A5001162205- Pancreatic function and diabetes
- T-cell and B-cell Immunology
- Drug-Induced Adverse Reactions
- Immune Cell Function and Interaction
- Diabetes and associated disorders
- CAR-T cell therapy research
- Xenotransplantation and immune response
- Food Allergy and Anaphylaxis Research
- Diabetes Management and Research
- Liver physiology and pathology
- Urticaria and Related Conditions
- Mesenchymal stem cell research
- SARS-CoV-2 and COVID-19 Research
- Tissue Engineering and Regenerative Medicine
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Allergic Rhinitis and Sensitization
- Classical Antiquity Studies
- Liver Disease Diagnosis and Treatment
- Renal Transplantation Outcomes and Treatments
- Autoimmune Bullous Skin Diseases
- Immunotherapy and Immune Responses
- Polyomavirus and related diseases
- Cannabis and Cannabinoid Research
- Organ Transplantation Techniques and Outcomes
- CRISPR and Genetic Engineering
University of Lausanne
2017-2025
University Hospital of Lausanne
2018-2025
University of Geneva
2010-2024
University of California, San Francisco
2018-2021
Hôpital de l'enfance
2021
Hôpital Orthopédique de la Suisse Romande
2021
University Hospital of Geneva
2010-2020
Universidad Católica de Santa Fe
2019
Institution Genevoise de Maintien à Domicile
2017
YD Global Life Science (South Korea)
2015
It is generally admitted that the endocrine cell organization in human islets different from of rodent islets. However, a clear description islet architecture has not yet been reported. The aim this work was to describe our observations on arrangement cells.Human pancreas specimens and isolated were processed for histology. Sections analyzed by fluorescence microscopy after immunostaining hormones endothelial cells.In small (40-60 mum diameter), beta-cells had core position, alpha-cells...
Mesenchymal stem cell (MSC) transplantation was shown to be effective for the treatment of liver fibrosis, but mechanisms action are not yet fully understood. We transplanted encapsulated human MSCs in two mouse models fibrosis determine behind protective effect.Human bone marrow-derived were microencapsulated novel alginate-polyethylene glycol microspheres. In vitro, we analyzed effect MSC-conditioned medium on activation hepatic stellate cells and viability, proliferation, cytokine...
Anti-CD19 chimeric antigen receptor (CD19-CAR)-engineered T cells are approved therapeutics for malignancies. The impact of the hinge domain (HD) and transmembrane (TMD) between extracellular antigen-targeting CARs intracellular signaling modalities has not been systemically studied. In this study, a series 19-CARs differing only by their HD (CD8, CD28, or IgG4) TMD (CD8 CD28) was generated. containing CD28-TMD, but CD8-TMD, formed heterodimers with endogenous CD28 in human cells, as shown...
Infusion of regulatory T cells (Tregs) engineered with a chimeric antigen receptor (CAR) targeting donor-derived human leukocyte (HLA) is promising strategy to promote transplant tolerance. Here, we describe an anti-HLA-A2 CAR (A2-CAR) generated by grafting the complementarity-determining regions (CDRs) monoclonal antibody into framework Herceptin 4D5 single-chain variable fragment and fusing it CD28-ζ signaling domain. The CDR-grafted A2-CAR maintained specificity original antibody. We then...
Although gut and lymph node (LN) memory CD4 T cells represent major HIV simian immunodeficiency virus (SIV) tissue reservoirs, the study of role dendritic (DCs) in persistence has long been limited to blood due difficulties access lymphoid samples. In this study, we show that LN migratory resident DC subpopulations harbor distinct phenotypic transcriptomic profiles. Interestingly, both contain intact provirus inducible replication-competent despite expression antiviral restriction factor...
Protein-protein interactions (PPIs) are at the core of all key biological processes. However, complexity structural features that determine PPIs makes their design challenging. We present BindCraft, an open-source and automated pipeline for
OBJECTIVE Streptozotocin (STZ) is the most widely used diabetogenic agent in animal models of islet transplantation. However, immunomodifying effects STZ and ensuing hyperglycemia on lymphocyte subsets, particularly T regulatory cells (Tregs), remain poorly understood. RESEARCH DESIGN AND METHODS This study evaluated how STZ-induced diabetes affects adaptive immunity consequences thereof allograft rejection murine skin The respective toxicity subsets was tested vitro. effect assessed...
It has been suggested that the age of human organ donors might influence islet isolation and transplantation outcome in a negative way due to decrease vivo function islets isolated from older donors.We retrospectively analyzed 332 isolations according donor age. We determined by yields, rates, [beta]-cell vitro. Transplanted patients were divided into two groups depending on (n=25 n=31 for <=45- >45-year-old donors, respectively). assessed graft C-peptide/glucose ratio, [beta] score,...
Background: Interleukin-1 (IL-1)β and IL-1 receptor antagonist (IL-1Ra) have been proposed as important mediators during chronic liver diseases. We aimed to determine whether the modulation of IL-1β signaling with IL-1Ra impacts on fibrosis. Methods: assessed effects human hepatic stellate cells (HSC) in mouse models fibrosis induced by bile duct ligation (BDL) or carbon tetrachloride treatment (CCl-4). Results: Human HSCs treated had increased IL-1β, IL-1Ra, MMP-9 expressions vitro. reduced...
This cohort study examines the association of COVID-19 vaccine booster with chronic spontaneous urticaria in Swiss patients.
Treg therapies are being tested in clinical trials transplantation and autoimmune diseases, however, the impact of inflammation on Tregs remains controversial. We challenged human ex-vivo with pro-inflammatory cytokines IL-6 TNF α observed greatly enhanced proliferation stimulated by anti-CD3 anti-CD28 (aCD3/28) beads or CD28 superagonist (CD28SA). The cytokine-exposed maintained high expression FOXP3 HELIOS, demethylated enhancer, low IFNγ, IL-4, IL-17 secretion. Blocking receptor using...
Extended pancreatectomy is associated with the risk of surgical diabetes. Islet autotransplantation successful in prevention diabetes after pancreas resection for chronic pancreatitis (CP), insulin independence rates 50% at 1 year. The aim present study to demonstrate safety and efficiency islet extended left benign disease.Between 1992 2009, 25 patients underwent disease. Of these, 15 were operated focal lesions located neck (14 tumors traumatic pancreatic section), remainder being CP...
Systemic lupus erythematosus (SLE) is characterized by dysregulated humoral immunity, leading to the generation of autoreactive B cells that can differentiate both within and outside lymph node (LN) follicles. Here, we employed spatial transcriptomics multiplex imaging investigate follicular immune landscaping in situ transcriptomic profile LNs from SLE individuals. Our analysis revealed robust type I IFN plasma cell signatures compared reactive, control Cell deconvolution T subsets are...
In Switzerland, only scarce data are available on the prevalence and treatment of allergic rhinitis. Although presence AR symptoms in temporal relation to respective aeroallergen is indicative, still a substantial number affected individuals deemed underdiagnosed potentially undertreated. A national online survey was conducted for consecutive participants with medical practices irrespective diagnosis, therapy, or reason visit. Univariate multivariate regression analyses were performed, as...
Celiac disease, a gluten-sensitive enteropathy, demonstrates strong human leukocyte antigen (HLA) association, with more than 90% of patients carrying the HLA-DQ2.5 allotype. No therapy is available for condition except lifelong gluten-free diet. To address this gap, we explored therapeutic potential regulatory T cells (Tregs). By orthotopic replacement cell receptors (TCRs) through homology-directed repair, generated gluten-reactive HLA-DQ2.5-restricted CD4+ engineered (e) effector (Teffs)...
Type 1 diabetes (T1D) is marked by the overexpression of class I major histocompatibility complex (MHC) antigens in pancreatic islets, which are targeted islet-specific CD8+ T cells. Here, we aimed to improve regulatory cell (Treg) infiltration into islets redirecting their specificity toward I-restricted islet antigens. We functionally validated two public specific HLA-A2 (*02:01) restricted TCRs, one for ZnT8186-194 (clone D222D), second IGRP265-273 32) dual locus (TRAC/CD4)...
Chronic urticaria manifests as itchy, transient and migrating wheals and/or angioedema lasting more than 6 weeks. It can be classified spontaneous or inducible by physical factors. is secondary to mast cell activation due autoallergy (type I) autoimmunity IIb). While the initial work-up consists essentially in evaluating presence of biomarkers, it completed according comorbidities factors identified with history examination. Second generation antihistamines up four-times standard dose are...