A5089181540- RNA and protein synthesis mechanisms
- Sirtuins and Resveratrol in Medicine
- Calcium signaling and nucleotide metabolism
- DNA and Nucleic Acid Chemistry
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- DNA Repair Mechanisms
- RNA modifications and cancer
- Adenosine and Purinergic Signaling
- RNA Interference and Gene Delivery
- PARP inhibition in cancer therapy
- Adipose Tissue and Metabolism
- Autophagy in Disease and Therapy
- Epigenetics and DNA Methylation
- Antimicrobial Peptides and Activities
- Chemical Synthesis and Analysis
- Biochemical and Molecular Research
- Click Chemistry and Applications
- Biotin and Related Studies
- Prion Diseases and Protein Misfolding
- Cancer therapeutics and mechanisms
- Nicotinic Acetylcholine Receptors Study
- Electron Spin Resonance Studies
- ATP Synthase and ATPases Research
Peter the Great St. Petersburg Polytechnic University
2015-2024
Institute of Cytology
2022-2023
Kurchatov Institute
2012-2021
Petersburg Nuclear Physics Institute
2012-2021
Purdue University West Lafayette
2016
Nicotinamide riboside (NR), a new form of vitamin B3, is an effective precursor nicotinamide adenine dinucleotide (NAD+) in human and animal cells. The introduction NR into the body effectively increases level intracellular NAD+ thereby restores physiological functions that are weakened or lost experimental models aging various pathologies. Despite active use applied biomedicine, mechanism its transport mammalian cells currently not understood. In this study, we used overexpression proteins...
The RecX protein, a very active natural RecA protein inhibitor, can completely disassemble filaments at nanomolar concentrations that are two to three orders of magnitude lower than protein. Based on the structure complex with presynaptic filament, we designed short first in class α-helical peptide both inhibits activities vitro and blocks bacterial SOS-response vivo. was using SEQOPT, novel method for global sequence optimization α-helices. SEQOPT produces artificial sequences containing...
α-Helices are the most frequently occurring elements of secondary structure in water-soluble globular proteins. Their increased conformational stability is among main reasons for high thermal proteins thermophilic bacteria. In addition, α-helices often involved protein interactions with other proteins, nucleic acids, and lipids cell membranes. That why highly stable α-helical peptides used as active specific inhibitors protein-protein have recently found more applications medicine. Several...
Nicotinamide adenine dinucleotide (NAD) is an essential redox carrier, whereas its degradation a key element of important signaling pathways. Human cells replenish their NAD contents through biosynthesis from extracellular precursors. These precursors encompass bases nicotinamide (Nam) and nicotinic acid corresponding nucleosides riboside (NR) (NAR), now collectively referred to as vitamin B3. In addition, NAD+ mononucleotide (NMN), potentially deamidated counterparts, (NAAD) (NAMN), may...
Nicotinamide adenine dinucleotide (NAD) and its phosphorylated form, NADP, are the major coenzymes of redox reactions in central metabolic pathways. is also used to generate second messengers, such as cyclic ADP-ribose, serves substrate for protein modifications including ADP-ribosylation deacetylation by sirtuins. The regulation these signaling processes depends on NAD availability. Generally, human cells accomplish their supply through biosynthesis using different forms vitamin B3: (Nam)...
Abstract A large variety of short biologically active peptides possesses antioxidant, antibacterial, antitumour, anti-ageing and anti-inflammatory activity, involved in the regulation neuro-immuno-endocrine system functions, cell apoptosis, proliferation differentiation. Therefore, mechanisms their biological activity are attracting increasing attention not only modern molecular biology, biochemistry biophysics, but also pharmacology medicine. In this work, we systematically analysed ability...
Nicotinamide riboside (NR) is an effective precursor of nicotinamide adenine dinucleotide (NAD) in human and animal cells. NR supplementation can increase the level NAD various tissues thereby improve physiological functions that are weakened or lost experimental models aging pathologies. However, there also reports questioning efficacy supplementation. Indeed, mechanisms its utilization by cells not fully understood. Herein, we investigated role purine nucleoside phosphorylase (PNP)...
Streptomycetes produce a huge variety of bioactive metabolites, including antibiotics, enzyme inhibitors, pesticides and herbicides, which offer promise for applications in agriculture as plant protection growth-promoting products. The aim this report was to characterize the biological activities strain Streptomyces sp. P-56, previously isolated from soil an insecticidal bacterium. metabolic complex obtained liquid culture P-56 dried ethanol extract (DEE) possessed activity against vetch...
RecA protein mediates homologous recombination repair in bacteria through assembly of long helical filaments on ssDNA an ATP-dependent manner. RecX, important negative regulator RecA, is known to inhibit activity by stimulating the disassembly nucleoprotein filaments. Here we use a single-molecule approach address regulation (Escherichia coli) RecA-ssDNA RecX (E. within framework distinct conformational states filament. Our findings revealed that effectively binds inactive conformation and...
Maintenance of the cellular redox balance has vital importance for correcting organism functioning. Methionine sulfoxide reductases (Msrs) are among key members antioxidant defence system. To work properly, methionine need to be reduced by their biological partner, thioredoxin (Trx). This process, according available kinetic data, represents slowest step in Msrs catalytic cycle. In present paper, we investigated structural aspects intermolecular complex formation between mammalian MsrB1 and...
The complete decipherment of the functions and interactions elements riboflavin biosynthesis operon (rib operon) Bacillus subtilis are necessary for development superproducers this important vitamin. function its terminal ribT gene has not been established to date. In work, a search homologs hypothetical amino acid sequence product through databases, as well an analysis homolgs, was performed; distribution secondary structure theoretically predicted; tertiary RibT protein proposed....
The RecA protein plays a key role in bacterial homologous recombination (HR) and acts through assembly of long helical filaments around single-stranded DNA the presence ATP. Large-scale conformational changes induced by ATP hydrolysis result transitions between stretched compressed forms filament. Here, using single-molecule approach, we show that nucleoprotein can exist two distinct interconvertible states depending on ADP monomer-monomer interface. Binding promotes cooperative directly...
Deinococcus radiodurans (Dr) has one of the most robust DNA repair systems, which is capable withstanding extreme doses ionizing radiation and other sources damage. DrRecA, a central enzyme recombinational repair, essential for radioresistance. In presence ATP, DrRecA forms nucleoprotein filaments on DNA, similar to bacterial RecA eukaryotic strand exchange proteins. However, catalyzes in unique reverse pathway. Here, we study dynamics formed individual molecules duplex single-stranded...
Abstract RecA protein mediates homologous recombination repair in bacteria through assembly of long helical filaments on single-stranded DNA (ssDNA) an ATP dependent manner. RecX, important negative regulator RecA, is known to inhibit activity by stimulating the disassembly nucleoprotein filaments. Here we use a single-molecule approach address regulation ( E. coli ) RecA-ssDNA RecX within framework distinct conformational states filament. Our findings revealed that effectively binds...