A5028328541- Estrogen and related hormone effects
- Sperm and Testicular Function
- Cancer, Lipids, and Metabolism
- Hormonal Regulation and Hypertension
- Adrenal and Paraganglionic Tumors
- Reproductive Biology and Fertility
- Prostate Cancer Treatment and Research
- Cancer, Hypoxia, and Metabolism
- Hormonal and reproductive studies
- Testicular diseases and treatments
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Photopolymerization techniques and applications
- Phytochemicals and Antioxidant Activities
- Hypothalamic control of reproductive hormones
- Cholesterol and Lipid Metabolism
- Antioxidant Activity and Oxidative Stress
- Effects and risks of endocrine disrupting chemicals
- Adipose Tissue and Metabolism
- Chemical Synthesis and Reactions
- Ovarian function and disorders
- Adipokines, Inflammation, and Metabolic Diseases
- Regulation of Appetite and Obesity
- Selenium in Biological Systems
- Sexual Differentiation and Disorders
- Reproductive Physiology in Livestock
University of Calabria
2016-2025
Matrix Research (United States)
2019
Azienda Ospedaliera di Cosenza
2018
Institute of Cell Biology and Neurobiology
2001
Abstract Estrogens play a crucial role in the development of ovarian tumors; however, signal transduction pathways involved hormone action are still poorly defined. The orphan G protein–coupled receptor 30 (GPR30) mediates nongenomic signaling 17β-estradiol (E2) variety estrogen-sensitive cancer cells through activation epidermal growth factor (EGFR) pathway. Whether estrogen α (ERα) also contributes to GPR30/EGFR is less understood. Here, we show that, ERα-positive BG-1 cells, both E2 and...
The higher incidence of thyroid carcinoma (TC) in women during reproductive years compared with men and the increased risk associated therapeutic use estrogens have suggested a pathogenetic role exerted by these steroids development TC. In present study, we evaluated potential 17β-estradiol (E2), genistein (G), 4-hydroxyta-moxifen (OHT) to regulate expression diverse estrogen target genes proliferation human WRO, FRO, ARO cells, which were used as model system. We ascertained that cells are...
Leptin is a hormone with multiple biological actions, produced predominantly by adipose tissue. In humans, plasma levels correlate total body fat, and high concentrations occur in obese women. Among its functions, leptin able to stimulate normal tumor cell growth. We demonstrated that induces aromatase activity MCF-7 cells evidencing important role enhancing situ estradiol production promoting estrogen-dependent breast cancer progression. Estrogen receptor alpha (ERalpha), which plays an...
Human and animal models have evidenced how estrogen insufficiency is associated with abnormal spermatogenesis male infertility. We previously demonstrated that estradiol able to influence both capacitation acrosome reaction in human ejaculated spermatozoa. It remains be elucidated whether the biochemical changes induced by estradiol, a rapid nongenomic way, are mediated single receptor (ER) or two ER subtypes, ERα ERβ. In present study, we first concomitant expression of ERβ By RT-PCR...
Inflammation represents an adaptive response generated by injuries or harmful stimuli. Natural remedies represent interesting alternative to traditional therapies, involving several biochemical pathways. Besides, the valorization of agrochemical wastes nowadays seems be a feasible way reduce health spending and improve accessibility at bioactive natural compounds. In this context, chemical composition three Glycyrrhiza glabra L. (licorice) leaf extracts, obtained through maceration...
Antioxidant capacity of seminal plasma was evaluated in 120 semen samples subdivided into asthenozoospermic and oligoasthenozoospermic specimens with normal consistency hyperviscosity. Semen (n = 25) from normozoospermic donors were used as a control group. Scavenger antioxidant reactive oxygen species by superoxide dismutase catalase activity measurements, whereas the chain-breaking efficiency detected total status assessment. In viscosity, unaltered enzymatic nonenzymatic revealed...
Abstract Background Estrogen signaling is mediated by estrogen receptor beta isoforms in normal and neoplastic human testes. Recently, a G-protein-coupled-receptor (GPER) has been suggested as being involved rapid responses to estrogens different tumor cells. Methods This study investigated the GPER expression paraffin-embedded samples from non testes (sex-cord stromal germ cell tumors) immunohistochemical Western Blot analyses. Results In control testes, positive immunoreactivity was...
Abstract Cancer-associated fibroblasts (CAFs), the principal components of tumor stroma, play a central role in cancer development and progression. As an important regulator crosstalk between breast cells CAFs, cytokine leptin has been associated to carcinogenesis. The nuclear Farnesoid X Receptor-(FXR) seems exert oncosuppressive different tumors, including cancer. Herein, we demonstrated, for first time, that synthetic FXR agonist GW4064, inhibiting signaling, affects tumor-promoting...
The healing of skin wounds and particularly chronic wounds, such as diabetic foot ulcers, is still a clinical emergency. Despite the many therapeutic tools that are available so far, none seems to be really effective safe. In this context, we highlighted renewed wound activity honey, viscous aromatic sweet food, by way in vitro wound-healing assays, using HaCaT cell line. Specifically, investigated five monofloral or multifloral honeys from different Calabrian provinces them extracted (by...
Cardiac lipotoxicity is an important contributor to cardiovascular complications during obesity. Given the fundamental role of endoplasmic reticulum (ER)-resident Selenoprotein T (SELENOT) for cardiomyocyte differentiation and protection regulation glucose metabolism, we took advantage a small peptide (PSELT), derived from SELENOT redox-active motif, uncover mechanisms through which PSELT could protect cardiomyocytes against lipotoxicity. To this aim, modeled cardiac by exposing H9c2...
Previous epidemiological reports have suggested that red wine intake is associated with beneficial health effects due to the ability of certain phytochemical components exert estrogen-like activity. It has been also documented estrogens induce proliferation hormone-dependent breast cancer cells by binding and transactivating estrogen receptor (ER) α, which in turn interacts responsive DNA sequences located within promoter region target genes. In order provide further insight into positive...
Several studies have recently investigated the role of leptin, adipocyte-secreted hormone, in growth and reproduction rodents, humans, domestic animals. The present study was designed to explore expression leptin its receptor pig spermatozoa. Successful Western blot evidenced a 16 kDa band for six isoforms, ranging from 120 40 kDa, receptor. Both were interestingly located at sperm acrosomal level, suggesting their involvement oocyte fertilization events. In fact, both capacitation indexes...
Psoralens (5-MOP and 8-MOP), a class of naturally occurring compounds, in combination with ultraviolect light are potent modulators epidermal cell growth differentiation. For long time, photo-chemotherapy has been used the treatment psoriasis where it can reduce number cycling keratinocytes decrease IGF-1 receptors. However, molecular mechanism PUVA therapy remains unclear. In this study, we have evaluated, for first MCF-7 SKBR-3 breast cancer cells effects 5-MOP (Bergapten), independently...
The mechanisms by which varicocele affects fertility remain undetermined. Vitamin A (all‐ trans retinoic acid [ATRA]) is required for and normal spermatogenesis; however, the driving its action are not defined yet. Previously, we demonstrated in sperm a reduced RARα expression that ATRA influence performance. To further define vitamin significance male gamete physiopathology of varicocele, tested first time on human metabolism antioxidant defense systems. Evaluating triglycerides content...
// Ivan Casaburi 1, * , Paola Avena Arianna De Luca Adele Chimento 1 Rosa Sirianni Rocco Malivindi Vittoria Rago Marco Fiorillo Francesco Domanico Carmela Campana Anna Rita Cappello Federica Sotgia 2 Michael P. Lisanti Vincenzo Pezzi Department of Pharmacy, Health and Nutritional Sciences, University Calabria, Italy The Breakthrough Breast Cancer Research Unit the Manchester Centre for Cellular Metabolism, Institute Manchester, UK These authors have contributed equally to this work...
Abstract Background The incretin hormone glucagon‐like peptide‐l (GLP‐1) is an important regulator of post‐prandial insulin secretion, acting through a G protein‐coupled cell surface receptor (GLP‐1R). In addition to its expression in pancreatic β‐cells, several studies suggested that GLP‐1R located extra‐pancreatic tissues. Objectives this study, we examined for the first time testicular distribution GLP‐1R, both normal human and neoplastic tissues as well rodent testis lines. Methods...
Abstract Cytochrome P450 aromatase is a terminal enzyme that catalyses the conversion of androgens into oestrogens. This study investigated immunohistochemical localization in human efferent ductules and proximal ductus epididymis using mouse anti‐human monoclonal P450arom IgG as primary antibody goat anti‐mouse biotinylated secondary antibody. A strong immunoreaction was observed epithelial cell cytoplasm both ductuli efferentes epididymis, whereas smooth muscle cells were immunonegative...
Androgens and estrogens are crucial for mammalian sperm differentiation but their role in biology of mature male gamete is not still defined. The expression proteins involved the biosynthesis action these steroid hormones has been demonstrated human spermatozoa, very few data have reported from non species. purpose current study was to investigate aromatase (P450arom), estrogen (ERalpha/ERbeta) androgen (AR) receptors ejaculated spermatozoa pig.The immunfluorescence experiments were carried...
Oxazolidinones are antibiotics that inhibit protein synthesis by binding the 50S ribosomal subunit. Recently, numerous worldwide researches focused on their properties and possible involvement in cancer therapy have been conducted. Here, we evaluated vitro antiproliferative activity of some 5-(carbamoylmethylene)-oxazolidin-2-ones MCF-7 HeLa cells. The tested compounds displayed a wide range cytotoxicity these cell lines, measured MTT assay, exhibiting no non-tumorigenic MCF-10A Among nine...