A5068814974- Photosynthetic Processes and Mechanisms
- Medicinal Plants and Neuroprotection
- Plant Stress Responses and Tolerance
- Cellular transport and secretion
- Nanoparticles: synthesis and applications
- Plant-Microbe Interactions and Immunity
- Ginger and Zingiberaceae research
- Protein purification and stability
- Phytochemicals and Antioxidant Activities
- Medicinal Plants and Bioactive Compounds
- Lipid Membrane Structure and Behavior
- Drug-Induced Hepatotoxicity and Protection
- Microfluidic and Capillary Electrophoresis Applications
- Plant Disease Resistance and Genetics
- Plant Virus Research Studies
- Natural Antidiabetic Agents Studies
- Plant-based Medicinal Research
- Plant nutrient uptake and metabolism
- Lipid metabolism and biosynthesis
- Analytical Chemistry and Chromatography
- Plant tissue culture and regeneration
- RNA and protein synthesis mechanisms
- Heavy Metals in Plants
- Gut microbiota and health
- Endoplasmic Reticulum Stress and Disease
University of Management and Technology
2023-2024
University of Malakand
2014-2023
University of Balochistan
2023
University of Gothenburg
2010-2014
Pakistan Army
2000
Health Information Management
2000
World Health Organization - Afghanistan
2000
Nanotechnology is perhaps the most widely explored scientific domain in current era. With advent of NPs, revolutionary changes have been observed various disciplines. Among ZnO-NPs are center contemplation owing to their biocompatible nature. These nanoparticles prepared using a number techniques; however, biological methods among popular synthesis approaches. The research therefore reports phyto-fabrication mediated by Delphinium uncinatum root extract. resulting NPs were subjected standard...
The molecular chaperone ClpC/Hsp93 is essential for chloroplast function in vascular plants. ClpC has long been held to act both independently and as the regulatory partner ATP-dependent Clp protease, yet this many other important characteristics remain unclear. In study, we reveal that of two near-identical paralogs (ClpC1 ClpC2) Arabidopsis chloroplasts, along with closely related ClpD, it ClpC1 most abundant throughout leaf maturation. An unexpectedly large proportion proteins (30% total...
Green synthesis of nanoparticles is becoming a method choice for biological research due to its environmentally benign outcomes, stability and ease synthesis. In this study, silver (AgNPs) were synthesized using stem (S-AgNPs), root (R-AgNPs) mixture (RS-AgNPs) Delphinium uncinatum. The characterized by standardized techniques evaluated their antioxidant, enzyme inhibition, cytotoxic antimicrobial potentials. AgNPs exhibited efficient antioxidant activities considerable inhibition potential...
Thylakoid biogenesis is a crucial step for plant development involving the combined action of many cellular actors. CPSAR1 shown here to be required normal organization mature thylakoid stacks, and ultimately embryo development. chloroplast protein that has dual localization in stroma inner envelope membrane, according microscopy studies subfractionation analysis. close Obg nucleotide binding subfamily displays GTPase activity, as demonstrated by vitro assays. Disruption gene via T-DNA...
Proteins and lipids are known to be transported targeted cytosolic compartments in vesicles. A similar system chloroplasts is suggested transfer from the inner envelope thylakoids. However, little about both possible cargo proteins required build a functional vesicle transport chloroplasts. few components have been suggested, but only one (CPSAR1) has verified location chloroplast This protein localized donor membrane (envelope) vesicles, not target (thylakoids) suggesting it plays role...
Dynamin-like proteins (DLPs) are a family of membrane-active with low sequence identity. The operate in different organelles eukaryotic cells, where they trigger vesicle formation, membrane fusion, or organelle division. As discussed here, representatives this protein have also been identified chloroplasts and DLPs very common cyanobacteria. Since cyanobacteria chloroplasts, an bacterial origin, similar internal systems, we suggest that involved dynamics chloroplasts. Here, discuss the...
The Tic22 protein was previously identified in pea as a putative component of the chloroplast import apparatus. It is peripheral inner envelope membrane, residing intermembrane space. In Arabidopsis, there are two homologues, termed atTic22-III and atTic22-IV, both which predicted to localize chloroplasts. These proteins defined clades that conserved all land plants, appear have evolved at similar rates since their separation >400 million years ago, suggesting functional conservation....
Stress associated proteins (SAPs) in plants have a key role providing tolerance to multiple abiotic stresses. SAP gene family Solanum tuberosum has not been fully studied before. This study identified 17 StSAP genes S. which code for A20/AN1 zinc-finger proteins. All the were distributed on ten different chromosomes and six segmental duplication events identified. The SAPs its orthologs Arabidopsis thaliana classified into groups through phylogenetic analysis. Introns across four genes....
Abstract Nucleotide-binding site ( NBS ) domain genes are one of the superfamily resistance involved in plant responses to pathogens. The current study identified 12,820 NBS-domain-containing across 34 species covering from mosses monocots and dicots. These classified into 168 classes with several novel architecture patterns encompassing significant diversity among species. Several classical NBS, NBS-LRR, TIR-NBS, TIR-NBS-LRR , etc.) species-specific structural TIR-NBS-TIR-Cupin_1-Cupin_1,...
Plant growth, reproduction and yields are severely damaged under adverse environmental stresses. These stresses can be either biotic or abiotic, many stress related proteins expressed in response to these Among dehydrins reported have a role primarily the abiotic Dehydrins very diverse uniform annotation system is needed for their functional characterization future research.The aim of present work identify, classify analyze expression dehydrin different selected plant species by using...
Vesicle transport occurs in the cytosol through COPI, COPII and a clathrin coated vesicle system for of lipids proteins to different subcellular compartments. All three systems consist several protein components maintain functional transport. In chloroplasts photosynthesis takes place thylakoids. Thylakoids contain large amount but none these are produced there. Transport from envelope membrane where they aqueous stroma before being directed Nuclear encoded use distinct pathways entering...
Several toxic compounds are known to induce apoptosis in mammalian cell lines. The human neuroblastoma cells (SH-SY5Y) were exposed the phosphatase inhibiting toxin okadaic acid (OA) or hydrogen peroxide (H2O2) as well generate reactive oxygen species (ROS). Mitoxantrone (MXT) was used a positive control for apoptosis. SH-SY5Y transfected with eukaryotic expression plasmid pHyPer-dMito encoding mitochondrial-targeted fluorescent pHyPer-dCito cytoplasmic-targeted sensor (HyPer). ERp57, also...
The analgesic, antidiarrheal, and neuro-pharmacological potentials of Medicago denticulata leaves extract were screened in animal models. Potential analgesic response was noted (*P < 0.05, **P 0.01, ***P 0.001) formalin, acetic acid heat-induced pain models a dose-dependent manner. Maximum activity by means writhing inhibition documented for at 300 mg/kg that found to be 71.79% (17.43 ± 1.31). In first phase, the dose 150 showed reduced 54.18% (18.39 1.67) 62.90% (14.89 1.56), respectively....
Abstract This part of work was done to explore the basic understanding adsorption chromatography by determining interaction selected model proteins ( n = 5) monolithic chromatographic materials, with varying densities butyl and phenyl ligands. Surface energetics approach applied study behavior. The physicochemical properties materials were explored contact angle zeta potential values. These values used protein monolith under various operating conditions. allowed calculation energy as a...
Aqueous two-phase system (ATPS) is an efficient, cost effective, fast, simple and ecofriendly method for the recovery of biomolecules. In present study, ATPS composed polyethylene glycol ammonium sulphate (NH4)2SO4 was used partial purification proteases from microbial source. The effects different parameters such as molecular weight PEG (4000, 6000 10000), concentration (15, 17.5 20 %) (7.5, 8.3, 9.1 9.9 on partitioning behavior at room temperature were investigated. Generally, increasing...
Abstract Hydrophobic interaction chromatography, an important and effective purification strategy, is generally used for the of variety biomolecules. A basic understanding protein behavior required to effectively separate these colloidal type extended Derjaguin, Landau, Verwey, Overbeek calculations were utilized study interactions model proteins commercially available hydrophobic chromatographic materials that is, Toyopearl Phenyl 650C Butyl 650C. Physicochemical properties selected...