A5006515296- dental development and anomalies
- Developmental Biology and Gene Regulation
- Morphological variations and asymmetry
- Genomics and Chromatin Dynamics
- Hippo pathway signaling and YAP/TAZ
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Hedgehog Signaling Pathway Studies
- Ubiquitin and proteasome pathways
- Cleft Lip and Palate Research
- Genomics and Phylogenetic Studies
- Bone and Dental Protein Studies
- Neurobiology and Insect Physiology Research
- Biotechnology and Related Fields
- Plant Molecular Biology Research
- Cancer-related Molecular Pathways
- Science, Research, and Medicine
- RNA Research and Splicing
- Infectious Diseases and Mycology
- Sexual Differentiation and Disorders
- NF-κB Signaling Pathways
- Invertebrate Immune Response Mechanisms
- Cellular Mechanics and Interactions
- Single-cell and spatial transcriptomics
- Connective tissue disorders research
École Normale Supérieure de Lyon
2014-2025
Inserm
2016-2025
Université Claude Bernard Lyon 1
2014-2025
Centre National de la Recherche Scientifique
2014-2025
Laboratoire de Biologie et Modélisation de la Cellule
2016-2023
Institut de Génomique Fonctionnelle de Lyon
2007-2019
Centre de Biologie du Développement
2002-2003
Institut de Biologie Valrose
2003
Centre Antoine Lacassagne
2003
Université Côte d'Azur
2003
It is known from paleontology studies that two premolars have been lost during mouse evolution. During mandible development, bud-like structures transiently form may represent rudimentary precursors of the premolars. However, interpretation these and their significance for molar development are highly controversial because a lack molecular data. Here, we searched typical tooth signaling centers in structures, followed fate using markers, 3D reconstructions, lineage tracing vitro. Transient...
Abstract Background The development of the secondary palate has been a main topic in craniofacial research, as its failure results cleft palate, one most common birth defects human. Nevertheless, palatal rugae (or palatinae ), which are transversal ridges developing on received little attention. However, could be useful landmarks to monitor anterior/posterior (A/P) growth, and they provide simple model mesenchymal-epithelial structures arranged serial pattern. Results We first determined...
Significance Good examples of the relative autonomy organ development are teeth, and for decades researchers have cultured isolated mouse teeth on a petri dish by starting from earliest recognizable stage. We cheek voles, another species rodent, show how vole lack their species-specific morphology. Based computational modeling, 3D imaging, experiments in which we culture between microscopic braces, suggest that tooth shape requires lateral support jaw. Because mice vole-specific morphology,...
Mice have evolved a new dental plan with two additional cusps on the upper molar, while hamsters were retaining ancestral plan. By comparing dynamics of molar development transcriptome time series, we found at least three early changes in mouse development. Together, they redirect spatio-temporal to ultimately form cusps. The lower has undergone much more limited phenotypic evolution. Nevertheless, its developmental trajectory as that and co-evolved it. Among coevolving changes, some are...
ABSTRACT Although it is well established that certain stages of development are more conserved than others, the reasons for this phenomenon remain largely unknown. We study molecular conservation in an organ, molar, by comparing temporal profiles expression mice and hamsters. find molar characterized a rarely observed pattern level coding sequences forming inverse hourglass, with at beginning end morphogenesis intermediate stages. As rodent described, we were able to link properties...
Development of dentition is a commonly studied process as representative the development ectodermal derivates. A key step formation signaling center called enamel knot (EK), which organizes tooth crown formation. In mouse lower jaw, anterior part tooth-forming region undergoes series complex events before first molar primary EK can form more posteriorly and progress through cap stage. Although much known about molecular factors involved in development, disentangling their specific roles...
When patterns are set during embryogenesis, it is expected that they straightly established rather than subsequently modified. The patterning of the three mouse molars is, however, far from straight, likely as a result evolutionary history. first-formed tooth signaling centers, called MS and R2, disappear before driving formation thought to be vestiges premolars found in ancestors. Moreover, mature center first molar (M1) formed fusion two centers (R2 early M1). Here, we report broad...
It is widely accepted that evolutionary changes in conserved developmental signaling pathways play an important role morphological evolution. However, few silico studies were interested tracking such a pathway. The Ectodysplasin (EDA) pathway provides opportunity to fill this gap because it involved vertebrate skin appendage development as scales, teeth, hair, and feathers take obvious part the adaptation of species their environment. We benefited from large amount genomic data now available...
The Eda-A1-Edar signaling pathway is involved in the development of organs with an ectodermal origin, including teeth. In mouse, mutants are known for both ligand, Eda-A1 (Tabby), and receptor, Edar (Downless). adult dentitions these two have classically been considered to be similar. However, previous studies mentioned differences embryonic dental between Eda(Ta) Edar(dl-J) mutants. A detailed study tooth morphology bearing losses functions genes thus appears necessary test pattern...
Variation within a population is key feature in evolution, because it can increase or impede response to selection, depending on whether not the intrapopulational variance correlated change under selection. Hence, main directions of genetic have been proposed constitute "lines least resistance evolution" along which evolution would be facilitated. Yet, screening selection occurs at phenotypic level, and only product underlying variance, but also developmental processes. It thus issue for...
Abstract The Tabby/eda mice, which bear a loss of function mutation for the eda (ectodysplasinA) gene, are known to display developmental anomalies in organs with an ectodermal origin. Although lower jugal (cheek) teeth mice have been extensively studied, upper never investigated detail. However, this may help us further understand gene tooth development. In work, shape and size both crown radicular system were studied teeth. To deal high morphological variability, we defined several...
SUMMARY Morphological integration corresponds to interdependency between characters that can arise from several causes. Proximal causes of include different phenotypic features may share common genetic sets and/or interact during their development. Ultimate be the prolonged effect selection favoring functionally interacting characters, achieved by molding these proximal Strong and direct interactions among successive teeth a molar row are predicted developmental evidences. Functional...
Comparative transcriptomics can answer many questions in developmental and evolutionary biology. Most transcriptomic studies start by showing global patterns of variation transcriptomes that differ between species or organs through time. However, little is known about the kinds expression differences shape these patterns. We compared during development two morphologically distinct serial organs, upper lower first molars mouse. found types teeth largely share same gene dynamics but three...
Do developmental systems preferentially produce certain types of variation that orient phenotypic evolution along preferred directions? At different scales, from the intra-population to interspecific, murine first upper molar shows repeated anterior elongation. Using a novel quantitative approach compare development two mouse strains with short or long molars, we identified temporal, spatial and functional differences in tooth signaling center activity, arise differential tuning...
Abstract Multicellular organisms grow through both proliferation and growth of their individual cells. We have conducted a P-element-based misexpression screen for genes whose upregulation alters wing disc during development. One particular group four P elements, all inserted at cytological location 61C7-8, exhibited specific overgrowth upon in proliferating imaginal tissues. Clonal analysis revealed that misexpression, cell number was increased but size not affected, indicating were induced...
Metazoans are largely made of repeated parts, and metazoan evolution is marked by changes in the number these called meristic evolution. Understanding mechanisms associated with thus a critical issue to evolutionary developmental biology. Palatal rugae sensory ridges regularly arranged on hard palate mammals. They develop sequentially following mesio-distal growth palate, activation-inhibition very likely control spacing timing this sequential addition. In study, we characterized trends...
Numerous loci of large effect have been shown to underlie phenotypic variation between species. However, with subtle effects are presumably more frequently involved in microevolutionary processes but rarely discovered. We explore the genetic basis shape first upper molar hybrid mice Mus musculus and M. m. domesticus. performed genome-wide association study for used 3D surface morphometrics quantify individuals. show that many small variation, identify five genomic regions associated tooth...
Only a handful of signaling pathways are major actors development and responsible for both the conservation diversification animal morphologies. To explain this twofold nature, gene duplication enhancer evolution were predominantly put forth as tinkering mechanisms whereas alternative isoforms has been, so far, overlooked. We investigate here role gain loss using Edaradd, Ecodysplasin pathway, implicated in morphological evolution. A previous study had suggested scenario isoform with an (A)...