasis blood pressure. Here we report that neuropeptide substance P (SP) released intraadrenal nerve fibres, stimulates aldosterone secretion via binding to neurokinin type 1 receptors (NK1R) expressed aldosterone-producing adrenocortical cells. The action SP is mediated extracellular signal-regulated kinase pathway involves upregulation steroidogenic enzymes....
Urotensin II (U-II) is a potent vasoconstrictor peptide which has been identified as the endogenous ligand for orphan G protein-coupled receptor GPR14 now renamed UT receptor. As C-terminal cyclic hexapeptide of U-II (U-II(4-11), H-Asp-Cys-Phe-Trp-Lys-Tyr-Cys-Val-OH) possesses full biological activity, we have synthesized series U-II(4-11) analogues and measured their binding affinity on hGPR14-transfected CHO cells contractile activity de-endothelialized rat aortic rings. The data indicate...
The European eel (Anguilla anguilla) presents a blockade of sexual maturation at pre-pubertal stage due to deficient production gonadotropins. We previously initiated, in the eel, investigation kisspeptin system, one major gatekeepers puberty mammals, and we predicted sequence two Kiss genes. In present study, cloned sequenced Kiss1 Kiss2 cDNAs from brain. tissue distributions transcripts, as investigated by qPCR, showed that both genes are primarily expressed brain pituitary. 10-residue...
Oxytocin is a neuropeptide produced mainly in the hypothalamus and secreted CNS blood. In brain, it plays major role promoting social interactions. Here we show that human plasma about 60% of oxytocin naturally bound to IgG which modulates receptor signaling. Further, found violent aggressive inmates were characterized by lower affinity for oxytocin, causing decreased carrier capacity reduced activation as compared men from general population. Moreover, peripheral administration together...
Starting from nonpeptide agonists and antagonists of human urotensin-II (hU-II), several pharmacophores were designed compared to the structure hU-II. NMR dynamic studies realized on hU-II urotensin-II-related peptide check conformation flexibilities these peptides relationships between their potential 3D structures pharmacophores. In parallel, a virtual screening was carried out, leading discovery six new derivatives with micromolar affinities. This last result shows interest for ligands.
Background: The mechanisms by which pregnancy may unmask pheochromocytomas and paragangliomas are not totally understood. We hypothesized that gestational hormones participate in the pathophysiology of catecholamine excess during pregnancy. report a case silent pheochromocytoma revealed pregnant woman life-threatening adrenergic myocarditis. Methods: In vitro studies were conducted to investigate effect estradiol hormone hCG (human chorionic gonadotropin) on epinephrine secretion cultured...
26RFa, the endogenous QRFPR ligand, is implicated in several physiological and pathological conditions such as regulation of glucose homeostasis bone mineralization; hence, ligands display therapeutic potential. At molecular level, functional interaction occurs between residues Arg25 26RFa Gln125 QRFPR. We have designed 26RFa(20–26) analogues incorporating arginine derivatives modified by alkylated substituents. found that side chain length was necessary to retain activity N-monoalkylation...