A5103070177- Advanced Fluorescence Microscopy Techniques
- Renal and related cancers
- Cellular Mechanics and Interactions
- Erythropoietin and Anemia Treatment
- Chronic Kidney Disease and Diabetes
- Renal and Vascular Pathologies
- Extracellular vesicles in disease
- Dialysis and Renal Disease Management
- Cell Image Analysis Techniques
- Microtubule and mitosis dynamics
- Quantum Dots Synthesis And Properties
- Optical Coherence Tomography Applications
- 3D Printing in Biomedical Research
- Gut microbiota and health
- Cell Adhesion Molecules Research
- Clostridium difficile and Clostridium perfringens research
- Inflammatory Bowel Disease
- Mesenchymal stem cell research
- RNA Research and Splicing
- Aortic aneurysm repair treatments
- Eosinophilic Esophagitis
- bioluminescence and chemiluminescence research
- MicroRNA in disease regulation
- Nanocluster Synthesis and Applications
- Single-cell and spatial transcriptomics
Yonsei University
2015-2024
Gangnam Severance Hospital
2014-2024
University of Chicago
2021
Daegu Gyeongbuk Institute of Science and Technology
2021
Hallym University Medical Center
2020
University Health System
2020
Yonsei University Health System
2020
Committee on Climate Change
2020
Chosun University
2012-2017
We recently demonstrated the use of in vitro expanded kidney-derived mesenchymal stem cells (KMSC) protected peritubular capillary endothelial acute renal ischemia-reperfusion injury. Herein, we isolated and characterized microparticles (MPs) from KMSC. investigated their biologic effects on human vivo renoprotective MPs were supernatants KMSC cultured anoxic conditions serum-deprived media for 24 hours. KMSC-derived presence several adhesion molecules normally expressed membranes, such as...
Microparticles (MPs) derived from kidney-derived mesenchymal stem cells (KMSCs) have recently been reported to ameliorate rarefaction of peritubular capillaries (PTC) in ischemic kidneys via delivery proangiogenic effectors. This study aimed investigate whether KMSC-derived MPs show anti-fibrotic effects by ameliorating endothelial-to-mesenchymal transition (EndoMT) human umbilical vein endothelial (HUVEC) vitro and preserving PTC with unilateral ureteral obstruction (UUO) vivo. isolated the...
Recently, a bio-electrochemical technique known as CLARITY was reported for three-dimensional phenotype mapping within transparent tissues, allowing clearer whole-body and organ visualization with CB-perfusion (CUBIC) leading to the development of clearing transparency intact tissues PACT (passive clarity technique) PARS (perfusion-assisted agent release in situ) methodologies. We evaluated structure–function relationships circuits whole central nervous system (CNS) various internal organs...
We propose a novel blood biomarker detection method that uses miRNA super-resolution imaging to enable the early diagnosis of Alzheimer's disease (AD). Here, we report singlemolecule for visualizing disease-specific in tissue from an AD mice model, and peripheral mononuclear cells (PBMCs) patients. Using optimized Magnified Analysis Proteome (MAPs), confirmed five miRNAs contribute neurodegenerative brain hippocampi 5XFAD wild-type mice. also assessed PBMCs isolated whole patients healthy...
Glioblastoma (GBM), one of the most lethal tumors, exhibits a highly infiltrative phenotype. Here, we identified transcription factors (TFs) that collectively modulate invasion-related genes in GBM.The invasiveness tumorspheres (TSs) were quantified using collagen-based 3D invasion assays. TF activities by enrichment analysis GBM transcriptome, and confirmed cell-magnified proteome imaging. Invasion-associated TFs knocked down siRNA or shRNA, TSs orthotopically implanted into mice.After...
Abstract Biological systems consist of a variety distinct cell types that form functional networks. Super-resolution imaging individual cells is required for better understanding these complex systems. Direct visualization 3D subcellular and nano-scale structures in helpful the interpretation biological interactions system-level responses. Here we introduce modified magnified analysis proteome (MAP) method super-resolution (Cell-MAP) which preserves fluorescence. Cell-MAP expands more than...
The advent of tissue clearing methods, in conjunction with novel high-resolution imaging techniques, has enabled the visualization three-dimensional structures unprecedented depth and detail. Although a variety protocols have been developed, little done to quantify their efficacies systematic, reproducible fashion. Here, we present two simple assays, Punching-Assisted Clarity Analysis (PACA)-Light PACA-Glow, which use easily accessible spectroscopy gel documentation systems transparency...
Abstract Recent developments in tissue clearing methods such as the passive technique (PACT) have allowed three-dimensional analysis of biological structures whole, intact tissues, thereby providing a greater understanding spatial relationships and circuits. Nonetheless, issues that remain maintaining structural integrity preventing expansion/shrinkage with rapid still inhibit wide application these techniques hard bone femurs tibias. Here, we present an optimized PACT-based bone-clearing...
Abstract Background Renal tubulointerstitial fibrosis (TIF) plays an important role in the progression of chronic kidney disease (CKD) and its pathogenesis involves epithelial-to-mesenchymal transition (EMT) upon renal injury. Recombinant human erythropoietin (rhEPO) has been shown to display novel cytoprotective effects, part by inhibiting transforming growth factor (TGF)-β1-induced EMT. Here, we evaluated inhibitory effects microparticles (MPs) derived from EPO gene-transfected mesenchymal...
Recent developments in tissue clearing methods have significantly advanced the three-dimensional analysis of biological structures whole, intact tissue, providing a greater understanding spatial relationships and circuits. Nonetheless, studies reported issues with maintaining structural integrity preventing disintegration, limiting wide application these techniques to fragile tissues such as developing embryos. Here, we present an optimized passive technique (PACT)-based embryo method,...
Abstract Tissue biopsy for early diagnosis and monitoring comes with several challenges, such as its invasiveness, issues related to tissue heterogeneity in sampling. To address these issues, researchers have proposed a noninvasive approach called liquid biopsy, which uses blood samples detect specific noncoding RNA (microRNA, miRNA). However, the current process of isolating amplifying miRNA can be time‐consuming yield nonspecific results. In this study, new super‐resolution imaging tool is...
Abstract Three-dimensional visualization of cellular and subcellular-structures in histological-tissues is essential for understanding the complexities biological-phenomena, especially with regards structural spatial relationships pathologlical-diagnosis. Recent advancements tissue-clearing technology, such as Magnified Analysis Proteome (MAP), have significantly improved our ability to study biological-structures three-dimensional space; however, their wide applicability a variety tissues...
Abstract Background: Renal tubulointerstitial fibrosis (TIF) plays an important role in the progression of chronic kidney disease (CKD) and its pathogenesis involves epithelial-mesenchymal transition (EMT) upon renal injury. Recombinant human erythropoietin (rhEPO) has been shown to display novel cytoprotective effects, part by inhibiting transforming growth factor (TGF)-β1-induced EMT. Here, we evaluated inhibitory effects microparticles (MPs) derived from EPO gene-transfected mesenchymal...