Increasing greenhouse gas emissions have resulted in greater motivation to find novel carbon dioxide (CO2 ) reduction technologies, where the of CO2 valuable chemical commodities is desirable. Molybdenum-dependent formate dehydrogenase (Mo-FDH) from Escherichia coli a metalloenzyme that able interconvert and . We describe low-potential redox polymer, synthesized by facile method, contains cobaltocene (grafted poly(allylamine), Cc-PAA) simultaneously mediate electrons Mo-FDH immobilize at...

We demonstrate a novel hydrogel material to facilitate direct bioelectrochemistry of wide range redox proteins and enable ATP-independent electroenzymatic reduction N₂ by nitrogenase.

Nitrogenase, the only enzyme known to be able reduce dinitrogen (N2) ammonia (NH3), is irreversibly damaged upon exposure molecular oxygen (O2). Several microbes, however, are grow aerobically and diazotrophically (fixing N2 grow) while containing functional nitrogenase. The obligate aerobic diazotroph, Azotobacter vinelandii, employs a multitude of protective mechanisms preserve nitrogenase activity, including "conformational switch" protein (FeSII, or "Shethna") that reversibly locks into...

Abstract Increasing greenhouse gas emissions have resulted in greater motivation to find novel carbon dioxide (CO 2 ) reduction technologies, where the of CO valuable chemical commodities is desirable. Molybdenum‐dependent formate dehydrogenase (Mo‐FDH) from Escherichia coli a metalloenzyme that able interconvert and . We describe low‐potential redox polymer, synthesized by facile method, contains cobaltocene (grafted poly(allylamine), Cc‐PAA) simultaneously mediate electrons Mo‐FDH...

Massive efforts are invested in developing innovative CO2 -sequestration strategies to counter climate change and transform into higher-value products. -capture by reduction is a chemical challenge, attention turned toward biological systems that selectively efficiently catalyse this reaction under mild conditions aqueous solvents. While few reports have evaluated the effectiveness of isolated bacterial formate dehydrogenases as catalysts for reversible electrochemical , it imperative...

Ferredoxins are small iron-sulfur proteins that engage in electron transfer (ET) with oxidoreductases across all domains of life. In bacteria, ferredoxins contain two [4Fe-4S] clusters differ respect to their electric midpoint potential: while “Alvin”-type show individual potentials between -500 and -650 mV vs. SHE, clostridial perform ET at indistinguishable approximately -400 SHE. this work, the complex ferredoxin CpFd [FeFe]-hydrogenases from Clostridium pasteurianum (CpI) green algae...

Formate/formic acid has been an intriguing fuel for cells and biofuel over the last two decades. The common challenge with formate/formic use of NAD-dependent formate dehydrogenase, which requires a diffusive cofactor problematic regeneration systems. In this paper, we explore bioelectro-oxidation using Mo-containing dehydrogenase from E. coli (Mo-FDH) development new HCOO−/O2 enzymatic cell (EFC). Mo-FDH was coupled benzylpropylviologen-based linear polyethylenimine (BPV-LPEI) redox polymer...

A major electron carrier involved in energy and carbon metabolism the acetogenic model organism Thermoanaerobacter kivui is ferredoxin, an iron-sulfur-containing, electron-transferring protein. Here, we show that genome of T. encodes four putative ferredoxin-like proteins (TKV_c09620, TKV_c16450, TKV_c10420 TKV_c19530). All genes were cloned, a His-tag encoding sequence was added produced from plasmid kivui. The purified had absorption peak at 430 nm typical for ferredoxins. determined...

An endo-β-1,4-glucanase (EG), produced under submerged fermentation by local isolate Trichoderma atroviride, was purified using ammonium sulfate precipitation, column and ion exchange chromatography with 55.16 fold a specific activity of 30.9 EU mg -1 .The studies PAGE, SDS-PAGE zymogram test have been carried out.The EG had optimum at pH 5.0 50 °C respectively.Using CMC as substrate, the enzyme showed maximum (V max ) 6.7 (µmol glucose min mL its corresponding K m (Michaelis-Menten...

Abstract Protease from Bacillus subtilis M‐11 was produced and purified using ammonium sulfate precipitation, gel filtration chromatography with 15.8‐fold. The enzyme showed a single band on sodium dodecyl sulfate‐polyacrylamide electrophoresis, it determined that its molecular weight 32 kDa. immobilized polysulfone membrane containing Silica gel‐3 aminopropyltriethoxysilane (Si‐APTS) by physical adsorption. optimal immobilization conditions (pH, temperatures, incubation time) were...

The novel coronavirus disease because of infection with the SARS-CoV-2 virus, COVID-19, was first appeared in Wuhan, China, December 2019. It has spread rapidly all around World and been accepted as a pandemic. Specific therapies for COVID-19 treatment is not available now. Thus, there huge effort to develop discover new therapeutic agents vaccines by scientists. design development through medicinal chemistry slow needed hard labor process. it urgent achieve discovery more effective agents....

Abstract Massive efforts are invested in developing innovative CO 2 ‐sequestration strategies to counter climate change and transform into higher‐value products. ‐capture by reduction is a chemical challenge, attention turned toward biological systems that selectively efficiently catalyse this reaction under mild conditions aqueous solvents. While few reports have evaluated the effectiveness of isolated bacterial formate dehydrogenases as catalysts for reversible electrochemical , it...

In this study, TiO2 nanoparticles were prepared and -COOH functionalized with 3-(3,4-dihydroxyphenyl) propionic acid. The characterization of was performed by FTIR, TEM, EDS XRD. HRP immobilized on those EDC/NHS coupling reaction. immobilization conditions including A: enzyme concentration (0.5-1.5 mg/mL), B: pH (4.0-8.0), C: temperature (4-50°C), D: time (1-20 h) optimized response surface methodology Box-Behnken design. identified as 0.5 mg/mL HRP, at 5.5, 40 °C for 8 h activity 1.5 4 18°C...

Pectinase from Aspergillus tamari strain Redpep4 was immobilized on Zr-treated pumice by adsorption. The support characterized FTIR, SEM, XPS, and EDS analysis. effects of various factors immobilization such as the initial amount enzyme (25 mg, 50 150 300 mg), time (1 hr, 3 6 18 hr), temperature (4°C, 25°C, 40°C, 50°C) were examined. maximum adsorbed pectinase determined 229 mg/g support. optimal pH for activity shifted 6.0 to 7.0 after immobilization; however, optimum remained unchanged at...

Cellulose is major source of plant biomass and β-1,4-glucosidic bonds in its structure are hydrolyzed by cellulases.These enzymes can be produced microorganisms including fungi, bacteria actinomycetes used today for the industrial applications pulp paper, food textile industries conversion materials into industrially useful products such as sugars bio-ethanol.The cost production low yields these problems applications.For this reason, there a requirement that new microbial enzyme sources...

In this study, 6-phosphogluconate dehydrogenase was covalently immobilized onto the N-2-aminoethyl-3-aminopropyltriethoxysilane (APTES) modified core-shell Fe3O4@SiO2 magnetic nanoparticles (ASMNPs) using glutaraldehyde (GA). Immobilization of 6PGDH on ASMNPs confirmed fourier transform-infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) analysis. The NADP+ conversion ratio, reusability, thermal, storage stability were determined compared with those free enzyme. maximum...

Antihistamines and glucocorticoids are commonly used to treat allergy symptoms the inflammatory conditions. In present study, in-vitro binding interactions a glucocortikoid, dexamethasone/an antihistamine, pheniramine with TSN (TSN) secreted from pancreas small intestine for protein digestion were investigated by fluorescence emission spectroscopy (FES), UV-Vis spectroscopy, synchronous (SFS), CD FT-IR molecular modeling methods. Also, effect of these drugs on catalytic activity trypsin was...