A5016258411- Malaria Research and Control
- Insect Resistance and Genetics
- Insect Pest Control Strategies
- Mosquito-borne diseases and control
- Photosynthetic Processes and Mechanisms
- Aquaculture Nutrition and Growth
- Plant nutrient uptake and metabolism
- Physiological and biochemical adaptations
- Forensic Entomology and Diptera Studies
- Microbial Metabolites in Food Biotechnology
- Genetic diversity and population structure
- Fish Biology and Ecology Studies
Atlanta Research and Education Foundation
2006-2010
Centers for Disease Control and Prevention
2007-2009
Centers for Disease Control and Prevention
2007
Abstract Background Polymerase chain reactions to distinguish single-nucleotide polymorphisms are commonly used for mosquito identification and identifying insecticide resistance alleles. However, the existing methods primer design often result in analyses that not robust or require additional steps. Methods Utilizing oligonucleotides unique having an intentional mismatch both templates three bases from SNP at 3-prime end, new PCR assays targets using standard gel electrophoresis of...
ABSTRACT Choline dehydrogenase (EC 1.1.99.1) catalyzes the four-electron oxidation of choline to glycine-betaine via a betaine-aldehyde intermediate. Such reaction is considerable interest for biotechnological applications in that transgenic plants engineered with bacterial glycine-betaine-synthesizing enzymes have been shown enhanced tolerance towards various environmental stresses, such as hypersalinity, freezing, and high temperatures. To date, has poorly characterized its biochemical...
Identification of Anopheles gambiae s.s. and arabiensis from field-collected s.l. is often necessary in basic applied research, operational control programmes. The currently accepted method involves use standard polymerase chain reaction amplification ribosomal DNA (rDNA) the 3' 28S to 5' intergenic spacer region genome, visual confirmation amplicons predicted size on agarose gels, after electrophoresis. This report describes development evaluation an automated, quantitative PCR based upon...
Abstract We present a mosquito marking technique suitable for mark‐release‐recapture that can be used with hand‐held, portable X‐ray fluorescence (XRF) spectrometer, which is practical field measurements. Third instar Anopheles gambiae Giles sensu stricto (Diptera: Culicidae) and stephensi Liston larvae were cultured to pupation in water containing rubidium (Rb) Cl at concentrations up 1000 p.p.m. Rb. survived adulthood as high Rb but suffered pupal mortality reduced adult longevity...
The relationship between mosquito 4th instar larval desiccation and survival to adulthood was explored by three methods in the laboratory. Two colonies of Anopheles arabiensis one gambiae were studied. We found significant differences tolerance among all stocks suggesting an intra- interspecific genetic component tolerance. An. KGB, originating from Zimbabwe about 1975, had a much-reduced compared G3, colonized Gambia DONGOLA which originated Sudan 2004. Individuals G3 stock survived times...
We observed Anopheles gambiae sensu stricto stocks that contained both Mopti (M) and Savanna (S) rDNA intergenic spacers (IGS). ASEMBO1 male IGS sequences consistently had a mixture. A diagnostic M S Hha I restriction enzyme site in these fragments was concordant with two SNPs associated S. Standard demonstrated X-chromosome-only inheritance of the form, but males showed X Y chromosome linkage mixed rDNA. The metaphase chromosomes significantly larger amount DNA relative to than standard...
Abstract Background When rearing morphologically indistinguishable laboratory strains concurrently, the threat of unintentional genetic contamination is constant. Avoidance accidental mixing difficult due to use common equipment, technician error, or possibility self relocation by adult mosquitoes ("free fliers"). In many cases, are distinguish because morphological and similarity, especially when colonies isolates certain traits from same parental strain, such as eye color mutants,...