A5085315764- Glaucoma and retinal disorders
- Ocular Surface and Contact Lens
- Chemokine receptors and signaling
- Lymphatic System and Diseases
- Biochemical effects in animals
- Corneal Surgery and Treatments
- Adipose Tissue and Metabolism
- Advanced Fluorescence Microscopy Techniques
- Cardiovascular Health and Disease Prevention
- Retinoids in leukemia and cellular processes
- Neuroinflammation and Neurodegeneration Mechanisms
- Regulation of Appetite and Obesity
- Mercury impact and mitigation studies
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Hormonal Regulation and Hypertension
- Retinal Diseases and Treatments
- Apelin-related biomedical research
- Advanced Glycation End Products research
- Alzheimer's disease research and treatments
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- interferon and immune responses
- Birth, Development, and Health
- Receptor Mechanisms and Signaling
- Mesenchymal stem cell research
Inserm
2016-2025
Différenciation et Communication Neuronale et Neuroendocrine
2012-2024
Institute for Research and Innovation in Biomedicine
2019-2024
Neuroendocrine, Endocrine and Germinal Differentiation Communication
2024
Université de Rouen Normandie
2019-2023
Université Sultan Moulay Slimane
2023
Normandie Université
2017-2020
Centre National de la Recherche Scientifique
2011-2018
Institut de la Vision
2009-2018
Sorbonne Université
2008-2018
Clearing techniques have been developed to transparentize mouse brains, thereby preserving 3D structure, but their complexity has limited use. Here, we show that immunolabeling of axonal tracts followed by optical clearing with solvents (3DISCO) and light-sheet microscopy reveals brain connectivity in embryos postnatal brains. We the Robo3 receptor is selectively expressed medial habenula axons forming fasciculus retroflexus (FR) analyzed development this commissural tract mutants Slit/Robo...
Lymphatics play an essential pathophysiological role in promoting fluid and immune cell tissue clearance. Conversely, cells may influence lymphatic function remodeling. Recently, cardiac lymphangiogenesis has been proposed as a therapeutic target to prevent heart failure after myocardial infarction (MI). We investigated the effects of gene therapy modulate post-MI rodents. Second, we determined impact cardiac-infiltrating T on remodeling heart. Approach Results: Comparing adenoviral versus...
Abstract Introduction Glaucoma is a sight-threatening retinal neuropathy associated with elevated intraocular pressure (IOP) due to degeneration and fibrosis of the trabecular meshwork (TM). medications aim reduce IOP without targeting specific TM pathology, Bone-marrow mesenchymal stem cells (MSCs) are used today in various clinical studies. Here, we investigated potential MSCs therapy an glaucoma-like ocular hypertension (OHT) model decipher vitro effects on primary human cells. Methods...
Alzheimer’s disease (AD) is characterized by extracellular deposits of amyloid β (Aβ) peptide and intracellular tau aggregates. Examination these lesions in post-mortem brain tissue routinely provides a two-dimensional picture the pathology with limited depth field. Several tissue-clearing protocols have been recently published [1, 4–6, 8, 10, 11, 16]. We compared quality immunostaining on human tissues using several clearing techniques including CLARITY [4, 5], Scale [8], SeeDB [9], 3DISCO...
Glaucoma is one of the leading causes irreversible blindness in world. The major risk factor elevated intraocular pressure (IOP) to progressive retinal ganglion cell (RGC) death from optic nerve (ON) visual pathways brain. has been reported share mechanisms with neurodegenerative disorders. We therefore hypothesize that neuroinflammatory central may contribute spread glaucoma disease. aim present study was analyze neuroinflammation processes occur pathological retina superior colliculi (SCs)...
Lymphatics are essential for cardiac health, and insufficient lymphatic expansion (lymphangiogenesis) contributes to development of heart failure (HF) after myocardial infarction. However, the regulation impact lymphangiogenesis in non-ischaemic cardiomyopathy following pressure-overload remains be determined. Here, we investigated transversal aortic constriction (TAC) C57Bl/6 Balb/c mice, end-stage HF patients.Cardiac function was evaluated by echocardiography, hypertrophy, lymphatics,...
The renal lymphatic vasculature and the endothelial cells that make up this network play important immunomodulatory roles during inflammation. How lymphatics respond to AKI may affect outcomes. authors used single-cell RNA sequencing characterize mouse in quiescent cisplatin-injured kidneys. Lymphatic cell gene expression changes were confirmed ischemia-reperfusion injury cultured cells, validating data. This study is first describe heterogeneity uncovers molecular pathways demonstrating...
Usher syndrome type 1 (USH1) is a major cause of inherited deafness and blindness in humans. The eye disorder often referred to as retinitis pigmentosa, which characterized by secondary cone degeneration following the rod loss. development treatments prevent retinal has been hampered lack clear evidence for mutant mice deficient Ush1 genes, instead faithfully mimic hearing deficit. We show that, under normal housing conditions, Ush1g-/- Ush1c-/- albino have dysfunctional photoreceptors...
Tear hyperosmolarity is known to cause ocular surface inflammation in dry eye syndrome. Benzalkonium chloride (BAK), an eyedrop preservative, induce long-term-treated patients. Analyzing the modulation of proinflammatory potential presence BAK on conjunctiva could give new insights into effect this preservative disease. In a hyperosmolar model conjunctiva-derived cell line, and BAK, we evaluated key inflammatory markers [CCL2, IL-8, IL-6, macrophage migration inhibitory factor (MIF)...
Cardiac lymphatics have emerged as potential targets in cardiovascular diseases (CVDs). However, we recently reported that despite extensive lymphatic expansion during experimental cardiac pressure-overload, drainage remained insufficient. To unravel the cellular and molecular mechanisms underlying dysfunction CVDs, applied single-cell (sc) analyses a murine heart failure model. Transaortic constriction (TAC), C57BL/6J BALB/c mice, was used to model chronic pressure-overload-induced...
The present study aims to investigate the impact of orexin deficiency on regulation energy and glucose metabolism using a mouse model depleted for prepro-orexin gene. Our data reveal that, despite decrease food consumption (at least in males), induces significant increase body weight that is associated with an alteration composition, as males females deficient mice display increased fat mass compared wild-type littermates. Nevertheless, no differences global expenditure locomotor activity...
Industrial pollution due to heavy metals such as mercury is a major concern for the environment and public health. Mercury, in particular methylmercury (MeHg), primarily affects brain development neuronal activity, resulting neurotoxic effects. Because chemokines can modulate functions are involved neuroinflammatory neurodegenerative diseases, we tested possibility that effect of MeHg may interfere with chemokine CCL2. We have used an original protocol young mice using MeHg-contaminated...
Parkinson's disease (PD) is a neurodegenerative disorder characterized by motor dysfunction for which there an unmet need better treatment options. Although oxidative stress common feature of diseases, notably PD, currently no efficient therapeutic strategy able to tackle this multi-target pathophysiological process. Based on our previous observations the potent antioxidant and neuroprotective activity SELENOT, vital thioredoxin-like selenoprotein, we designed small peptide PSELT from its...
Glaucoma, the most common cause of irreversible blindness, is a neuropathy commonly initiated by pathological ocular hypertension due to unknown mechanisms trabecular meshwork degeneration. Current antiglaucoma therapy does not target causal pathology, which may explain why treatment failure often observed. Here we show that chemokine CXCL12, its truncated form SDF-1(5-67), and receptors CXCR4 CXCR3 are expressed in human glaucomatous tissue cell line. SDF-1(5-67) produced under control...
Introduction 26RFa (pyroglutamyl RFamide peptide (QRFP)) is a biologically active that has been found to control feeding behavior by stimulating food intake, and regulate glucose homeostasis acting as an incretin. The aim of the present study was thus investigate impact gene knockout on regulation energy metabolism. Research design methods mutant mice were generated homologous recombination, in which entire coding region prepro26RFa replaced iCre sequence. Energy metabolism evaluated through...