A5082775766- Protein Structure and Dynamics
- Enzyme Structure and Function
- Hepatitis C virus research
- Biochemical and Molecular Research
- Monoclonal and Polyclonal Antibodies Research
- Air Quality and Health Impacts
- RNA and protein synthesis mechanisms
- Liver Disease Diagnosis and Treatment
- Air Quality Monitoring and Forecasting
- HIV/AIDS drug development and treatment
- Click Chemistry and Applications
- Genetics, Bioinformatics, and Biomedical Research
- Glutathione Transferases and Polymorphisms
- Enzyme function and inhibition
- Urban Green Space and Health
- Artificial Intelligence in Healthcare
- Genomics, phytochemicals, and oxidative stress
- Migration, Aging, and Tourism Studies
- Genomics and Chromatin Dynamics
- Inflammatory Bowel Disease
- Glycosylation and Glycoproteins Research
- Cancer Research and Treatments
- Cancer-related gene regulation
- IL-33, ST2, and ILC Pathways
- Liver Disease and Transplantation
Jordan University of Science and Technology
2025
Weill Cornell Medical College in Qatar
2019-2024
Homerton University Hospital
2023
University of New Mexico
2022
Security Forces Hospital
2022
Cairo University
2005-2021
Southern Illinois University Edwardsville
2013-2016
Texas Tech University
2009-2014
Texas Tech University Health Sciences Center
2009-2014
Mahidol University
2012
The bacterial melibiose permease (MelB) belongs to the glycoside–pentoside–hexuronide:cation symporter family, a part of major facilitator superfamily (MFS). Structural information regarding family transporters and other Na+-coupled permeases within MFS has been lacking, although wealth biochemical biophysical data are available. Here we present three-dimensional crystal structures Salmonella typhimurium MelBSt in two conformations, representing an outward partially occluded inactive state...
Precision medicine—of which precision prescribing is a core component—is becoming new frontier in today’s healthcare. Both artificial intelligence (AI) and machine learning (ML) have the potential to enhance our understanding of data therefore ability accurately diagnose treat patients. By leveraging these technologies processes, we can uncover associations between person’s genomic makeup their health, identify biomarkers associated with diseases, fine-tune patient selection for clinical...
Abstract Arginine kinase (AK) is a member of the guanidino family that plays an important role in buffering ATP concentration cells with high and fluctuating energy demands. The AK specifically catalyzes reversible phosphoryl transfer between arginine. We have determined crystal structure from horseshoe crab ( Limulus polyphemus ) its open (substrate‐free) form. final model has been refined at 2.35 Å R 22.3% (R free = 23.7%). form compared to previously transition state analog complex closed...
Abstract Background Weight reduction can effectively improve non-alcoholic fatty liver disease (NAFLD), which is a constant companion of severe obesity. This study aimed to determine the effect one anastomosis gastric bypass (OAGB) on pathological changes in severely obese cases with NAFLD. Methods The present prospective research comprised 67 subjects morbid obesity scheduled for OAGB during period from February 2015 August 2018. Clinical, biological, and histologic data were evaluated pre-...
The three-dimensional crystal structure of an arginine kinase transition-state analogue complex has been refined at 1.2 Å resolution, with overall R factor 12.3%. current model provides a unique opportunity to analyze the bimolecular (phosphagen kinase) enzyme in its transition state. This atomic resolution confirms in-line transfer phosphoryl group and catalytic importance precise alignment substrates. is consistent concerted proton that proposed for unrelated kinase. Refinement anisotropic...
Arginine kinase is a member of the phosphagen family that includes creatine and likely shares common reaction mechanism in catalyzing buffering cellular ATP energy levels. Abstraction proton from substrate guanidinium by catalytic base has long been thought to be an early mechanistic step. The structure arginine as transition state analog complex (Zhou, G., Somasundaram, T., Blanc, E., Parthasarathy, Ellington, W. R., Chapman, M. S. (1998) Proc. Natl. Acad. Sci. U. A. 95, 8449–8454) showed...
The melibiose permease of Escherichia coli (MelB) catalyzes the coupled stoichiometric symport a galactoside with cation (either Na(+), Li(+), or H(+)), using free energy from downhill translocation one cosubstrate to catalyze accumulation other. Here, we present 3D structure model MelB threaded through crystal lactose E. (LacY), manually adjusted, and energetically minimized. contains 442 consecutive residues ( approximately 94% polypeptide), including all 12 transmembrane helices...
The protease domain of the Hepatitis C Virus (HCV) nonstructural protein 3 (NS3) has been targeted for inhibition by several direct-acting antiviral drugs. This approach had marked success to treat infections caused HCV genotype 1 predominant in USA, Europe, and Japan. However, genotypes 4, dominant developing countries, are resistant a number these drugs little progress made towards understanding structural basis their drug resistivity. We have previously developed 4D computational...
The misfolding and aggregation of human islet amyloid polypeptide (hIAPP), also known as amylin, have been implicated in the pathogenesis type 2 diabetes (T2D). Heat shock proteins, specifically, heat cognate 70 (Hsc70), are molecular chaperones that protect against hIAPP inhibits its aggregation. Nevertheless, there is an incomplete understanding mechanistic interactions between Hsc70 domains hIAPP, thus limiting their potential therapeutic role diabetes. This study investigates inhibitory...
G protein–coupled receptors are ubiquitous in neurons, as well other cell types. Activation of by hormones or neurotransmitters splits the protein heterotrimer into Gα and Gβγ subunits. It is now clear that directly inhibits Ca 2 + channels, putting them a reluctant state. The effects depend on specific β γ subunits present, subunit isoform N-type channel. We describe minimal mathematical model for action dynamics synaptic transmission. calibrated data obtained transfecting channel tsA-201...
We have designed a molecular switch in T4 lysozyme construct that controls large-scale translation of duplicated helix. As shown by crystal structures the with on and off, conformational change is triggered binding ligand (guanidinium ion) to site wild-type protein was occupied guanidino head group an Arg. In design template, helix flanked two loop regions different stabilities. "on" state, N-terminal weakly structured, whereas C-terminal has well defined conformation stabilized means...
BL-17A is a new structural biology beamline at the Photon Factory, Japan. The high-brilliance beam, derived from short-gap undulator (SGU#17), allows for unique protein crystallographic experiments such as data collection microcrystals and determination using softer X-rays. However, microcrystal require robust beam stability during minor fluctuations could not be ignored. Initially, significant instability was observed BL-17A. causes of were investigated its various sources identified....
Miranda is a multidomain adaptor protein involved in neuroblast asymmetric division Drosophila melanogaster. The central domain of necessary for cargo binding the neural transcription factor Prospero, Prospero-mRNA carrier Staufen, and tumor suppressor Brat. Here, we report first solution structure "cargo-binding" (residues 460-660) using small-angle X-ray scattering. Ab initio modeling scattering data yields an elongated "rod-like" molecule with maximum linear dimension (D(max))...
Egypt has the highest prevalence of hepatitis C virus (HCV) infection worldwide with a frequency 15%. More than 90% these infections are due to genotype 4, and subtype 4a (HCV-4a) predominates. Moreover, increased mobility people, HCV-4a recently spread several European countries. The protease domain HCV nonstructural protein 3 (NS3) been targeted for inhibition by drugs. This approach had marked success in inhibiting 1 (HCV-1), predominant USA, Europe, Japan. However, was found resist...
Perhaps the main goal of healthcare management is attainment effective, efficient, equitable, timely, safe, and patient-centered care. At core this lies need for an integrated pathway data storage, analysis, utilization. The potential exists a centralized, cloud-based system that links physicians, hospitals, public health agencies, insurance pharmaceutical companies, most importantly, patients. Such could improve clinical quality support delivery consistent effective treatments. Undoubtedly,...
We are developing a 4D computational methodology, based on 3D structure modeling and molecular dynamics simulation, to analyze the active site of HCV NS3 proteases, in relation their catalytic activity. In our previous work, analyses interactions between triad residues (His57, Asp81, Ser139) yielded divergent, gradual genotype-dependent, conformational instability measures, which strongly correlate with known disparate activities among genotypes. Here, correlation geometrical measure is...