A5013062344- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Hypothalamic control of reproductive hormones
- Regulation of Appetite and Obesity
- Neuroendocrine regulation and behavior
- Diabetes Treatment and Management
- Cancer Genomics and Diagnostics
- Sleep and Wakefulness Research
- Biochemical Analysis and Sensing Techniques
- CRISPR and Genetic Engineering
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Stress Responses and Cortisol
- Circadian rhythm and melatonin
- Lipid Membrane Structure and Behavior
- Lung Cancer Treatments and Mutations
- Neuroendocrine Tumor Research Advances
- Neurobiology and Insect Physiology Research
- Axon Guidance and Neuronal Signaling
- Pancreatic function and diabetes
- Hormonal Regulation and Hypertension
- Cellular transport and secretion
- Eating Disorders and Behaviors
- Cancer, Stress, Anesthesia, and Immune Response
- Prostate Cancer Diagnosis and Treatment
- Historical and Archaeological Studies
Inserm
2006-2023
Université de Rouen Normandie
2003-2023
Institute for Research and Innovation in Biomedicine
2014-2023
American Dental Education Association
2023
John Wiley & Sons (United States)
2023
Différenciation et Communication Neuronale et Neuroendocrine
1999-2021
Normandie Université
2016-2018
Hôpitaux Universitaires Henri-Mondor
2018
Institut de Recherche et d’Innovation
2014-2016
Chonnam National University
2006
Abstract The novel RFamide peptide 26RFa, the endogenous ligand of orphan receptor GPR103, affects food intake, locomotion, and activity gonadotropic axis. However, little is known regarding localization 26RFa receptors. present report provides first detailed mapping binding sites GPR103 mRNA in rat central nervous system (CNS). were widely distributed brain spinal cord, whereas expression was more discrete, notably midbrain, pons, medulla oblongata, suggesting that can bind to a receptor(s)...
26RFa is a hypothalamic RFamide neuropeptide that was identified as the endogenous ligand of orphan G protein-coupled receptor, GPR103, and stimulates appetite in mice. Up until now, mechanism action control food intake remains unknown. The high density GPR103 arcuate nucleus (Arc) prompted us to investigate, present study, effects on rat Y (NPY)/proopiomelanocortin (POMC) system. Intracerebroventricular injection stimulated NPY expression release basal hypothalamus, whereas it decreased...
Pituitary adenylate cyclase-activating polypeptide (PACAP) exerts trophic effects on several neuronal, neuroendocrine, and endocrine cells. To gain insight into the pattern of transcriptional modifications induced by PACAP during cell differentiation, we studied this neuropeptide rat pheochromocytoma PC12 We first analyzed transcriptome cells in comparison to that terminally differentiated adrenomedullary chromaffin cells, using a high-density microarray, identify genes associated with...
Abstract To determine the possible role of pituitary adenylate cyclase‐activating polypeptide (PACAP) in development sympathoadrenal cell lineage, we have examined effects this neurotrophic peptide, comparison to nerve growth factor (NGF), on morphology, electrophysiological properties, expression neuronal and neuroendocrine marker genes, activity transcription factors during differentiation sympathoadrenal‐derived cells, using rat pheochromocytoma PC12 model. Both PACAP NGF elicited rapid...
Restrictive anorexia nervosa (AN) presents an adaptive appetite regulating profile including mainly high levels of ghrelin. Because this mechanism is not effective on food intake, other appetite-regulating peptides need to be explored. 26RFa a hypothalamic neuropeptide that stimulates appetite, gonadotropin release, and bone metabolism. The objective the study was evaluate circadian in AN patients compared with healthy subjects, eating disorders, constitutional thinness (CT). This...
The structure of the neuropeptide pituitary adenylate cyclase-activating polypeptide (PACAP) has been characterized in several species including protochordates, fish, amphibians, birds, and mammals. Although PACAP shown to stimulate frog adrenal cell activity, precursor expression its gene have not yet reported any amphibian species. In this study, we two cDNA variants encoding Rana ridibunda, one which encodes a second peptide exhibiting strong homologies growth hormone-releasing hormone...
Secretogranin II (SgII) is an acidic secretory protein present in large dense core vesicles of neuronal and endocrine cells. Based on the sequence a peptide derived from processing SgII brain frog Rana ridibunda , degenerate oligonucleotides were used to clone cDNA encoding pituitary library. This encodes 574 amino acid which exhibits 46–48% identity with mammalian contains 11 pairs basic acids. Four potential products delimited by residues exhibited much higher degree (68–82%) corresponding...
Immune-autonomic interactions are known to occur at the level of adrenal medulla, and be important in immune stress responses, but molecular signaling pathways through which cytokines actually affect chromaffin cell function unknown. Here, we studied effects proinflammatory cytokines, TNF-alpha IL-1, on gene transcription secretion bioactive neuropeptides, primary bovine adrenochromaffin cells. IL-1 induced a time- dose-dependent increase galanin, vasoactive intestinal polypeptide,...
The neurohypophysial nonapeptides vasopressin (VP) and oxytocin (OT) modulate a broad range of cognitive social activities. Notably, in amphibians, vasotocin (VT), the ortholog mammalian VP, plays crucial role control sexual behaviors. Because several neurosteroids also regulate reproduction-related behaviors, we investigated possible effect VT OT mesotocin (MT) neurosteroid production. Double immunohistochemical labeling frog brain sections revealed presence VT/MT-positive fibers close...
26RFa is a hypothalamic neuropeptide that promotes food intake. upregulated in obese animal models, and its orexigenic activity accentuated rodents fed high-fat diet, suggesting this might play role the development maintenance of status. As obesity frequently associated with type 2 diabetes, we investigated whether may be involved regulation glucose homeostasis. In current study, show moderate positive correlation between plasma levels insulin patients diabetes. Plasma concentration also...
Secretoneurin (SN) is a novel bioactive peptide that derives from the neuroendocrine protein secretogranin II (SgII) by proteolytic processing and participates in neuro-immune communication. The neuropeptide pituitary adenylate cyclase-activating polypeptide (PACAP-38) dose-dependently stimulates (EC<sub>50</sub> ∼ 3 nM) SN release (up to 4-fold) SgII gene expression 60-fold) cultured bovine adrenochromaffin cells. effect of PACAP on both secretion mRNA levels rapid long lasting. We analyzed...
Pituitary adenylate cyclase-activating polypeptide (PACAP) exerts its various effects through activation of two types G protein-coupled receptors, a receptor with high affinity for PACAP named PAC1-R and receptors exhibiting similar both vasoactive intestinal VPAC1-R VPAC2-R. Here, we report the characterization novel splice variants in frog Rana ridibunda. The has 78% homology human is highly expressed central nervous system. Two that display additional amino acid cassettes third...
Three receptor subtypes for the neuropeptides vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating polypeptide (PACAP) have been identified in mammals: PAC1 (PAC1-R) which is selectively activated by PACAP, two VPAC receptors (VPAC1-R VPAC2-R), are equally stimulated PACAP VIP. The structures of VIP well conserved during evolution, but little known about VIP/PACAP nonmammalian species. An amphibian complementary DNA (cDNA) has cloned characterized from a frog (Rana...
Although the short-term effects of fasting or energy deficit on hypothalamic neuropeptide circuitries are now better understood, long-term and refeeding remain to be elucidated. We showed that after a deficit,<b> </b>mice exhibited persistent hypoleptinemia following period despite restoration fat mass, ovarian activity, feeding behavior. aimed examine adaptations 10 weeks further nutritional recovery. To do so, we assessed mRNA levels leptin receptor main orexigenic anorexigenic...
Three receptor subtypes for the neuropeptides vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating polypeptide (PACAP) have been identified in mammals: PAC1 (PAC1-R) which is selectively activated by PACAP, two VPAC receptors (VPAC1-R VPAC2-R), are equally stimulated PACAP VIP. The structures of VIP well conserved during evolution, but little known about VIP/PACAP nonmammalian species. An amphibian complementary DNA (cDNA) has cloned characterized from a frog (Rana...
Pituitary adenylate cyclase-activating polypeptide (PACAP) exerts its various effects through activation of two types G protein-coupled receptors, a receptor with high affinity for PACAP named PAC1-R and receptors exhibiting similar both vasoactive intestinal VPAC1-R VPAC2-R. Here, we report the characterization novel splice variants in frog Rana ridibunda. The has 78% homology human is highly expressed central nervous system. Two that display additional amino acid cassettes third...