A5085210202- Plant Molecular Biology Research
- Plant Micronutrient Interactions and Effects
- Photosynthetic Processes and Mechanisms
- Metalloenzymes and iron-sulfur proteins
- Plant Gene Expression Analysis
- Plant Reproductive Biology
- Seed Germination and Physiology
- Ubiquitin and proteasome pathways
- Plant Stress Responses and Tolerance
- Iron oxide chemistry and applications
- Autophagy in Disease and Therapy
- Plant nutrient uptake and metabolism
- Endoplasmic Reticulum Stress and Disease
- Plant tissue culture and regeneration
- Electrocatalysts for Energy Conversion
- Enzyme Structure and Function
- Receptor Mechanisms and Signaling
- Nanocluster Synthesis and Applications
- Fermentation and Sensory Analysis
- Plant responses to water stress
- Cassava research and cyanide
- Antimicrobial Peptides and Activities
- Phosphodiesterase function and regulation
- Plant Physiology and Cultivation Studies
- Magnetic and Electromagnetic Effects
Centre National de la Recherche Scientifique
2015-2023
Institut des Sciences des Plantes de Montpellier
2015-2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2016-2023
Institut Agro Montpellier
2019-2023
Université de Montpellier
2019-2023
L'Institut Agro
2021-2022
Institut Jean-Pierre Bourgin
2009-2016
AgroParisTech
2009-2015
Université Paris-Saclay
2015
Institut National de la Recherche Agronomique
2000-2014
The G protein-coupled receptor (GPCR) superfamily represents the most important class of pharmaceutical targets. Therefore, characterization cascades and their ligands is a prerequisite to discovering novel drugs. Quantification agonist-induced second messengers downstream-coupled kinase activities central GPCRs or other pathways that converge on GPCR-mediated signaling. Furthermore, there need for simple, cell-based assays would report direct indirect actions effectors More generally,...
Abstract LEAFY COTYLEDON2 (LEC2) is a master regulator of seed development in Arabidopsis thaliana. In vegetative organs, LEC2 expression negatively regulated by Polycomb Repressive Complex2 (PRC2) that catalyzes histone H3 Lys 27 trimethylation (H3K27me3) and plays crucial role developmental phase transitions. To characterize the cis-regulatory elements involved transcriptional regulation LEC2, molecular dissections functional analyses promoter region were performed vitro, both yeast...
Summary TT 8 / b HLH 042 is a key regulator of anthocyanins and proanthocyanidins ( PA s) biosynthesis in A rabidopsis thaliana . transcriptional activity has been studied extensively, relies on its ability to form, with several R 2 3‐ MYB TTG 1 WD ‐ epeat protein), different bHLH WDR MBW ) protein complexes. By contrast, little known how expression itself regulated. Transcriptional regulation was using molecular, genetic biochemical approaches. Functional dissection the promoter revealed...
The control of growth and development all living organisms is a complex dynamic process that requires the harmonious expression numerous genes. Gene mainly controlled by activity sequence-specific DNA binding proteins called transcription factors (TFs). Amongst various classes eukaryotic TFs, MYB superfamily one largest most diverse, it has considerably expanded in plant kingdom. R2R3-MYBs have been extensively studied over last 15 years. However, DNA-binding specificity characterized for...
Abstract In the exalbuminous species Arabidopsis thaliana, seed maturation is accompanied by deposition of oil and storage proteins reduction endosperm to one cell layer. Here, we consider reserve partitioning between embryo compartments. The pattern deposition, final amount, composition these reserves differ two compartments, with representing principal tissue in mature seeds. Complex regulatory mechanisms are known prevent activation maturation-related programs during morphogenesis and,...
In Arabidopsis (Arabidopsis thaliana), transcriptional control of seed maturation involves three related regulators with a B3 domain, namely LEAFY COTYLEDON2 (LEC2), ABSCISIC ACID INSENSITIVE3 (ABI3), and FUSCA3 (ABI3/FUS3/LEC2 [AFLs]). Although genetic analyses have demonstrated partially overlapping functions these regulators, the underlying molecular mechanisms remained elusive. The results presented here confirmed that proteins bind RY DNA elements (with 5′-CATG-3′ core sequence) but...
The hydroxysteroid dehydrogenase HSD1, identified in the proteome of oil bodies from mature Arabidopsis seeds, is encoded by At5g50600 and At5g50700, two gene copies anchored on a duplicated region chromosome 5. Using real-time quantitative reverse transcriptase–polymerase chain reaction (RT–PCR) approach, accumulation HSD1 mRNA was shown to be specifically highly induced oil-accumulating tissues maturing seeds. disappeared during germination. activity promoter localization transcripts situ...
Abstract Iron–sulfur (Fe-S) proteins have critical functions in plastids, notably participating photosynthetic electron transfer, sulfur and nitrogen assimilation, chlorophyll metabolism, vitamin or amino acid biosynthesis. Their maturation relies on the so-called SUF (sulfur mobilization) assembly machinery. Fe-S clusters are synthesized de novo a scaffold protein complex then delivered to client via several transfer proteins. However, pathways of most their specificities for mostly...
Summary Screening of 10 000 Arabidopsis transgenic lines carrying a gene‐trap (GUS) construct has been undertaken to identify markers seed germination. One these showed GUS activity restricted the endosperm, at micropylar end germinating seed. The genomic DNA flanking T‐DNA insert was cloned by walking PCR and insertion shown be located 70 bp upstream 2285 open reading frame ( AtEPR1 ) sharing strong similarities with extensins. consists 40 proline‐rich repeats is expressed in both wild‐type...
Numerous proteins require a metallic co-factor for their function. In plastids, the maturation of iron-sulfur (Fe-S) necessitates complex assembly machinery. this study, we focused on Arabidopsis thaliana NFU1, NFU2, and NFU3, which participate in final steps process. According to strong photosynthetic defects observed high chlorophyll fluorescence 101 (hcf101), nfu2, nfu3 plants, determined that NFU2 but not act immediately upstream HCF101 [Fe4S4]-containing photosystem I subunits. An...
Abstract Ectopic expression of defensins in plants correlates with their increased capacity to withstand abiotic and biotic stresses. This applies Arabidopsis thaliana, where some the seven members PLANT DEFENSIN 1 family (AtPDF1) are recognised improve plant responses necrotrophic pathogens increase seedling tolerance excess zinc (Zn). However, few studies have explored effects decreased endogenous defensin on these stress responses. Here, we carried out an extensive physiological...
Ethylene regulates fruit ripening and several plant functions (germination, growth, plant-microbe interactions). Protein quantification of ethylene receptors (ETRs) is essential to study their functions, but impaired by low resolution tools such as antibodies that are mostly nonspecific, or the lack sensitivity shotgun proteomic approaches. We developed a targeted method, quantify low-abundance proteins ETRs, coupled this mRNAs analyses, in two tomato lines: Wild Type (WT) Never-Ripe (NR)...
Osmotic stress can be detrimental to plants, whose survival relies heavily on proteomic plasticity. Protein ubiquitination is a central post-translational modification in osmotic-mediated stress. In this study, we used the K-Ɛ-GG antibody enrichment method integrated with high-resolution mass spectrometry compile list of 719 ubiquitinated lysine (K-Ub) residues from 450 Arabidopsis root membrane proteins (58% which are transmembrane proteins), thereby adding database substrates plants....
The release of carbon dioxide (CO2) into the atmosphere has accelerated during last two decades. Elevated atmospheric CO2 concentration (eCO2) is known as an agent that improves plant photosynthesis. However, eCO2 was also correlated with alterations in macronutrient and micronutrient compositions various dietary crops. In order to explore effect on nutritional health properties tomatoes, three parental lines Magic population, which includes a large part genetic diversity present fruit...
Iron-sulfur (Fe-S) proteins play critical functions in plants. Most Fe-S are synthetized the cytosol as apo-proteins and subsequent cluster incorporation relies on specific protein assembly machineries. They notably formed by a scaffold complex, which serves for de novo synthesis, transfer that insure delivery to apo-targets. However, scarce information is available about maturation pathways of most plastidial their specificities towards associated SUF machinery. To gain more insights into...
Abstract Osmotic stress can be detrimental to plants, whose survival relies heavily on proteomic plasticity. Protein ubiquitination is a central post-translational modification in osmotic mediated stress. Plants use the ubiquitin (Ub) proteasome system modulate protein content, and role for Ub mediating endocytosis trafficking plant plasma membrane proteins has recently emerged. In this study, we used K-ε-GG antibody enrichment method integrated with high-resolution mass spectrometry compile...