A5090473450- Nanoparticles: synthesis and applications
- Advanced Glycation End Products research
- Metal and Thin Film Mechanics
- Chemistry and Chemical Engineering
- Vibrio bacteria research studies
- Nanotechnology research and applications
- Recycling and Waste Management Techniques
- Enzyme Structure and Function
- Copper-based nanomaterials and applications
- ZnO doping and properties
- Bone Tissue Engineering Materials
- Microplastics and Plastic Pollution
- Escherichia coli research studies
- Cassava research and cyanide
- Natural Antidiabetic Agents Studies
- Nicotinic Acetylcholine Receptors Study
- Nanomaterials for catalytic reactions
- DNA Repair Mechanisms
- Phytochemicals and Antioxidant Activities
- Erythrocyte Function and Pathophysiology
- Polymer Surface Interaction Studies
- Polysaccharides Composition and Applications
- Carcinogens and Genotoxicity Assessment
- GABA and Rice Research
- Orthopaedic implants and arthroplasty
Institute of Molecular Biology
2005-2024
Bulgarian Academy of Sciences
2011-2024
Summary We have recently shown that the process of non‐enzymatic glycosylation (glycation) takes place in Escherichia coli under physiological conditions and affects both recombinant endogenous bacterial proteins. In this study, we further demonstrate E. chromosomal DNA is also subjected to glycation growth conditions. The accumulates early (Amadori) products as proven by nitroblue tetrazolium (NBT) reduction assay. It showed immunoreactivity a monoclonal antibody raised against N ∈...
This work presents results on the influence of thickness structure and biological response Cu-O coatings deposited commercially pure titanium (cpTi) substrates using direct current (DC) magnetron sputtering. The deposition times were 5, 10, 15 min to obtain with different thicknesses. show that films for correspond thicknesses 41, 74, 125 nm, respectively. phase composition is in form a double-phase CuO Cu2O all considered cases. roughness nanometric scale no obvious trend as function can be...
One of the primary risk factors for implant failure is thought to be implant-related infections during early healing phase. Developing coatings with cell stimulatory behaviour and bacterial adhesion control still difficult bone implants. This study proposes an approach one-step deposition biocompatible antimicrobial Cu-doped TiO2 via glow-discharge sputtering a mosaic target. During deposition, bias Ti6Al4V substrates was changed. Structure examination, phase analysis, surface morphology...
Abstract The antimicrobial activity of nanoparticles (NPs) depends the surface area in contact with microorganisms. large enhances their interaction microbes. In this work, a green, simple, rapid, and efficient ultrasound-assisted reduction method for silver (AgNP) synthesis is presented. For synthesis, an aqueous solution nitrate, ethanol, ammonia was used. adopted can be easily implemented any kind scientific or industrial application due to its cost-effective nature. effect sonication...
Inhibitors of acetylcholinesterase (AChE) are used to treat many disorders, among which neurodegenerative upsets, like Alzheimer's disease (AD). One the limited licensed AChE inhibitors (AChEIs) as drugs is natural compound galantamine (Gal).As Gal a toxic compound, here we expose data about its four derivatives in hybrid peptide-norgalantamine molecules, have shown 100 times lower toxicity.Four newly synthesized been involved docking analysis made by Molegro Virtual Docker. Biological...
Abstract Copper has gained increased interest because of its important role in metabolism and antimicrobial activity. In this study, composite target material Ti Cu was used to deposit TiO 2 coatings doped with copper on Ti6Al4V alloy. The aim examine the influence applied bias during deposition Cu-doped coating by sputtering a glow-discharge pure O atmosphere for time 240 min. Different substrate values, selected from 0 -150 V, were process. increase voltage -50 - 150 V decreased thickness...
Early biofilm formation could be inhibited by applying a thin biocompatible copper coating to reduce periprosthetic infections. In this study, we deposited crystalline Cu-doped TiO2 films using one-step DC magnetron sputtering in an oxygen atmosphere on biased Ti6Al4V alloy without external heating. The bias voltage varied from −25 V −100 V, and the resultant substrate temperature was measured. coatings were characterized X-ray diffraction (XRD), scanning electron microscopy (SEM),...
Natural ingredients rich in antioxidants and polyphenols the forme of extracts, essential oils, organic acids have been used production different cosmetic products. In development new compositions, or improvement products already implemented production, evaluation user is great importance, as it directly affects demand for a given product. The present study aimed to produce phytocosmetic creams based on stearic acid with extracts dry flower fruit juice Sambucus nigra L. (S. nigra)...
ABSTRACTABSTRACTRecently, we have shown that the process of non-enzymatic glycosylation (glycation) affects Escherichia coli chromosomal DNA under physiological conditions in vivo. We further demonstrated glycation is a source spontaneous mutations E. coli. The present study reveals inhibition does not necessarily lead to suppression mutability. tested effect acetyl salicylic acid (ASA) on and mutagenesis At concentration 0.1 mM growth medium ASA proved be non-toxic inhibits significantly...
Glycation is a spontaneous chemical reaction, which affects the structure and function of proteins under normal physiological conditions. Therefore, organisms have evolved diverse mechanisms to combat glycation. In this study, we show that Escherichia coli glycolytic enzyme phosphoglucose isomerase (Pgi) exhibits deglycation activity. We found E. Pgi catalyzes breakdown glucose 6-phosphate (G6P)-derived Amadori products (APs) in chicken lysozyme. The affinity glycated lysozyme (K
Amadoriases are discovered in distant groups of organisms ranging from bacteria to humans. These enzymes catalyze the degradation Amadori products which formed early stage a non-enzymatic reaction between reducing sugars and primary amines called Maillard or glycation. The physiological role amadoriases is debatable perhaps not constrained one cellular process only. Among other functions proposed ensure enzymatic defense against deleterious consequences protein A decade ago an amadoriase...
Phosphoglucose isomerase (PGI) is a key enzyme in early glycolysis, which catalyzes the reversible isomerization of glucose 6-phosphate (G6Ph) to fructose 6-phosphate. We have constructed an Escherichia coli K12 strain with deleted pgi gene (Δpgi) and shown that this comparison parental 1) accumulates higher amount G6Ph, 2) grows slowly, 3) exhibits spontaneous mutation frequency rifampicin resistance (Rifr), when grown on high minimal medium. Intriguingly, rate Rifr was inversely related...