A5032653668- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Neurobiology and Insect Physiology Research
- Chemical Synthesis and Analysis
- Regulation of Appetite and Obesity
- Hypothalamic control of reproductive hormones
- RNA Interference and Gene Delivery
- Neuroscience and Neuropharmacology Research
- Nanoparticle-Based Drug Delivery
- Molecular Biology Techniques and Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Pancreatic function and diabetes
- Cephalopods and Marine Biology
- Insect and Pesticide Research
- Insect and Arachnid Ecology and Behavior
- Antimicrobial Peptides and Activities
- Selenium in Biological Systems
- Plant Reproductive Biology
- Amino Acid Enzymes and Metabolism
- Fluorine in Organic Chemistry
- Peptidase Inhibition and Analysis
- Neurological Disease Mechanisms and Treatments
- Marine Bivalve and Aquaculture Studies
- Biochemical Analysis and Sensing Techniques
- Adipose Tissue and Metabolism
Université de Rouen Normandie
2015-2025
Inserm
2016-2025
Normandie Université
2010-2025
Institute for Research and Innovation in Biomedicine
2012-2024
Différenciation et Communication Neuronale et Neuroendocrine
2013-2023
Neuroendocrine, Endocrine and Germinal Differentiation Communication
2023
Public Risk Management Association
2014
Institute for Economic Research
2010-2012
Abstract Aim Selenoprotein T (SelT or SELENOT ) is a novel thioredoxin‐like enzyme whose genetic ablation in mice results early embryonic lethality. SelT exerts an essential cytoprotective action during development and after injury through its redox‐active catalytic site. This study aimed to determine the expression regulation of mammalian heart normal pathological conditions evaluate cardioprotective effect SelT‐derived peptide, SelT43‐52( PSELT encompassing redox motif which key function,...
Hypothalamic glucose sensing is involved in the control of feeding behavior and peripheral homeostasis, glial cells are suggested to play an important role this process. Diazepam-binding inhibitor (DBI) its processing product octadecaneuropeptide (ODN), collectively named endozepines, secreted by astroglia, ODN a potent anorexigenic factor. Therefore, we investigated involvement endozepines brain sensing. First, showed that intracerebroventricular administration rats increases DBI expression...
The radical SAM enzyme AlbA has been reported to catalyze the formation of a thioether bond in antibiotic subtilosin A. By modeling, biochemical and synthetic approaches, we propose novel mechanistic perspectives on this emerging group enzymes.
Ribosomally synthesized and post-translationally modified peptides (RiPPs) are a growing family of bioactive peptides. Among RiPPs, the bacterial toxin polytheonamide A is characterized by unique set post-translational modifications catalyzed novel radical S-adenosyl-l-methionine (SAM) enzymes. Here we show that SAM enzyme PoyD catalyzes in vitro epimerization C-to-N directional manner. By combining mutagenesis experiments with labeling studies investigating substrate promiscuity, deciphered...
Cardiac lipotoxicity is an important contributor to cardiovascular complications during obesity. Given the fundamental role of endoplasmic reticulum (ER)-resident Selenoprotein T (SELENOT) for cardiomyocyte differentiation and protection regulation glucose metabolism, we took advantage a small peptide (PSELT), derived from SELENOT redox-active motif, uncover mechanisms through which PSELT could protect cardiomyocytes against lipotoxicity. To this aim, modeled cardiac by exposing H9c2...
Using an integrated transcriptomic and proteomic approach, we characterized the venom peptidome of European red ant, Manica rubida. We identified 13 "myrmicitoxins" that share sequence similarities with previously ant peptides, one them being as EGF-like toxin likely resulting from a threonine residue modified by O-fucosylation. Furthermore, conducted insecticidal assays reversed-phase HPLC fractions on blowfly Lucilia caesar, permitting us to identify six myrmicitoxins (i.e., U3-, U10-,...
Abstract A series of four fluorinated dipeptide analogues each containing a fluoro‐olefin moiety as peptide bond surrogate has been designed and synthesized. These motifs have successfully introduced into the bioactive C‐terminal heptapeptide neuropeptide 26RFa by conventional SPPS. We then evaluated ability generated pseudopeptides to increase [Ca 2+ ] i in GPR103‐transfected cells. For these analogues, greater stability human serum was observed. Their conformations were also investigated,...
Gut bacteria play key roles in intestinal physiology, via the secretion of diversified bacterial effectors. Many these effectors remodel host proteome, either by altering transcription or regulating protein post-translational modifications. SUMOylation, a ubiquitin-like modification playing is target gut bacteria. Mutualistic can promote production short- branched-chain fatty acids (SCFA/BCFA). In contrast, several pathogenic were shown to dampen SUMOylation order infection. Here, we...
Restrictive anorexia nervosa (AN) presents an adaptive appetite regulating profile including mainly high levels of ghrelin. Because this mechanism is not effective on food intake, other appetite-regulating peptides need to be explored. 26RFa a hypothalamic neuropeptide that stimulates appetite, gonadotropin release, and bone metabolism. The objective the study was evaluate circadian in AN patients compared with healthy subjects, eating disorders, constitutional thinness (CT). This...
26RFa is a new member of the RFamide peptide family that has been identified as endogenous ligand orphan GPCR GPR103. As C-terminal heptapeptide (26RFa((20-26))) mimics action native on food intake and gonadotropin secretion in rodents, we have synthesized series analogues 26RFa((20-26)) measured their potency to induce [Ca(2+)](i) mobilization Gα(16)-hGPR103-transfected CHO cells. Systematic replacement each residue by an alanine (Ala scan) its D-enantiomer (D showed last three residues...
Parkinson's disease (PD) is a neurodegenerative disorder characterized by motor dysfunction for which there an unmet need better treatment options. Although oxidative stress common feature of diseases, notably PD, currently no efficient therapeutic strategy able to tackle this multi-target pathophysiological process. Based on our previous observations the potent antioxidant and neuroprotective activity SELENOT, vital thioredoxin-like selenoprotein, we designed small peptide PSELT from its...
BackgroundP17, a peptide isolated from Tetramorium bicarinatum ant venom, is known to induce an alternative phenotype of human monocyte–derived macrophages via activation unknown G protein–coupled receptor (GPCR).ObjectiveWe sought investigate the mechanism action and immunomodulatory effects P17 mediated through MRGPRX2 (Mas-related X2).MethodsTo identify GPCR for P17, we screened 314 GPCRs. Upon identification MRGPRX2, battery in silico, vitro, ex vivo, vivo assays along with mutation...
Intranasal (IN) administration appears to be a suitable route for clinical use as it allows direct delivery of bioactive molecules the central nervous system, reducing systemic exposure and sides effects. Nevertheless, only some can transported brain from nasal cavity. This led us compare efficiency an IN, intravenous (IV), intraperitoneal (IP) pituitary adenylate cyclase-activating polypeptide (PACAP) after transient or permanent middle cerebral artery occlusion (MCAO) in C57BL/6 mice. The...
Abstract Maladaptive cardiac hypertrophy contributes to the development of heart failure (HF). The oxidoreductase Selenoprotein T (SELENOT) emerged as a key regulator during rat cardiogenesis and acute protection. However, its action in chronic settings dysfunction is not understood. Here, we investigated role SELENOT pathophysiology HF: (i) by designing small peptide (PSELT), recapitulating activity via redox site, assessed beneficial preclinical model HF [aged spontaneously hypertensive...
26RFa, a novel RFamide neuropeptide, is the endogenous ligand of former orphan receptor GPR103. Intracerebroventricular injection 26RFa and its C-terminal heptapeptide, 26RFa((20-26)), stimulates food intake in rodents. To develop potent, stable ligands GPR103 with low molecular weight, we have designed series aza-β(3)-containing 26RFa((20-26)) analogues for their propensity to establish intramolecular hydrogen bonds, evaluated ability increase [Ca(2+)](i) GPR103-transfected cells. We...
In mammals, neurokinin B (NKB) is a short peptide encoded by the gene tac3. It involved in brain control of reproduction stimulating GnRH neurons, mainly via kisspeptin. We investigated tac3 genes and peptides basal teleost, European eel, which shows an atypical blockade sexual maturation at pre-pubertal stage. Two paralogous (tac3a tac3b) were identified eel genome, each encoding two (NKBa or b NKB-related NKB-RPa b). Amino-acid sequence NKBa identical to human NKB, three others are novel...
Extensins are hydroxyproline-rich glycoproteins thought to strengthen the plant cell wall, one of first barriers against pathogens, through intra- and intermolecular cross-links. The glycan moiety extensins is believed confer correct structural conformation glycoprotein, leading self-assembly within wall that helps limit microbial adherence invasion. However, this role not clearly established.We used Arabidopsis thaliana mutants impaired in extensin arabinosylation investigate root-microbe...
Despite its major importance in human health, the metabolic potential of gut microbiota is still poorly understood. We have recently shown that biosynthesis Ruminococcin C (RumC), a novel ribosomally synthesized and posttranslationally modified peptide (RiPP) produced by commensal bacterium
Biofilms are highly tolerant to antimicrobials and host immune defense, enabling pathogens thrive in hostile environments. The diversity of microbial biofilm infections requires alternative complex treatment strategies. In a previous work we demonstrated that the human Atrial Natriuretic Peptide (hANP) displays strong anti-biofilm activity toward Pseudomonas aeruginosa binding hANP by AmiC protein supports this effect. This sensor has been identified as an analog natriuretic peptide receptor...
The peptide toxins in the venoms of small invertebrates such as stinging ants have rarely been studied due to limited amount venom available per individual. We used a venomics strategy identify molecular diversity peptidome for myrmicine ant Tetramorium bicarinatum. methodology included (i) peptidomics, which peptides are sequenced through de novo mass spectrometry approach or Edman degradation; (ii) transcriptomics, based on RT-PCR-cloning and DNA sequencing; (iii) data mining RNA-seq...
The European eel (Anguilla anguilla) presents a blockade of sexual maturation at pre-pubertal stage due to deficient production gonadotropins. We previously initiated, in the eel, investigation kisspeptin system, one major gatekeepers puberty mammals, and we predicted sequence two Kiss genes. In present study, cloned sequenced Kiss1 Kiss2 cDNAs from brain. tissue distributions transcripts, as investigated by qPCR, showed that both genes are primarily expressed brain pituitary. 10-residue...
exDNA is found in various organisms, including plants. However, plant has thus far received little attention related to its origin and role the RET (root extracellular trap). In this study, we performed first high-throughput genomic sequencing of from a Fabaceae with worldwide interest: soybean (Glycine max (L.) Merr.). The was investigated control condition, results show high-coverage on organelles (mitochondria/plastid) DNA relative nuclear DNA, as well mix coding non-coding sequences....
Huntington's disease (HD) is a hereditary neurodegenerative disorder caused by the expression of mutant huntingtin (mHtt). One main features HD degeneration striatum that leads to motor discoordination. Pituitary adenylate cyclase-activating polypeptide (PACAP) neuropeptide acts through three receptors named PAC1R, VPAC1R, and VPAC2R. In present study, we first investigated effect PACAP on STHdhQ7/Q7 STHdhQ111/Q111 cells express wild-type Htt with 7 mHtt 111 glutamines, respectively. Then...