A5000546524- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Electrospun Nanofibers in Biomedical Applications
- Bone Tissue Engineering Materials
- Tissue Engineering and Regenerative Medicine
- Tendon Structure and Treatment
- Mesenchymal stem cell research
- Biomedical and Engineering Education
- Periodontal Regeneration and Treatments
- Osteoarthritis Treatment and Mechanisms
- Biomedical Ethics and Regulation
- Planarian Biology and Electrostimulation
- Supramolecular Self-Assembly in Materials
- Knee injuries and reconstruction techniques
- Cancer Cells and Metastasis
- Bone and Dental Protein Studies
- Health and Medical Research Impacts
- Laser Applications in Dentistry and Medicine
- Nanoparticles: synthesis and applications
- Angiogenesis and VEGF in Cancer
- Seed Germination and Physiology
- Additive Manufacturing and 3D Printing Technologies
- Wound Healing and Treatments
- Chemical Reactions and Isotopes
- Neuroscience and Neural Engineering
Ollscoil na Gaillimhe – University of Galway
2014-2023
Science Foundation Ireland
2016-2023
National University of Ireland
2016-2022
Universidade do Porto
2012
Modular tissue engineering is based on the cells' innate ability to create bottom-up supramolecular assemblies with efficiency and efficacy still unmatched by man-made devices. Although regenerative potential of such substitutes has been documented in preclinical clinical setting, prolonged culture time required develop an implantable device associated phenotypic drift and/or cell senescence. Herein, we demonstrate that macromolecular crowding significantly enhances extracellular matrix...
Background: Electro-spun scaffolds are utilized in a diverse spectrum of clinical targets, with an ever-increasing quantity work progressing to studies and commercialization. The limited number conformations which the can be fabricated hampers their wide acceptance practice. Materials & methods: Herein, we assessed single-strep fabrication process for predesigned electro-spun scaffold preparation ramifications introduction porosity (0, 30, 50, 70%) pore shape (circle, rhomboid, square) on...
Bottom-up bioengineering utilizes the inherent capacity of cells to build highly sophisticated structures with high levels biomimicry. Despite significant advancements in field, monodomain approaches require prolonged culture time develop an implantable device, usually associated cell phenotypic drift culture. Herein, we assessed simultaneous effect macromolecular crowding (MMC) and mechanical loading enhancing extracellular matrix (ECM) deposition while maintaining tenocyte (TC) phenotype...
Background: Cell culture media containing undefined animal-derived components and prolonged in vitro periods the absence of native extracellular matrix result phenotypic drift human bone marrow stromal cells (hBMSCs). Methods: Herein, we assessed whether animal component-free (ACF) or xeno-free (XF) formulations maintain hBMSC characteristics more effectively than foetal bovine serum (FBS)-based media. In addition, tissue-specific matrix, induced via macromolecular crowding (MMC) during...
During in vitro culture, bereft of their optimal tissue context, tenocytes lose phenotype and function. Considering that native milieu are exposed simultaneously to manifold signals, combination approaches (e.g. growth factor supplementation mechanical stimulation) continuously gaining pace control cell fate during expansion, albeit with limited success due the literally infinite number possible permutations. In this work, we assessed potential scalable potent physicochemical (substrate...
The nano-fibrous architecture of electro-spun meshes favours their use in biomedicine, but low mechanical properties prohibit wide clinical practice. Introduction porosity, essential tissue integration, decreases further integrity. Herein, we hypothesised that macro-porous with adequate can be fabricated through layering and subsequent compression. Two three layers poly-ε-caprolactone scaffolds were fabricated, compressed subsequently 30% circular porosity was introduced laser cutting....
Low in macro-porosity electro-spun scaffolds are often associated with foreign body response, whilst macro-porous have low mechanical integrity. Herein, compressed, and collagen (bovine Achilles tendon human recombinant) coated poly-ε-caprolactone were developed their biomechanical, vitro vivo properties assessed. Collagen coating, independently of the source, did not significantly affect biomechanical scaffolds. Although no significant difference cell viability was observed between groups,...
Tissue engineering by self-assembly hypothesises that optimal repair and regeneration can be achieved best using the cells’ inherent ability to create organs with proficiency still unmatched currently available scaffold fabrication technologies. However, prolonged culture time required develop an implantable device jeopardises clinical translation commercialisation of such techniques. Herein, we report macromolecular crowding, a biophysical in vitro microenvironment modulator, dramatically...
Due to their inherent plasticity, dermal fibroblasts hold great promise in regenerative medicine. Although biological signals have been well-established as potent regulators of fibroblast function, it is still unclear whether physiochemical cues can induce trans-differentiation. Herein, we evaluated the combined effect surface topography, substrate rigidity, collagen type I coating and macromolecular crowding human cultures. Our data indicate that tissue culture plastic increased cell...