A5101876523- Hepatitis C virus research
- Hepatitis B Virus Studies
- Monoclonal and Polyclonal Antibodies Research
- Liver Disease Diagnosis and Treatment
- Virology and Viral Diseases
- Protein Degradation and Inhibitors
- Biochemical and Molecular Research
- HIV/AIDS drug development and treatment
- Ubiquitin and proteasome pathways
- Hepatocellular Carcinoma Treatment and Prognosis
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- SARS-CoV-2 and COVID-19 Research
- Hemoglobinopathies and Related Disorders
- Education and Islamic Studies
- Pediatric Hepatobiliary Diseases and Treatments
- Viral Infections and Immunology Research
- Microbial infections and disease research
- Cellular transport and secretion
- Gallbladder and Bile Duct Disorders
- Endoplasmic Reticulum Stress and Disease
- Jewish and Middle Eastern Studies
- Islamic Studies and History
- Pancreatitis Pathology and Treatment
GIFT University
2025
Shenzhen Bay Laboratory
2024-2025
Sun Yat-sen University
2018-2023
Southern Medical University
2019-2022
Nanfang Hospital
2019-2022
Hainan Medical University
2021
Institute of Human Virology
2021
Hainan General Hospital
2021
Pakistan Health Research Council
2018
Jinnah Postgraduate Medical Center
2016-2018
Abstract Lysosome‐targeting chimeras (LYTACs) represent a promising approach for the targeted degradation of membrane proteins. Currently, two primary methods LYTAC development involve chemically modified antibodies and wild‐type insulin‐like growth factor 2 (IGF2) fusion proteins (iLYTACs). However, LYTACs necessitate intricate chemical modification processes, while IGF2 in iLYTAC technology binds to IGF1R, potentially triggering carcinogenesis. To tackle this challenge, we introduce...
Background: Prolonged neonatal cholestasis is a critical condition requiring early differentiation between biliary atresia and hepatitis to ensure timely intervention. Biliary necessitates surgical correction, whereas managed medically. Accurate diagnosis essential prevent delays in treatment avoid unnecessary procedures. Although hepatobiliary iminodiacetic acid (HIDA) scanning widely used, its availability limited many settings. Liver biopsy with histopathological evaluation considered...
Abstract Background To find out the prevalence of active hepatitis C virus (HCV) infections among general public in Lahore city, since data concerning HCV this city is currently unavailable. Methods Blood samples were collected randomly from individuals visiting different clinical laboratories Lahore. Serum was separated and processed by nested PCR qualitative assay for detection RNA. The categorized into age groups on basis pre-test questionnaires order to record age-wise differences...
Introduction. Transfusion Transmitted Infections (TTIs) continue to be a major risk in transfusions many parts of the world. The transfusion-dependent β-thalassaemia patients are particularly at acquiring TTIs. current study was undertaken estimate prevalence TTIs patients. Material and Methods. A cross-sectional 1253 multitransfused thalassaemia conducted five different centres Islamabad, Rawalpindi, Karachi. subjects were screened for HIV, HCV, HBV. screening performed two centres:...
SUMMARY We studied a cross-sectional sample of the population Kech, small rural town in Pakistan to determine prevalence and risk factors for hepatitis C infection. The was 110 out 2000 persons (5·5%, 95% confidence interval 4·5–6·5). Higher rates were identified males. Independent age ⩾75 years, being healthcare worker, injecting drug use. There high many potential routes transmission bloodborne viruses most people reported at least one factor.
The protease domain of the Hepatitis C Virus (HCV) nonstructural protein 3 (NS3) has been targeted for inhibition by several direct-acting antiviral drugs. This approach had marked success to treat infections caused HCV genotype 1 predominant in USA, Europe, and Japan. However, genotypes 4, dominant developing countries, are resistant a number these drugs little progress made towards understanding structural basis their drug resistivity. We have previously developed 4D computational...
Abstract Almost one third of the world's Muslims live as minorities in non-Muslim states. This article examines position West with special reference to United Kingdom. First, an overview Western states is presented, concentrating on States, Canada, Australia and Europe. Second, demographic socio-economic Britain discussed, particularly highlighting various issues facing them. Third, religious discrimination feeling insecurity among British Muslims, well state policies towards are examined....
Context: Natural strain variation and rapid resistance development makes of broad spectrum hepatitis C virus (HCV) drugs very challenging evaluation inhibitor selectivity must account for differences in the catalytic properties enzyme variants.Objective: To understand how to study relationships between efficacy genotype or resistant mutants NS3 protease inhibitors.Materials methods: The from genotypes 1a, 1b 3a, their sensitivities four structurally mechanistically different inhibitors have...
Hepatitis C virus (HCV) infection increased the risk of hepatocellular carcinoma. Identification host factors required for HCV will help to unveil pathogenesis. Adaptive mutations that enable replication infectious clones could provide hints mutation-carrying viral protein may specifically interact with some cellular essential life cycle. Previously, we identified D559G mutation in NS5B (RNA dependent RNA polymerase) important different genotype clones. Here, searched potentially interacted...
Abstract Background Hepatitis C virus (HCV) is a common and leading cause for liver cirrhosis hepatocellular carcinoma. Current therapies to treat HCV infection are shown be partially effective poorly tolerated. Therefore, ample efforts underway rationally design targeting the non-structural proteins. Most of work carried out in this direction has been focusing mainly on genotype 1. Two direct-acting antiviral agents (DAAs) Telaprevir Boceprevir being used against 1a combination therapy with...
To aid the design of next generation hepatitis C virus (HCV) drugs, kinetics interactions between NS3 protease inhibitors and enzyme from genotypes 1a, 1b, 3a have been characterized. The linear mechanism-based VX-950 (telaprevir) SCH 503034 (boceprevir) benefited covalent adduct formation. However, apparent affinities were rather weak (VX-950, K(D)* 340, 8.5, 1000 nM for 1b 3a, respectively; 503034, 90 3.9 respectively). non-mechanism-based macrocyclic BILN-2016 (ciluprevir) ITMN-191...
Objective: To assess the Histological alteration of liver in asymptomatic HCV PCR positive family blood donors.Methods: This is a prospective cross sectional study carried out at Blood Transfusion Services, Clinical & Molecular Laboratory and Pakistan Health Research Council, Jinnah Postgraduate Medical Centre, Karachi from January 2011 to December 2016. One hundred thirteen (113) Anti RNA donors with normal or slightly raised ALT during three consecutive bimonthly visits were included....
Hepatitis C virus (HCV) infection is a major cause of chronic hepatitis and liver cancer worldwide. Adaptive mutations play important roles in the development HCV replicon its infectious clones. We others have previously identified p7 mutation F772S co-presence NS4A full-length clones chimeric recombinants. However, underlying mechanism function remains incompletely understood. Here, we investigated functional role using an efficient JFH1-based reporter with Core-NS2 from genotype 2a strain...
To analyze the incidence and risk factors of benign liver space-occupying mass in patients with chronic hepatitis B (CHB) ultrasound features that differentiate these masses from small hepatocellular carcinoma.We retrospectively analyzed color Doppler clinical data 17 721 CHB treated Hepatology Unit Nanfang Hospital between January, 2016 December, 2017. The were compared those 21629 healthy control subjects undergoing routine physical examination Center Heath Management during same...
Abstract Lysosome-targeting chimeras (LYTACs) have emerged as a promising strategy for targeted degradation of membrane proteins, offering potential applications in drug development. Currently, two main methods developing LYTACs exist: chemically modified antibodies [1-2] and wild-type insulin-like growth factor 2 (IGF2) fusion proteins (iLYTACs) [3] . However, the IGF2 arm within iLYTACs carries risk activating IGF1R tyrosine kinase activity promoting tumor To address this concern, we...
Whether hypervariable region 1 (HVR1)-targeting antibodies elicited during natural hepatitis C virus (HCV) infection contribute to clearance and what is the mechanism underlying remain unclear. Here, we demonstrated that treatment of HCV-infected hepatoma Huh7.5 cells with IgGs purified from 2 28 (7.1%) chronic (CHC) patients efficiently controlled infection, for which genotype 1b HVR1 (1bHVR1)-binding antibody was critical. Moreover, found 1bHVR1 peptide superior 2aHVR1 in rabbit...
Abstract Background: Hepatitis C virus (HCV) is an important human pathogen causing chronic hepatitis C, end-stage liver diseases, and hepatocellular carcinoma. The development of infectious HCV cell culture systems primarily relied on the replication enhancement effect adaptive mutations. Although mode action may vary, those mutations could direct study virus-host interactions required for efficient infection. We previously identified a substitution D559G in NS5B (RNA dependent RNA...