A5038309864- Extracellular vesicles in disease
- Immunotherapy and Immune Responses
- Hepatitis C virus research
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Hepatitis B Virus Studies
- Ferroptosis and cancer prognosis
- Transplantation: Methods and Outcomes
- Nanoplatforms for cancer theranostics
- Cancer Immunotherapy and Biomarkers
- Advanced Nanomaterials in Catalysis
- Cytomegalovirus and herpesvirus research
- RNA regulation and disease
- Hippo pathway signaling and YAP/TAZ
- MXene and MAX Phase Materials
- Epigenetics and DNA Methylation
- Autophagy in Disease and Therapy
- Signaling Pathways in Disease
- Clostridium difficile and Clostridium perfringens research
- Liver Disease Diagnosis and Treatment
- Genomics and Chromatin Dynamics
- Wound Healing and Treatments
- Immune Cell Function and Interaction
- Diagnosis and Treatment of Venous Diseases
- interferon and immune responses
Sun Yat-sen University
2018-2024
The Seventh Affiliated Hospital of Sun Yat-sen University
2023-2024
Shenzhen University
2020
Ferroptosis, a form of iron-dependent cell death driven by cellular metabolism and lipid peroxidation, has been implicated as tumor-suppressor function for cancer therapy. Recent advance revealed that the sensitivity to ferroptosis is tightly linked numerous biological processes, including amino acid biosynthesis glutathione. Here, using high-throughput CRISPR/Cas9-based genetic screen in HepG2 hepatocellular carcinoma cells search metabolic proteins inhibiting ferroptosis, we identified...
Existing therapeutics for autoimmune diseases remain problematic due to low efficacy, severe side effects, and difficulties reach target tissues. Herein, we design multifunctional fusion nanovesicles that can lesions the treatment of skin diseases. The grapefruit-derived exosome-like (GEVs) with anti-inflammatory antioxidant effects are first encapsulated CX5461, an immunosuppressant anti-proliferative properties form GEV@CX5461. In order enhance therapeutic efficiency safety, GEV@CX5461...
Excessive and persistent inflammation after injury lead to chronic wounds, increased tissue damage or even aggressive carcinogenic transformation. Effective wound repair could be achieved by inhibiting overactive immune cells the injured site. In this study, we obtained high concentration of PD-L1 in exosomes from either genetically engineered overexpressing IFN-γ stimulated cells. We found that exosomal is specially bound PD-1 on T cell surface, suppressed activation. Interestingly,...
Ferroptosis, iron-dependent cell death, is an established mechanism for cancer suppression, particularly in hepatocellular carcinoma (HCC). Sorafenib (SOR), a frontline drug the treatment of HCC, induces ferroptosis by inhibiting Solute Carrier family 7 member 11 (SLC7A11), with inadequate notably contributing to SOR resistance tumor cells. To further verify biological targets associated analysis Cancer Genome Atlas (TCGA) database was performed find significant co-upregulation SLC7A11 and...
Heart transplantation offers life-saving treatment for patients with end-stage heart failure; however, ischemia-reperfusion injury (IRI) and subsequent immune responses remain significant challenges. Current therapies primarily target adaptive immunity, limited options available addressing IRI innate activation. Although plant-derived vesicle-like nanoparticles show promise in managing diseases, their application organ complications is unexplored. Here, this work develops a novel reactive...
T cell activation by immune allorecognition is a major contributing factor toward the triggering of organ rejection. Immunosuppressive drugs have to be taken after transplantation, but long-term use these increases risks infection and other serious disorders. Here, we showed dysregulation programmed death-ligand 1/programmed death 1 (PD-L1/PD-1) cytotoxic T-lymphocyte-associated protein 4/cluster differentiation 80 (CTLA-4/CD80) in spleen two transplantation models. Using bioengineering...
There is an urgent need for developing new immunosuppressive agents due to the toxicity of long-term use broad after organ transplantation. Comprehensive sample analysis revealed dysregulation FGL1/LAG-3 and PD-L1/PD-1 immune checkpoints in allogeneic heart transplantation mice clinical kidney transplant patients. In order enhance these two signal axes, a bioengineering strategy developed simultaneously display FGL1/PD-L1 (FP) on surface small extracellular vesicles (sEVs). Among various...
PD-L1 nanovesicles carrying rapamycin inhibit T cell activation to promote allograft acceptance.
PD-1 inhibitor Keytruda combined with chemotherapy for Triple-negative breast cancer (TNBC) has been approved FDA, successfully representing the combination therapy of immunotherapy and first time in 2020. However, PD-L1 Tecentriq albumin paclitaxel using similar strategy failed to achieve expected effect. Therefore, it is still necessary explore new effective chemotherapy-based strategies. We developed a cell membrane-derived programmed death-ligand 1(PD-1) nanovesicle encapsulate low-dose...
Although immunosuppressive drugs for targeting T cells are the standard of care in acute transplantation rejection, role innate immune should not be ignored. Here, single-cell RNA sequencing (scRNA-seq) and flow cytometry performed to reveal dynamic changes within rejection time find a significantly-increased presence Ly6G- Ly6C+ inflammatory macrophages decreased neutrophils among all types cells. Next, further explore potential targets regulating macrophages, scRNA-seq is used analyze...
Abstract Ferroptosis has been implicated as a tumor-suppressor function for cancer therapy. Recently the sensitivity to ferroptosis was tightly linked numerous biological processes, including metabolism of amino acid. Here, using high-throughput CRISPR/Cas9 based genetic screen in HepG2 cells search metabolic proteins inhibiting ferroptosis, we identified branched chain acid aminotransferase 2 (BCAT2) novel suppressor ferroptosis. Mechanistically, inducers (erastin, sorafenib and...
Mutations in the plant homeodomain-like finger protein 6 (PHF6) gene are strongly associated with acute myeloid (AML) and T-cell lymphoblastic leukemia (T-ALL). In this study, we demonstrated that PHF6 can bind to H3K9me3 H3K27me1 on nucleolar chromatin recruit histone methyltransferase SUV39H1 rDNA locus. The deletion of caused a decrease recruitment loci, resulting reduction level promotion transcription. knockdown either or significantly attenuated effects increase suppressed...
Hepatitis B virus (HBV) core protein (HBc) serves pivotal roles in the viral life cycle, particularly serving as basic unit for capsid assembly, and is closely associated with HBV genome replication progeny virion production. Previous studies have demonstrated that HBc has at least two functional interfaces; monomers form a homodimer via an intradimer interface, then 90 or 120 homodimers icosahedral dimer‑dimer interface. In present study, role of interface was investigated. A panel residues...
Currently, no vaccine to prevent hepatitis C virus (HCV) infection is available. A major challenge in developing an HCV the high diversity of sequences. The purpose immunization with viral glycoproteins induce a potent and long-lasting cellular humoral immune response. However, this strategy only achieves limited protection, antigen selection plays crucial role design. In study, we investigated responses induced by intraperitoneal injection keyhole limpet hemocyanin conjugated 4 highly...
Abstract There is an urgent need for developing new immunosuppressive agents due to the toxicity of long-term use broad post organ transplantation. Comprehensive sample analysis revealed dysregulation FGL1/LAG-3 and PD-L1/PD-1 immune checkpoints in allogeneic heart transplantation mice clinical kidney transplant patients. In order enhance these two signal axes, we developed a bioengineering strategy simultaneously display FGL1/PD-L1 (FP) on surface small extracellular vesicles (sEVs). Among...
Hepatitis C virus (HCV) infection is a major cause of chronic hepatitis and liver cancer worldwide. Adaptive mutations play important roles in the development HCV replicon its infectious clones. We others have previously identified p7 mutation F772S co-presence NS4A full-length clones chimeric recombinants. However, underlying mechanism function remains incompletely understood. Here, we investigated functional role using an efficient JFH1-based reporter with Core-NS2 from genotype 2a strain...
Whether hypervariable region 1 (HVR1)-targeting antibodies elicited during natural hepatitis C virus (HCV) infection contribute to clearance and what is the mechanism underlying remain unclear. Here, we demonstrated that treatment of HCV-infected hepatoma Huh7.5 cells with IgGs purified from 2 28 (7.1%) chronic (CHC) patients efficiently controlled infection, for which genotype 1b HVR1 (1bHVR1)-binding antibody was critical. Moreover, found 1bHVR1 peptide superior 2aHVR1 in rabbit...
Abstract Background Immunotherapy represented by the programmed death-1 (PD-1)/ligand 1 (PD-L1) monoclonal antibodies has led tumor treatment into a new era. However, low overall response rate and high incidence of drug resistance largely damage clinical benefits existing immune checkpoint therapies. Recent studies correlate to PD-1/PD-L1 blockade with PD-L1 expression levels in cells. Hence, identifying molecular targets pathways controlling protein stability cells is major priority....