A5082748146- Orthopedic Surgery and Rehabilitation
- Bone fractures and treatments
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Cancer Diagnosis and Treatment
- Shoulder Injury and Treatment
- Total Knee Arthroplasty Outcomes
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- Knee injuries and reconstruction techniques
- CAR-T cell therapy research
- Shoulder and Clavicle Injuries
- Congenital Diaphragmatic Hernia Studies
- Genital Health and Disease
- Foot and Ankle Surgery
- Dupuytren's Contracture and Treatments
- Pelvic and Acetabular Injuries
- Gestational Trophoblastic Disease Studies
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- T-cell and B-cell Immunology
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Hospices Civils de Lyon
2015-2025
Université Claude Bernard Lyon 1
2018-2024
Hôpital Lyon Sud
2015-2024
University Dermatology
2022
Centre National de la Recherche Scientifique
2018-2019
Centre Léon Bérard
2019
Inserm
2018-2019
Centre de Recherche en Cancérologie de Lyon
2018-2019
Hôpital d'instruction des Armées Desgenettes
2012-2014
Research Article5 September 2016Open Access Source DataTransparent process ZEB1-mediated melanoma cell plasticity enhances resistance to MAPK inhibitors Geoffrey Richard Cancer Center of Lyon, INSERM U1052, France CNRS UMR 5286, Université de ISPB, Lyon 1, Centre Léon Bérard, Search for more papers by this author Stéphane Dalle Dermatology Unit, Hospices Civils CH Sud, Pierre Bénite Cedex, Marie-Ambre Monet Maud Ligier Amélie Boespflug Roxane M Pommier Arnaud la Fouchardière Department...
Background The efficacy of immunotherapies in metastatic melanoma depends on a robust T cell infiltration. Oncogenic alterations tumor cells have been associated to exclusion. Identifying novel cancer cell-intrinsic non-genetic mechanisms immune escape, the targeting which would reinstate recruitment, allow restore response anti-programmed death protein 1 (PD-1) antibody therapy. epithelial-to-mesenchymal transition (EMT)-inducing transcription factor ZEB1 is major regulator plasticity,...
Abstract Dendritic cells (DCs) are promising targets for cancer immunotherapies because of their central role in the initiation and control immune responses. The rare cDC1 population is particular interest its remarkable ability to cross-present antigens (Ag) CD8+ T cells, promote Th1 cell polarization NK activation recruitment. However, spatial organization specific functions cDC1s response immunotherapy remain be clearly characterized human tumors. Here, we implemented a multiplexed...
Tumor cells can evade antitumor immune response by expressing the PD-L1 ligand, leading to inhibition of PD-1-expressing T lymphocytes. The mechanisms that regulate expression in cancer are imperfectly characterized. transcription factor ZEB1, a major regulator phenotype switching melanoma cells, was shown promote escape repressing cell infiltration. Using inducible models and ZEB1 gain/loss-of-function melanoma, we show binds CD274 (PD-L1) promoter, directly enhancing mRNA its at membrane....
<div>Abstract<p>Dendritic cells (DC) are promising targets for cancer immunotherapies because of their central role in the initiation and control immune responses. The type 1 conventional DC (cDC1) population is particular interest its ability to cross-present antigens CD8<sup>+</sup> T cells. cDC1s also secrete cytokines that allow Th1 cell polarization NK activation recruitment. However, spatial organization specific functions response immunotherapy remain be...
<p>Optimized immunofluorescence staining conditions for the multiplex panel</p>
<p>Description of the MELPREDICT cohort and design experiments performed in study.</p>
<p>Clinical features of MELPREDICT patients at baseline prior immunotherapy onset</p>
<p>Composition of DC aggregates and frequency in R NR patients.</p>
<p>Work flow analysis for the multiplexed immunofluorescence staining.</p>
Whereas immunotherapies have revolutionized the treatment of different solid and hematological cancers, their efficacy in nodal peripheral T-cell lymphomas (PTCLs) is limited, due to a lack understanding immune response they trigger. To fully characterize tumor microenvironment (TME) PTCLs, we performed spectral flow cytometry analyses on 11 angioimmunoblastic (AITL), 7 PTCL, not otherwise specified (PTCL, NOS) lymph node samples, 10 non-tumoral control samples. The PTCL TME contained larger...
Abstract Background Dendritic cells (DCs) are promising targets for cancer immunotherapies owing to their central role in the initiation and control of immune responses. Their functions encompass a wide range mechanisms mediated by different DC subsets. Several studies have identified human tumor- associated (TA-DC) populations through limited marker-based technologies, such as immunostaining or flow cytometry. However, tumor infiltration, spatial organization specific response immunotherapy...
Background.Some cutaneous melanomas are arising from nevus, that can be either the congenital or common type.Objective: We aimed at comparing: i) genomic landscape of small/medium versus nevi and ii) explore whether there is a different mutational pattern acquired during progression to melanoma between this two groups. Methods:We analysed BRAF, NRAS TERT promoter status on micro-dissected melanocytic performed massively parallel sequencing paired in 42 patients, half type.Results: Both...