Aim: Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), a pernicious viral disease, causes distress responsible for mortality and morbidity worldwide. To screen different immunomodulatory medicinal compounds to unravel their interaction with SARS-CoV-2 proteins. Materials & methods: A library of antiviral capability were analyzed against SARS proteases, spike protein nonstructural proteins (NSP-9, 15) using Autodock vina. Results: Out more than 300 compounds, only six compounds:...

Constitutive photomorphogenic 1 (COP1), is an E3 ubiquitin ligase that plays a role in the regulation of various cellular processes including cell growth, differentiation, and survival mammals. In certain conditions such as overexpression or loss function, COP1 acts either oncogenic protein tumor suppressor by targeting specific proteins for ubiquitination-mediated degradation. However, precise has not been well studied primary articular chondrocytes. this study, we investigated chondrocyte...

Inositol-requiring enzyme-1 (IRE1) is the master regulator of unfolded protein response pathway, associated with endoplasmic reticulum (ER) in sensing and regulating cell stress. The activity IRE1 highly explored well-characterized cancer other cells. However, molecular mechanism chondrocytes poorly understood. present study effect on regarding its chondrogenic gene expression correlation different cellular pathways behavior. Chondrocytes transfected cDNA reduced type II collagen, disrupting...

To evaluate the inhibitory interaction of thymohydroquinone against blood-brain barrier (BBB)-associated neuropsychiatric and neurodegenerative disorders.An elaborated in silico study was designed to with BBB-disrupting proteins highlight its pharmacokinetic safety attributes.Thymohydroquinone demonstrated stable protein active site Ki (inhibition constant) ranges (2.71 mM-736.15 μM), binding energy (-4.3 5.6 Kcal/mol), ligand efficiency (-0.36 0.42 Kcal/mol) root mean square deviation value...

Aim: The present study was performed to determine the inhibitory interaction of fever-relieving medicines with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) essential proteins. Materials & methods: Structure-based drug repositioning using PYRX 0.9 and these drugs were directed toward predicted active site SARS-CoV-2 spike glycoprotein receptor-binding domain, main protease RNA-dependent RNA polymerase. Results: Results showed that acetaminophen naproxen have considerable...

Some bioactive derivatives of indeno[1,2-c]pyrazolones were synthesized through the reaction phenylhydrazine, different aldehydes and indan-1,2,3-trione at room temperature in acetonitrile. Analytical spectroscopic studies have confirmed structural characteristics compounds. In addition, target compounds screened for in-vitro antiproliferative properties against B16F10 melanoma cancer cell lines by standard MTT assay. The effect on inflammatory marker cyclooxygenase 2 matrix...

Among sarcomas, Ewing sarcoma (EWS) is characterized as a highly malignant type of bone tumor caused by the fusion EWS RNA Binding Protein-1 (EWSR1)/ Friend leukemia integration 1 (FLI1) genes. The product gene gives rise to EWSR1/FLI1 which activates activity Eyes absent homolog 3 (EYA3) causes growth and angiogenesis. EYA3 now considered therapeutic drug target for . study was designed gather potential inhibitors using medicinal compounds.In this study, we have obtained list compounds from...

Background: Fibroleukin-2 protein (FGL2) causes redevelopment of brain tumors. Inhibition these proteins has shown to improve glioblastoma prognosis and treatment efficacy. Aim: The current study gathered recently exploited natural compounds that suppress proliferation in vitro, tested against FGL2 protein. Method: Twenty-five were explored through a virtual screening platform. Results: Three (betanine, hesperetin ovatodiolide) hit the active site FGL2. Furthermore, influence was also...

Aim: Peptide/protein-based inhalers are excessively used to treat respiratory disorders. The molecular docking was performed for these including human neutralizing S230 light chain-antibody (monoclonal antibodies [mAbs]), alpha-1-antitrypsin (AAT), short-palate-lung and nasal-epithelial clone-1-derived peptides (SPLUNC1) dornase-alfa (DA) against spike glycoprotein of severe acute syndrome coronavirus 2 (SARS-CoV-2) assess their inhibitory activity. Materials & methods: HawkDock dock...

Chondrosarcoma is the third most common cartilaginous bone tumour that insusceptible to radio- and chemotherapy it inclined metastasis. These resistant qualities are facilitated by mutant variants of isocitrate dehydrogenases (IDH) 1-2 enzyme. enzymes promote oncogenesis chondrocytes changing their epigenetic wardrobe leading formation. Presently, there lack drugs available be exploited as a remedy for this disease. On other hand, majority chemotherapeutic induce cytotoxicity in cancer cells...

The world is under siege from a global pandemic caused by novel class of coronaviruses called severe acute respiratory syndrome coronavirus-2 (SARS CoV-2). These viruses cause illness leading to death. Molecular studies reveal that SARS CoV-2 proteases are involved in the processing viral polyproteins. This study was conducted obtain antiviral agents for proteases. An extensive library medicinal compounds scrutinized determine probable interaction with both main and 3-chymotrypsin like Six...

Background: DNA methyltransferases (DNMTs) are a group of epigenetic enzymes implicated in regulating gene expression actively dividing cells. Among these enzymes, DNMT1 plays leading role causing increased methylation tumor suppressors and other genes cancer This event disrupts the cell cycle genes, allowing an uninterrupted proliferation cells, stimulating inhibition degradation proteins aberrant transcription activation. Cytosine analog drugs have been shown to reduce but provoke...

Due of the intricacy their illness and possibility complications, patients with acute pancreatitis may need to stay in hospital longer or shorter, approach used. Objective: To compare treatment moderate fluid resuscitation aggressive as well duration stay. Methods: It was cross-sectional study conducted Department Medicine Muhammad Teaching Hospital Peshawar, six months, from July 2023 December 2023. A total number participants (N=170) this study. The age >16 years included both male...