Bacteria in rumen play pivotal roles the digestion of nutrients to support energy for host. In this study, metagenomic deep sequencing bacterial metagenome extracted from goats' generated 48.66 GB data with 3,411,867 contigs and 5,367,270 genes. The genes were mainly functionally annotated by Kyoto Encyclopedia Genes Genomes (KEGG) Carbohydrate-Active enZYmes (CAZy), HMMER database, taxonomically classified MEGAN. As a result, 65,554 encoding 30 enzymes/proteins related lignocellulose...

The degQ gene of Bacillus subtilis (natto), encoding a small peptide 46 amino acids, is essential for the synthesis extracellular poly-gamma-glutamate (γPGA). To elucidate role DegQ in γPGA synthesis, we knocked out (natto) and screened suppressor mutations that restored absence DegQ. Suppressor were found degS, receptor kinase DegS-DegU two-component system. Recombinant DegS-His(6) mutant proteins expressed Escherichia coli cells subjected to an vitro phosphorylation assay. Compared with...

An industrial strain of Bacillus subtilis (natto) was used to produce poly-gamma-dl-glutamate (γPGA), a polymer dl-glutamate linked by γ-peptide bond. In spite efforts improve γPGA production modifying the medium, little attention has been paid expression synthetase gene. this study, we investigated synthetic gene and product under various conditions with LacZ-fusion (pgsB-lacZ). The 5′ upstream regulatory region pgsB also constructing deletion mutations lacZ-fusion. pgsB-lacZ clearly...

In a previous study, analysis of Illumina sequenced metagenomic DNA data bacteria in Vietnamese goats' rumen showed high diversity putative lignocellulolytic genes. this taxonomy speculation microbial community and population the was conducted to elucidate role bacterial structure for effective degradation plant materials.The had been subjected into Basic Local Alignment Search Tool (BLASTX) algorithm National Center Biotechnology Information non-redundant sequence database. Here BLASTX hits...

Abstract Background The discovery of the autotransporter family has provided a mechanism for surface expression proteins in laboratory strains Escherichia coli . We have previously reported use AIDA-I autotransport system to express Salmonella enterica serovar Enteritidis SefA and H:gm. protein was successfully exposed medium, but orientation H:gm outer membrane could not be determined due proteolytic cleavage N-terminal detection-tag. goal present work therefore construct vector containing...

Termites' digestive systems, particularly in lower termites with the presence of protozoa, are unique ecological niches that shelter a diverse microbiota variety functions for host and environment. In 2012, metagenomic DNA (5.4 Gb) prokaryotes freely live gut termite Coptotermes gestroi were sequenced. A total 125,431 genes predicted analyzed order to mine lignocellulolytic genes. however, overall picture structure, diversity, function prokaryotic was not investigated. present study, these...

The scarcity of enzymes having an optimal activity in lignocellulose deconstruction is obstacle for industrial-scale conversion cellulosic biomass into biofuels. With the aim mining novel lignocellulolytic enzymes, a ~9 Gb metagenome bacteria Vietnamese native goats' rumen was sequenced by Illumina platform. From data, 821 ORFs encoding carbohydrate esterases (CEs) and polysaccharide lyases (PLs) serving pre-treatment, 816 11 glycoside hydrolase families (GHs) cellulases, 2252 22 GHs...

Objective: Fibronectin 3 (FN3) and immunoglobulin like modules (Ig) are usually collocated beside modular cellulase catalytic domains. However, very few researches have investigated the role of these modules. In a previous study, we sequenced analyzed bacterial metagenomic DNA in Vietnamese goats' rumen found that cellulase-producing bacteria families were dominant. this properties cellulases FN3 unique endoglucanase belonging to glycosyl hydorlase (GH) family 5 determined.Methods: Based on...

We aimed to investigate the microbial diversity, mine lignocellulose-degrading enzymes/proteins, and analyze domain structures of mined enzymes/proteins in humus samples collected from Cuc Phuong National Park, Vietnam. Using a high-throughput Illumina sequencer, 52 Gbs DNA were assembled 2,611,883 contigs, which 4,104,872 open reading frames (ORFs) identified. Among total microbiome analyzed, bacteria occupied 99.69%; five ubiquitous bacterial phyla included Proteobacteria, Bacteroidetes,...

Salmonella enterica serovar Enteritidis (SE) is one of the most potent pathogenic serotypes causing food-borne diseases in humans. We have previously reported use β-autotransporter AIDA-I to express flagellar protein H:gm and SE serotype-specific fimbrial SefA at surface E. coli as live bacterial vaccine vehicles. While was successfully displayed cell surface, virtually no full-length exposed medium due extensive proteolytic cleavage N-terminal region. In present study, we addressed this...

In Vietnam, diarrhea, especially persistent is one of the most common diseases in children, while a significant proportion cases are negative with pathogens; thus, there an urgent need to understand gut bacterial dysbiosis. this study, bacteria fecal samples five healthy and ten diarrheal children were separated from other residues, then adopted extract their metagenomic DNA for evaluating diversity based on V3 V6–V8 regions 16S rRNA gene by PCR-RFLP PCR-DGGE. As result, DNAs high quality,...

-glucopyranoside, IL-12 p40, IL-6Inflammatory responses, initiated by the invasion of patho-gens or tissue injury caused free radicals, are a seriesof vascular and cellular reactions. Some important chemicalmediators inflammation interleukin (IL)-1, -6, -12,and tumor necrosis factor-α, prostaglandins, etc… IL-6 haspro- anti-inflammatory properties. is involved notonly in activation immune system but also inregenerative process as well regulation meta-bolism.

Background: Enterocin-P of Enterococcus faecium P13 (EntP) is great interest as a food preservative and medicine due to its non-toxicity broad antimicrobial spectrum at various pH, well excellent thermal stability.However, recombinant production EntP still in laboratory because low productivity complex purification process.Objectives: In this study, we aimed develop efficient methods for high-level expression convenient the EntP.Materials Methods: An artificially synthesized gene (entP) 132...

Salmonella have many advantages as a vaccine delivery vector, they are easy to produce, administer (orally), and able elicit humoral immunity which induces serum secretory IgA antibody. In addition, induce robust cell-mediated immune responses such cytotoxic memory T lymphocytes. Moreover, when is being used against both the heterologous antigen will be induced, providing protection infection by pathogen. this study, STM-1, an attenuated strain was developed at RMIT licensed for prevention...

Blumea lanceolaria (Roxb.) Druce, a flowering plant, is used for treating cancer and inflammatory diseases. In this study, we determined the chemical composition of EOs extracted from leaves (LBEO), stem (SBEO), roots (RBEO) B. analyzed their anti-inflammation potential. Overall, 30 compounds representing 99.12%, 98.44%, 96.89% total EO constituents leaves, stem, roots, respectively, were identified using GC-MS. ELISA, Western blotting, qRT-PCR studies showed that LBEO, SBEO, RBEO inhibited...

Background White-rot fungi and bacteria communities are unique ecosystems with different types of symbiotic interactions occurring during wood decomposition, such as cooperation, mutualism, nutritional competition, antagonism. The role chitin-active lytic polysaccharide monooxygenases (LPMOs) in these is the subject this study. Method In study, bioinformatics tools were used to analyze sequence structure putative LPMOs mined by hidden Markov model (HMM) profiles from bacterial metagenomic...

Acute diarrhea is a common disease in children under 5 years old and can develop into persistent diarrhea, greatly affecting the children's health. Although advanced techniques had been used to diagnose detect pathogens hospitals, however, 40% of cases are negative for pathogens. In this study, investigate dominant bacteria stool samples three persistent-diarrheal with unidentified pathogenic agents, V3 V68 regions 16S rRNA gene were amplified from fecal bacterial metagenomic DNA, separated...

The HA1 genes from influenza A strains A/Puerto Rico/8/1934 H1N1 (A/PR/8/34) and A/Hatay/2004 H5N1 were each cloned in Pichia pastoris vectors the correct reading frame with yeast α-factor secretion signal C-terminus His-tag, resulting simple, fast purification of expressed H1HA1 H5HA1 protein culture medium. Mice vaccinated purified proteins showed robust T cell, anti-HA1 IgG responses developed a high antibody response for hemagglutination inhibition (HI) at titer 7.6 log2. Chickens dose...