A5066328182- Enzyme Structure and Function
- Crystallization and Solubility Studies
- Chemical synthesis and alkaloids
- Nicotinic Acetylcholine Receptors Study
- Chemical Synthesis and Analysis
- nanoparticles nucleation surface interactions
- Cholinesterase and Neurodegenerative Diseases
- Supramolecular Self-Assembly in Materials
- Synthesis and biological activity
- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Renal Diseases and Glomerulopathies
- Computational Drug Discovery Methods
- Protein Structure and Dynamics
- Biochemical effects in animals
- Biochemical Analysis and Sensing Techniques
- Tryptophan and brain disorders
- Lipid Membrane Structure and Behavior
- Iron Metabolism and Disorders
- Trace Elements in Health
- Photochromic and Fluorescence Chemistry
- Microscopic Colitis
- Biomedical Research and Pathophysiology
- Plant Molecular Biology Research
- Ginseng Biological Effects and Applications
University of Chemical Technology and Metallurgy
2007-2024
Sofia University "St. Kliment Ohridski"
2005-2019
Imperial College London
2012
Universitat Jaume I
2007
Bulgarian Academy of Sciences
1997-2006
Institute of Physical Chemistry
2001
Research Institute of Human Morphology
1997
The food industry is interested in replacing artificial sweeteners with natural sugars that possess zero calories and carbohydrates do not cause spikes blood sugar levels. steviosides leaves, synthesized at Stevia rebaudiana Bertoni, are 300 times sweeter than common table sugar. propagation limited due to the poor viability of seeds, long time low germination rate, rooting ability vegetative cuttings. Because this, an alternative biotechnological method for its reproduction being studied,...
Alzheimer’s disease (AD), a progressive neurodegenerative disorder characterized by memory loss and cognitive functions decline, is leading cause for dementia currently ranked as the sixth foremost of death. As present, treatment AD symptomatic without convincing therapeutic benefits new, effective, agents are pursued. Due to massive cholinergic neurons decreased acetylcholine levels, cholinesterase inhibitors like galantamine, remain backbone pharmacological disease. In present study, using...
The heterogeneous nucleation of hen-egg-white lysozyme (HEWL) crystals has been repeatedly investigated using a double-(thermal)-pulse technique, thus detaching from growth stage. n(t) dependencies the nucleus number n, on templates poly-L-lysine, vs time, t were plotted and steady-state rates I determined. They compared with results obtained earlier for surfaces rendered hydrophobic (by means hexamethyl-disilazane) as well bare glass surfaces. In present paper we determine HEWL molecules in...
The formation of self-assembled fibrillar networks in the absence solvent is reported for a whole family amino acid derived gelators containing nicotinoyl and isonicotinoyl terminal groups. Fibrillar can be formed easily by sublimation process (vapor–solid method). In this work an unprecedented comparison microfibrillar structures organogelators within (acetonitrile toluene) solvent. use different techniques such as scanning electron microscopy (SEM), wide-angle X-ray diffraction or FT-IR...
The study of the crystalline xerogel formed by a newly synthesized l-valine and pyridine containing low-molecular-weight gelator reveals polymorphic transition above 225 °C that is followed formation microfibers. habitus fibers grown remains stable upon cooling. Microscopic observations lead to suggestion after transition, are sublimation process. Results obtained reveal intrinsic tendency this compound self-assemble anisotropically in permit preparation organic micro- nanofibers...
The nucleation of horse spleen ferritin (HSF) crystals on substrates was investigated using a new modification the double pulse technique. influence three different structureless * (glass, glass covered by methyl groups and poly-L-lysin template) studied. boundaries in phase-diagram, which separate zones crystal growth were obtained keeping pH = 5.0, CdSO 4 as crystallizing agent. steady-state rates determined. energy required for critical nuclei formation evaluated (10 -13 erg) sizes found...
Although no effective treatment for the Alzheimer's disease currently exist, some drugs acting as Acetylcholinesterase inhibitors, like galanthamine have positively affected such patients. β- and/or γ-secretase inhibitors are another type of potential drugs. Here we report synthesis new peptide-galanthamine derivatives, with expected inhibitory activity against both and β-secretase.The aim this work is obtaining peptide derivatives decreased toxicity compared to galanthamine.Syntheses were...
Inhibitors of acetylcholinesterase (AChE) are used to treat many disorders, among which neurodegenerative upsets, like Alzheimer's disease (AD). One the limited licensed AChE inhibitors (AChEIs) as drugs is natural compound galantamine (Gal).As Gal a toxic compound, here we expose data about its four derivatives in hybrid peptide-norgalantamine molecules, have shown 100 times lower toxicity.Four newly synthesized been involved docking analysis made by Molegro Virtual Docker. Biological...
The use of nanomaterials in biotechnology for the vitro propagation medical plants and accumulation certain biologically active metabolites is becoming an efficient strategy. This study aimed to evaluate influence concentration (0, 1, 10, 50, 100 mg L-1) two types nanofibers on growth characteristics, antioxidant status, production steviol glycosides micropropagated Stevia rebaudiana Bert. plantlets. were synthesized by aspartic acid derivatives (L-Asp) Ag salts self-organized into with...
The degree of adhesion protein crystals, heterogeneously nucleated and grown on different supports (e.g. glass plates coated with poly-L-lysine, hexamethyl-disilazane silicon) is measured directly a purposely-developed technique. sticking force crystal/support determined by means flexible fibre, which bending calibrated series weights. In this way an elastic constant, specific for each fiber individually. Appropriate fibres relative less than 10% (Hook's law) are used. necessary to be...
Formation and growth of tetragonal lysozyme crystals at the interface protein solution with glass substrate have been studied. Morphology formed grown on a flat surface are given discussed. The influence second plate as well some differences in supersaturation is shown; four {101} pyramidal faces vanish, giving place hemisphere or "new" {001} face.
Herein, studies on the surface activities of newly synthesized l-Asp-based gemini surfactants, both nonionic and anionic, are presented. Conductometry, tensiometry, Langmuir-Blodgett (LB) film technique were applied for this purpose.
Biotechnological methods prevent the destruction of natural populations medicinal plants due to climate change and developing agriculture. This study evaluates effects in vitro pretreatment with two types silver-containing amino acid nanofibers (NF-1%Ag NF1-Ag salt) on drought tolerance ex soil-adapted Steviia rebaudiana Bertoni. The duration was five days. data suggested that studied during plant propagation enhanced stress manifested a smaller decrease biomass accumulation increase sugar...
Multiple sclerosis (MS) is an autoimmune disease of the central nervous system, characterized by inflammatory demyelination and neurodegeneration. Fampyridine (4-aminopyridine, 4-AP) a drug used for MS treatment as it readily passes through blood-brain barrier exhibiting anti-inflammatory effects. Its high toxicity, however, limits its wider application. To reduce 4-AP we have designed new peptides-containing derivatives showing about 150 times lower toxicity than 4-AP. This work aims to...
Background: Alzheimer’s disease (AD) and Multiple sclerosis (MS) lead to neurodegenerative processes negatively affecting millions of people worldwide. Their treatment is still difficult practically incomplete. One the most commonly used drugs against these diseases 4-aminopyridine. However, its use confined by high toxicity. Objectives: The aim this work obtain new peptide derivatives 4-aminopyridine with decreased toxicity compared Methods: Synthesis was conducted in solution using a...