A5028001713- Effects and risks of endocrine disrupting chemicals
- Physiological and biochemical adaptations
- Carcinogens and Genotoxicity Assessment
- Aquatic Ecosystems and Phytoplankton Dynamics
- Hemoglobin structure and function
- Animal testing and alternatives
- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- Genetically Modified Organisms Research
- Environmental Toxicology and Ecotoxicology
- High Altitude and Hypoxia
- Chemistry and Chemical Engineering
- Anesthesia and Neurotoxicity Research
- Biomedical Ethics and Regulation
- Animal Nutrition and Physiology
- Animal Behavior and Welfare Studies
- Primate Behavior and Ecology
- Pharmaceutical studies and practices
- Meat and Animal Product Quality
- Inhalation and Respiratory Drug Delivery
- Erythrocyte Function and Pathophysiology
- Hemoglobinopathies and Related Disorders
- Animal Behavior and Reproduction
- Animal Vocal Communication and Behavior
- Occupational exposure and asthma
Federal Institute for Risk Assessment
2015-2024
European Food Safety Authority
2014-2016
German Primate Center
2009-2013
University of Münster
2003-2012
Humboldt-Universität zu Berlin
1994-1995
Abstract The need to develop a blood substitute is now urgent because of the increasing concern over Europe's BSE outbreak and worldwide HIV/AIDS epidemic, which have cut supplies. Extracellular soluble hemoglobin has long been studied for its possible use as safe effective alternative transfusion, but this met with little success. Clinical trials revealed undesirable side effects–oxidative damage vasoconstriction–that hamper application cellfree substitute. We addressed these problems found...
Abstract Background Temperature affects essentially every aspect of the biology poikilothermic animals including energy and mass budgets, activity, growth, reproduction. While thermal effects in ecologically important groups such as daphnids have been intensively studied at ecosystem level least partly organismic level, much less is known about molecular mechanisms underlying acclimation to different temperatures. By using 2D gel electrophoresis spectrometry, present study identified major...
Recently revised OECD Testing Guidelines highlight the importance of considering first site-of-contact when investigating genotoxic hazard. Thus far, only in vivo approaches are available to address dermal route exposure. The 3D Skin Comet and Reconstructed Micronucleus (RSMN) assays intend close this gap vitro genotoxicity toolbox by DNA damage after topical application. This represents most relevant exposure for a variety compounds found household products, cosmetics, industrial chemicals....
As part of the safety assessment process, all industrial sectors employ genotoxicity test batteries, starting with well-established in vitro assays. However, these batteries have limited predictive capacity for vivo situation, which may result unnecessary follow-up testing or loss promising substances where animal tests are prohibited not desired. To address this, a project involving regulators, academia and industry was established to develop validate human skin-based assays topically...
To determine the contribution of haemoglobin (Hb) to hypoxia-tolerance Daphnia magna, we exposed Hb-poor and Hb-rich individuals (2.4-2.8 mm long) a stepwise decrease in ambient oxygen partial pressure (P(O(2)amb)) over period 51 min from normoxia (20.56 kPa) anoxia (<0.27 looked for differences their physiological performance. The haem-based concentrations Hb haemolymph were 49 micromol l(-1) 337 animals, respectively. experimental apparatus made simultaneous measurement appendage beating...
Abstract Background Freshwater planktonic crustaceans of the genus Daphnia show a remarkable plasticity to cope with environmental changes in oxygen concentration and temperature. One key proteins adaptive gene control pulex under hypoxia is hemoglobin (Hb), which increases hemolymph by an order magnitude shows enhanced affinity due subunit composition. To explore full spectrum protein expression response low-oxygen conditions, two-dimensional gel electrophoresis mass spectrometry were used...
Gaze-following, the tendency to direct one's attention locations looked at by others, is a crucial aspect of social cognition in human and nonhuman primates. Whereas development gaze-following has been intensely studied infants, its early ontogeny primates received little attention. Combining longitudinal cross-sectional observational data from Barbary macaques 'La Forêt des Singes', we show here that among conspecifics develops within first year life with rapid increase between 5 6 months,...
We have developed a new in vitro skin irritation test based on an open source reconstructed epidermis (OS-REp) with openly accessible protocols for tissue production and performance. Due to structural, mechanistic procedural similarity, blinded catch-up validation study according OECD Performance Standards (PS) was conducted three laboratories promote regulatory acceptance, OS-REp models produced at single site only. While overall sensitivity predictive capacity met the PS requirements,...
Human exposures to bisphenol A (BPA) are widespread. The current study addresses uncertainties regarding human pharmacokinetics of BPA following dermal exposure.To examine the absorption, distribution, metabolism and excretion in humans administration.We dermally administered deuterated (d6-BPA) 10 subjects (6 men 4 women) at a dose 100 µg/kg over 12-hour period conducted blood urine analysis from beginning dosing through three- or six-day period. We present time-course serum concentrations...
Recent studies on Daphnia magna have revealed that the feeding current is important for uptake of oxygen from ambient medium. Respiratory gas exchange should therefore mainly occur within filtering chamber, whose boundaries are formed by trunk and extended carapace shell valves. The precise site in genus is, however, a matter conjecture. We developed method imaging haemoglobin oxygen-saturation circulatory system transparent animals, which provides an opportunity to localize environment...
In acute inhalation toxicity studies, animals inhale substances at given concentrations. Without additional information, however, appropriate starting concentrations for in-vivo studies are difficult to estimate. The goal of this project was the prevalidation precision-cut lung slices (PCLS) as an ex-vivo alternative reduce number used in studies. According internationally agreed principles Prevalidation Studies, conducted three independent laboratories. German BfR provided consultancy...
SUMMARY The mechanisms, dynamics and effects of hypoxia acclimation were studied in the water flea Daphnia magna over three successive generations(parental, first second filial generation: P, F1 F2). P generation was raised under normoxic conditions at 20°C became exposed to environmental (10-19% air saturation)at maturity. Their progenies (F1 F2) experienced from birth onwards. Controls kept conditions. Individuals successively sampled a 3-day interval each group determine...
Acidity exerts a determining influence on the composition and diversity of freshwater faunas. While physiological implications acidification have been intensively studied in teleost fish crayfish, much less is known about acid-stress physiology ecologically important groups such as cladoceran zooplankton. This study analyzed extracellular acid-base state CO2 partial pressure (PCO2), circulation ventilation, well respiration rate Daphnia pulex acclimated to acidic (pH 5.5 6.0) circumneutral...