A5051509519- Epigenetics and DNA Methylation
- Fibroblast Growth Factor Research
- Nanoparticle-Based Drug Delivery
- Kruppel-like factors research
- Hedgehog Signaling Pathway Studies
- Sports and Physical Education Studies
- Hydrogels: synthesis, properties, applications
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Cancer-related gene regulation
- Advanced Polymer Synthesis and Characterization
- Genetic and Kidney Cyst Diseases
- dental development and anomalies
- Cleft Lip and Palate Research
- 3D Printing in Biomedical Research
- Advanced Drug Delivery Systems
- Circular RNAs in diseases
Charles University
2021-2025
Abstract The FGF signaling pathway plays an important role in the regulation of limb development, controlling cell migration, proliferation, differentiation, and apoptosis. Sprouty proteins act as antagonists control extent part a negative feedback loop. Sprouty2/4 deficient mice evince defects endochondral bone formation digit patterning their forelimbs, with pathogenesis recently related to ciliopathies. To understand mechanisms behind these pathologies, Sprouty2+/−;Sprouty4−/− male female...
Development of dentition is a commonly studied process as representative the development ectodermal derivates. A key step formation signaling center called enamel knot (EK), which organizes tooth crown formation. In mouse lower jaw, anterior part tooth-forming region undergoes series complex events before first molar primary EK can form more posteriorly and progress through cap stage. Although much known about molecular factors involved in development, disentangling their specific roles...
Ectodysplasin (Eda) plays important roles in both shaping the developing tooth and establishing number of teeth within row. Sonic hedgehog (Shh) has been shown to act downstream Eda is involved initiation development. Eda-/- mice possess hypoplastic hypomineralized incisors show changes molar region. In present study we used 3D reconstruction combined with expression analysis, cell lineage tracing experiments, western blot analysis order investigate formation incisor germs mice. We that a...
Aqueous solutions of some polymers exhibit a lower critical solution temperature (LCST); that is, they form phase-separated aggregates when heated above threshold temperature. Such found many promising (bio)medical applications, including in situ thermogelling with controlled drug release, polymer-supported radiotherapy (brachytherapy), immunotherapy, and wound dressing, among others. Yet, despite the extensive research on medicinal applications thermoresponsive polymers, their...
The Sprouty family is a highly conserved group of intracellular modulators receptor tyrosine kinase (RTK)-signaling pathways, which have been recently linked to primary cilia. Disruptions in the structure and function cilia cause inherited disorders called ciliopathies. We aimed evaluate Sprouty2 Sprouty4 gene-dependent alterations ciliary focus on determination its association with Hedgehog signaling defects chondrocytes. Analysis transgenic mice phenotype deficiency revealed several...
In Vivo Biodistribution Thermoresponsive polymers have found many promising (bio)medical applications, e.g. in situ thermogelling with controlled drug-release, polymer-supported brachytherapy, immunotherapy, and wound dressing. Despite the benefits of thermoresponsive polymers, their biodistribution fate remained largely unknown, hindering clinical use. Herein, Ondřej Groborz, Richard Hoogenboom, Martin Hrubý, co-workers elucidate pharmacokinetics mice, article 2201344. Finally, they perform...