The major unexplained phenomenon in fibrotic conditions is an increase replicating fibroblasts. In this report we present evidence that oxygen free radicals can both stimulate and inhibit proliferation of cultured human fibroblasts, fibroblasts themselves release superoxide (O2.-) radicals. Fibroblasts released O2.- concentrations which stimulated proliferation, a finding confirmed by dose-dependent inhibition radical scavengers. Oxygen host agents may thus provide very fast, specific...

The effects of retinol (vitamin A) and retinoic acid on primary cultures isolated chicken osteoclasts have been studied. experiments were performed to establish the direct actions these two agents organization cytoskeletal structures, phosphatase contents, bone resorption activities cells. results showed that by treating with or acid, from 10(-8) 10(-5) M, there dose-related responses osteoclasts. Adhesion substratum was stimulated increasing number cells exhibiting specialized dot-like...

The concentration of substrate expressed as hypoxanthine capable reacting with xanthine oxidase to release superoxide free radicals (O2-) was measured in control and Dupuytren9s contracture palmar fascia. In fascia the six times that greatest "nodular" areas. Xanthine activity also detected These results suggest a greater potential for hypoxanthine-xanthine generated oxygen radical formation than Production may be an important factor pathogenesis contracture. benefit allopurinol management...

1. Weanling rabbits were made rachitic either by a vitamin D-deficient diet or parenteral administration of ethane 1-hydroxy-1,1-diphosphonate (EHDP) in amounts sufficient other species to block the formation 1,25-dihydroxycholecalciferol [1,25-(OH)2D3]. 2. The uptake calcium into isolated sarcoplasmic reticulum from mixed striated quadriceps muscle, and amount troponin C (the calcium-binding component complex) relation proteins same measured. 3. In muscle animals dietary deficiency D, rate...

Abstract Background Research has shown that the impacts of COVID-19 pandemic stretch beyond solely physical health. This study uses newly developed Epidemic-Pandemic Impacts Inventory (EPII), a 92-item tool designed to quantify impact across several personal and social domains. People living with HIV (PLWH) have an increased morbidity mortality associated infection are overall more vulnerable change. The current EPII compare pandemic-related experiences PLWH those without in New Orleans....

In Dupuytren’s contracture there is an increase in the ratio of type III to I collagen. The objective this study was determine if fibroblasts from patients with have intrinsic aberration collagen production or whether local factors govern changes contracture. Using a new micro-method, we found that cultured palmar fascia affected by produced similar derived age- and sex-matched carpal tunnel syndrome. Furthermore, could be reproduced all cell lines increasing fibroblast density. At high...

Cellular mechanisms accounting for the osteolysis of rheumatoid erosions are poorly understood. Cells were isolated and characterised from synovium 16 patients with arthritis (RA) four osteoarthritis their ability to resorb bone was assessed using a scanning electron microscope resorption assay. Macrophages major cell type RA. These produced extensive roughening surface without pit formation. This low grade not affected by systemic (calcitonin, parathyroid hormone, 1,25-dihydroxyvitamin D3)...

SUMMARY Isolated rat liver, when perfused with medium containing bovine growth homone produced somatomedin-like activity (liver somatomedin). Liver somatomedin is useful in studies of the hormonal control cartilage plate vitro , since unlike serum it not contaminated other hormones or factors (apart from hormone). Chondrocytes isolated various regions responded differently to liver somatomedin; proliferative chondrocytes, like those articular cartilage, showed increased [ 3 H]thymidine...

Rat prolactin at a concentration of 50 ng/ml perfusion medium stimulated the production somatomedin-like activity (SLA) from perfused liver normal rats. The effect was demonstrable in perfusions performed 11.00 h which rat caused mean (+/- S.E.M.) increase uptake [35S]sulphate into costal cartilage vitro 64 +/- 14% comparison with controls, but 15.00 no observed. No or on hypophysectomized detectable h. Hypophysectomized and sham-operated rats were given five intravenous injections microgram...

Plasma somatomedin activity enhanced the incorporation of [ 3 H]thymidine into chondrocytes isolated from human foetal cartilage during weeks 13–21 gestation. Human growth hormone (0·1–20 μu./ml), placental lactogen (0·1–5 μg/ml) and insulin (0·25– 10 μu./ml) had no direct effects on synthesis DNA in these chondrocytes, although at concentrations 2·5–100 mu./ml increased by up to 400%.

The clinical features of 62 patients with the diagnosis osteogenesis imperfecta, a generalized connective tissue disorder, have been reviewed. structural polymeric collagen skin has examined by biochemical and histological methods in 21 these two further osteogensis imperfecta who died soon after birth. results provide evidence heterogeneity within this group patients. Three main types disorder are recognizable: those dominantly inherited mild bone disease, blue sclerae low amounts collagen;...

Research Article| June 01 1966 Phosphatidylglycerylglucosamine P. J. R. Pizackerley; Pizackerley Search for other works by this author on: This Site PubMed Google Scholar C. Macdougall; Macdougall M. O. Francis Biochem J (1966) 99 (3): 21C–22C. https://doi.org/10.1042/bj0990021C Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Facebook Twitter LinkedIn MailTo Cite Get Permissions Citation Pizackerley, Macdougall, Francis;...