A5068907124- Nanoparticles: synthesis and applications
- Pluripotent Stem Cells Research
- Sphingolipid Metabolism and Signaling
- Protein Kinase Regulation and GTPase Signaling
- Cellular transport and secretion
- 3D Printing in Biomedical Research
- Lysosomal Storage Disorders Research
- Effects and risks of endocrine disrupting chemicals
- Animal testing and alternatives
- Erythrocyte Function and Pathophysiology
- Chemistry and Chemical Engineering
- Air Quality and Health Impacts
- Cell death mechanisms and regulation
- Lipid Membrane Structure and Behavior
- Anesthesia and Neurotoxicity Research
- Biomedical Ethics and Regulation
- Graphene and Nanomaterials Applications
- Lipid metabolism and biosynthesis
- Computational Drug Discovery Methods
- Pharmacological Effects and Toxicity Studies
- Advanced biosensing and bioanalysis techniques
- Bone health and treatments
- Recycling and Waste Management Techniques
- Carcinogens and Genotoxicity Assessment
- Heavy Metal Exposure and Toxicity
Federal Institute for Risk Assessment
2013-2024
Albert Einstein College of Medicine
2006-2009
Yeshiva University
2009
Weizmann Institute of Science
2002-2004
Freie Universität Berlin
1998-2003
National Institutes of Health
2003
Deutsche Forschungsgemeinschaft
2003
University Medical Center
2001
Overexpression of upstream growth and differentiation factor 1 (uog1), a mammalian homolog the yeast longevity assurance gene (LAG1), selectively induces synthesis stearoyl-containing sphingolipids in cells (Venkataraman, K., Riebeling, C., Bodennec, J., Riezman, H., Allegood, J. Sullards, M. Merrill, A. H. Jr., Futerman, (2002) Biol. Chem. 277, 35642-35649). Gene data base analysis subsequently revealed new subfamily proteins containing Lag1p motif, previously characterized as translocating...
The longevity assurance gene (LAG1) and its homolog (LAC1) are required for acyl-CoA-dependent synthesis of ceramides containing very long acyl chain (e.g. C26) fatty acids in yeast, a LAG1, ASC1, confers resistance plants to fumonisin B1, an inhibitor ceramide synthesis. To understand further the mechanism regulation synthesis, we now characterize mammalian LAG1,upstream growth differentiation factor-1 (uog1). cDNA clones uog1 were obtained from expression sequence-tagged sub-cloned into...
We recently demonstrated that elevation of intracellular glucosylceramide (GlcCer) levels results in increased functional Ca2+ stores cultured neurons, and suggested this may be due to modulation ryanodine receptors (RyaRs) by GlcCer (Korkotian, E., Schwarz, A., Pelled, D., Schwarzmann, G., Segal, M. Futerman, A. H. (1999) J. Biol. Chem. 274, 21673–21678). now systematically examine the effects exogenously added GlcCer, other glycosphingolipids (GSLs) their lyso-derivatives on release from...
Gangliosides are found at high levels in neuronal tissues where they play a variety of important functions. In the gangliosidoses, gangliosides accumulate because defective activity lysosomal proteins responsible for their degradation, usually resulting rapidly progressive neurodegenerative disease. However, molecular mechanism(s) leading from ganglioside accumulation to neurodegeneration is not known. We now examine effect GM2 mouse model Sandhoff disease (one gangliosidoses), Hexb–/–...
Treatment of cultured hippocampal neurons with high concentrations short-chain acyl ceramide derivatives, such as N-hexanoyl-D-sphingosine (C(6)-Cer), results in apoptotic cell death. We now show that death-associated protein (DAP) kinase plays an important role mediating this effect. Upon incubation C(6)-Cer, DAP levels are elevated early 1 h after treatment, reaching 2-3-fold higher than untreated cells 4 h. Neurons from kinase-deficient mice were significantly less sensitive to apoptosis...
To keep pace with its rapid development an efficient approach for the risk assessment of nanomaterials is needed. Grouping concepts as developed chemicals are now being explored applicability to nanomaterials. One recently proposed grouping systems DF4nanoGrouping scheme. In this study, we have three structure-activity relationship classification tree models be used supporting system by identifying structural features mainly responsible surface activity. We data from 19 that were synthesized...
Nanotechnology risk management strategies and environmental regulations continue to rely on hazard exposure assessment protocols developed for bulk materials, including larger size particles, while commercial application of nanomaterials (NMs) increases. In order support corroborate NMs workers, consumers, the environment it is crucial establish impact biopersistence at realistic doses. future, such data will allow a more refined categorization NMs. Despite many experiments NM...
We examined the biological effects of ceramide analogues (1 S ,2 R )‐2‐ N ‐myristoylamino‐1‐phenyl‐1‐propanol ( D ‐ e ‐MAPP) and ‐myristoylamino‐1‐(4‐nitrophenyl)‐1,3‐propandiol ‐NMAPPD) on human HaCaT keratinocytes melanoma cells. could demonstrate that ‐MAPP ‐NMAPPD are able to suppress acid ceramidase activity. The elevation endogenous level is followed by induction apoptosis suppression proliferation in keratinocytes. Moreover, we recently identified a group cell populations which...
Oxidative stress, a commonly used paradigm to explain nanoparticle (NP)-induced toxicity, results from an imbalance between reactive oxygen species (ROS) generation and detoxification. As one consequence, protein carbonyl levels may become enhanced. Thus, the qualitative quantitative description of carbonylation be characterize how biological systems respond oxidative stress induced by NPs. We investigated representative panel 24 NPs including functionalized amorphous silica (6), zirconium...
Characterization of carbon nanotube dispersions requires measurement both, concentration and surface area.
The project nanoGRAVUR (BMBF, 2015-2018) developed a framework for grouping of nanomaterials. Different groups may result each the three distinct perspectives occupational, consumer and environmental safety. properties, methods descriptors are harmonised between based on: Tier 1 intrinsic physico-chemical properties (what they are) or GHS classification non-nano-form (human tox, ecotox, physical hazards); 2 extrinsic release from nano-enabled products, in vitro assays with cells (where go;...
Glucosylceramide (GlcCer) accumulates in the inherited metabolic disorder, Gaucher disease, because of defective activity lysosomal glucocerebrosidase. We previously demonstrated that upon GlcCer accumulation, cultured hippocampal neurons exhibit modified growth patterns, altered endoplasmic reticulum density, and calcium release from intracellular stores. here examined relationship between accumulation phospholipid synthesis. After treatment with an active site-directed inhibitor...
Pamidronate belongs to the class of nitrogen-containing bisphosphonates that are potent inhibitors bone resorption frequently used for treatment osteoporosis and cancer-induced osteolysis. The inhibition osteoclasts' growth has been suggested as main mechanism inhibitory effect pamidronate on metastases. Recent findings indicated also have a direct apoptotic other types tumour cells. Nitrogen-containing were shown inhibit farnesyl diphosphate synthase, thus blocking synthesis higher...
In biomedical research, the past two decades have seen advent of in vitro model systems based on stem cells, humanized cell lines, and engineered organotypic tissues, as well numerous cellular assays primarily established tumor-derived lines their genetically modified derivatives.There are high hopes that these might replace need for animal testing regulatory toxicology. However, despite increasing pressure recent years to reduce testing, regulators still reluctant adopt approaches a large...