Surface-initiated atom transfer radical polymerization (ATRP) has been used to prepare antifouling copolymer brushes based on 2-(2-methoxyethoxy)ethyl methacrylate (MEO2MA) and hydroxyl-terminated oligo(ethylene glycol) (HOEGMA). The amount of hydroxyl reactive groups incorporated into the was varied by changing composition monomer mixture. These coatings were subsequently functionalized a natural antibacterial peptide, magainin I, via an oriented chemical grafting groups, which maintains...

The composition of temperature-responsive copolymer brushes based on oligo(ethylene glycol) methacrylates is tuned to obtain a collapse temperature ∼35 °C. chains are grafted by antibacterial magainin I peptide, whose activity tested at different temperatures against various bacteria. switch from bactericidal cell-repellent below and slightly above 35 °C, respectively, due the progressive vertical brush. Detailed facts importance specialist readers published as "Supporting Information". Such...

SAG12 is the most widely used senescence-associated reference gene for characterizing leaf senescence, and increase in protein during senescence remarkable. However, role of this cysteine protease N remobilization process remains unclear. The has been poorly investigated few reports dealing with are somewhat controversial. Indeed, sag12 Arabidopsis mutants have not shown any phenotype, while OsSAG12-1 OsSAG12-2 overexpression rice moderates progression. Therefore, study aims at clarifying...

Abstract A non‐water‐soluble natural antibacterial peptide, gramicidin A, has been successfully incorporated into polyelectrolyte assemblies to elaborate biocidal thin films. For this, we used a double strategy, the first step of which consists complexing peptide by non‐denaturing anionic amphiphilic polysaccharide, namely hydrophobically modified carboxymethylpullulan. We demonstrate that use this derivative allows efficiently solubilize in aqueous solution, without denaturation. The amount...

The members of the genus Acinetobacter are Gram-negative cocobacilli that frequently found in environment but also hospital setting where they have been associated with outbreaks nosocomial infections. Among them, baumannii has emerged as most common pathogenic species involved hospital-acquired One reason for this emergence may be its persistence wards, particular intensive care unit; could partially explained by capacity these microorganisms to form biofilm. Therefore, our main objective...

The clinical importance of Acinetobacter baumannii is partly due to its natural ability survive in the hospital environment. This persistence may be explained by capacity form biofilms and, interestingly, A. can pellicles at air-liquid interface more readily than other less pathogenic species. Pellicles from twenty-six strains were morphologically classified into three groups: I) egg-shaped (27%); II) ball-shaped (50%); and III) irregular (23%). One strain representative each group was...

The increasing threat of Acinetobacter baumannii as a nosocomial pathogen is mainly due to the occurrence multidrug-resistant strains that are associated with real problem its eradication from hospital wards. particular ability this form biofilms contributes persistence, increases antibiotic resistance, and promotes persistent/device-related infections. We previously demonstrated virstatin, which small organic compound known decrease virulence Vibrio cholera via an inhibition T4-pili...

Pseudomonas aeruginosa is a multi-drug-resistant human opportunistic pathogen largely involved in nosocomial infections. Unfortunately, effective antibacterial agents are lacking. Exploring its physiology at the post-translational modifications (PTMs) level may contribute to renewal of combat tactics. Recently, lysine succinylation was discovered bacteria and seems be an interesting PTM. We present first succinylome acetylome P. PA14 cultured presence four different carbon sources using 2D...

Acinetobacter baumannii has emerged as an opportunistic nosocomial pathogen causing infections worldwide. One reason for this emergence is due to its natural ability survive in the hospital environment, which may be explained by capacity form biofilms. Cell surface appendages are important determinants of A. biofilm formation and such constitute interesting targets prevent development biofilm-related infections. A chemical agent called virstatin was recently described impair virulence Vibrio...

It has been recently shown that resistance to both imipenem and meropenem in multidrug-resistant clinical strains of Acinetobacter baumannii is associated with the loss a heat-modifiable 25/29-kDa outer membrane protein, called CarO. This study aimed investigate channel-forming properties Mass spectrometry analyses this protein band detected another 25-kDa (called Omp25), together Both proteins presented similar physicochemical parameters (M(w) pI). We overproduced purified two polypeptides...

Abstract Our goal was to identify the leaf proteomic changes which appeared during N remobilisation that were associated or not with senescence of oilseed rape in response contrasting nitrate availability. Remobilisation and status followed using 15 tracing, patterns chlorophyll level, total protein content a molecular indicator based on expression senescence‐associated gene 12/ Cab genes. Three phases distinguished. Proteomics revealed 55 proteins involved metabolism, energy,...

Bacillus subtilis B38, isolated from soil, showed antimicrobial activity against human pathogenic Candida albicans species. Specific PCR primers revealed the presence of bamC gene, which is involved in biosynthesis bacillomycin D. Three anti-Candida compounds designated a(1) , a(2) and a(3) were purified culture supernatant identified using matrix-assisted laser desorption/ionization time-of-flight MS as analogues D-like lipopeptides 14, 15 16 carbon fatty acid long chains, respectively. The...

Background Interestingly, Acinetobacter baumannii presents an enhanced capacity to form biofilms (also named pellicles) at the air-liquid interface as compared other species. This characteristic questions contribution of this phenotype increased risk clinical infections by pathogen. Methodology/Principal Findings By a proteomic approach using 2-D gel electrophoresis-LC-MS/MS mass spectrometry, we membrane protein patterns A. 77, pellicle-forming isolate, grown in planktonic and sessile...

ObjectivesIn the context of increasing worldwide occurrence imipenem-resistant Acinetobacter baumannii strains, we investigated a possible porin-mediated mechanism relating to carbapenem resistance-associated outer membrane protein, CarO. The aim this study was determine whether porin may be diffusion pathway for carbapenems in A. baumannii.

Bacteria cells within biofilms are physiologically distinct from their planktonic counterparts. In particular they more resistant to detrimental environmental conditions. this study, we monitored the evolution of phospholipid composition inner and outer membranes P. aeruginosa during biofilm formation (i.e., 1-, 2-, 6-day-old biofilm). Lipidome analyses were performed by electrospray ionization mass spectrometry. addition lipidomic analysis, fatty acid was analysed gas chromatography/mass We...

This study was undertaken to characterize functions of the outer membrane protein OmpW, which potentially contributes development colistin‐ and imipenem‐resistance in Acinetobacter baumannii . Reconstitution OmpW artificial lipid bilayers showed that it forms small channels (23 pS 1 m KCl) markedly interacts with iron colistin, but not imipenem. In vivo, 55 Fe uptake assays comparing behaviours Δ ompW mutant wild‐type strains confirmed a role for A. homeostasis. However, loss expression did...

Protein lysine acetylation is a reversible and highly regulated post‐translational modification with the well demonstrated physiological relevance in eukaryotes. Recently, its important role regulation of metabolic processes bacteria was highlighted. Here, we reported acetylproteome Pseudomonas aeruginosa using proteomic approach. We identified 430 unique peptides corresponding to 320 acetylated proteins. In addition proteins involved various pathways, several enzymes contributing...