Exercise-induced oxidative stress is instrumental in achieving the health benefits from regular exercise. Therefore, inappropriate use of fruit-derived products (commonly applied as prophalytic antioxidants) may counteract positive effects Using human exercise and cellular models we found that 1) blackcurrant supplementation suppressed exercise-induced stress, e.g., plasma carbonyls (0.9 ± 0.1 vs. 0.6 nmol/mg protein, placebo blackcurrant), 2) preincubation THP-1 cells with an...

The ribosome stalk is essential for recruitment of translation factors. In yeast, P0 and Rpl12 correspond to bacterial L10 L11 form the base mature ribosomes, whereas Mrt4 a nuclear paralogue P0. this study, we show that dual-specificity phosphatase Yvh1 required release from pre-60S subunits. Deletion YVH1 leads persistence on subunits in cytoplasm. A mutation at protein–RNA interface bypasses requirement Yvh1. Pre-60S associated with contain but lack both These results suggest linear...

Developing a multiple functional wound dressing suitable for different stages of healing is important patients with special such as burn or decubital ulcers. In this study, poly (vinyl alcohol) (PVA), dextran, and chitosan are integrated to produce ideal where glutaraldehyde (GA) used the cross-linker. The result demonstrated that 6% PVA hydrogel 0.25% was found provide antimicrobial ability. PVA/chitosan combined 4% dextran utilizing GA cross-linking also presents high cell proliferation...

We previously showed that nuclear export of the large (60S) ribosomal subunit relies on Nmd3 in a Crm1-dependent manner. Recently general mRNA factor, Mtr2/Mex67 heterodimer, was shown to act as an receptor parallel with Crm1. These observations raise possibility 60S Saccharomyces cerevisiae requires multiple receptors. Here, we show characterized biogenesis Arx1, also acts for subunit. found deletion ARX1 synthetic lethal nmd3 and mtr2 mutants sick several nucleoporin mutants. Deletion led...

Cell migration is an essential process involved in the development and maintenance of multicellular organisms. Electric fields (EFs) are one many physical chemical factors known to affect cell migration, a phenomenon termed electrotaxis or galvanotaxis. In this paper, microfluidics chip was developed study cells under different electrical stimuli. This capable providing four strengths EFs combination with two chemicals via simple set agar salt bridges Ag/AgCl electrodes. NIH 3T3 fibroblasts...

Abstract Collective cell migration plays important roles in various physiological processes. To investigate this collective cellular movement, wound-healing assays have been developed. In these assays, a “wound” is created mechanically, chemically, optically, or electrically out of monolayer. Most are subject to drawbacks run-to-run variations wound size/shape and damages cells/substrate. Moreover, all cells cultured open, static (non-circulating) environments. study, we reported...

Reactive oxygen species (ROS) are known to be a key factor in the development of cancer, and many exogenous sources supposed related formation ROS. In this paper, microfluidic chip was developed for studying production ROS lung cancer cells under different chemical physical stimuli. This has two unique features: (1) five relative concentrations 0, 1/8, 1/2, 7/8, 1 achieved culture regions; (2) shear stress gradient is produced inside each areas. Lung were seeded biocompatible investigating...

Thallium is considered as an emergent contaminant owing to its potential use in the superconductor alloys. The monovalent thallium, Tl(I), highly toxic animals it can affect numerous metabolic processes. Here we observed that Tl(I) decreased protein synthesis and phosphorylated eukaryotic initiation factor 2α. Although has been shown interact with sulfhydryl groups of proteins cause accumulation reactive oxygen species, did not activate endoplasmic reticulum stress. Notably, level 60S...

Puf6 and Loc1 have two important functional roles in the cells, asymmetric mRNA distribution ribosome biogenesis. are localized predominantly nucleolus. They bind ASH1 mRNA, repress its translation, facilitate transport to daughter cells. Asymmetric is for cell differentiation. Besides their localization, been shown be involved 60S In puf6Δ or loc1Δ pre-rRNA processing export impaired subunits underaccumulated. The studies of focused on pathway, but biogenesis still not clear. this study, we...

Directed cell migration can be driven by a range of external stimuli, such as spatial gradients of: chemical signals (chemotaxis); adhesion sites (haptotaxis); or temperature (thermotaxis). Continuum models typically include diffusion term to capture the undirected component motility and an advection directed motility. However, there is no consensus in literature about form that takes. Some theoretical studies suggest ought receptor saturation effects. others adopt much simpler constant...

Biocides are frequently used to control sulfate-reducing bacteria (SRB) in biofouling. The increasing restrictions of environmental regulations and growing safety concerns on the use biocides result efforts minimize amount biocide develop environmentally friendly biocides. In this study, antimicrobial activity corrosion inhibition effect a low-toxic alternative biocide, benzyldimethyldodecylammonium chloride (BDMDAC), 304 stainless steel substrate immersed Desulfovibrio desulfuricans (D....

Collective cell migration plays important roles in many physiological processes such as embryonic development, tissue repair, and angiogenesis. A "wound" occurs when epithelial cells are lost and/or damaged due to some external factors, collective takes place the following wound-healing process. To study this cellular behavior, various kinds of assays developed. In these assays, a "wound," or "cell-free region," is created monolayer mechanically, chemically, optically, electrically. These...

Article28 January 2021Open Access Source DataTransparent process Poly(U)-specific endoribonuclease ENDOU promotes translation of human CHOP mRNA by releasing uORF element-mediated inhibition Hung-Chieh Lee Institute Biomedical Sciences, Mackay Medical College, New Taipei City, TaiwanThese authors contributed equally to this work Search for more papers author Chuan-Yang Fu Cheng-Yung Lin Taiwan Jia-Rung Hu Molecular and Cellular Biology, National University, Taipei, Ting-Ying Huang Kai-Yin Lo...

Large cargoes require multiple receptors for efficient transport through the nuclear pore complex. The 60S ribosomal subunit is one of bulkiest cargoes, and in yeast three different receptors, Crm1, Mex67/Mtr2, Arx1, collaborate its export. However, only recruited by adapter Nmd3, appears to be conserved export higher eukaryotes. We asked if large requires specific receptors. made protein fusions between mutant Nmd3 various Surprisingly, Mex67, tRNA exportin Los1, Mtr2, Cse1, or Msn5 lacking...