A5032981298- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Genetic and Kidney Cyst Diseases
- Renal and related cancers
- Silk-based biomaterials and applications
- Additive Manufacturing and 3D Printing Technologies
- Pluripotent Stem Cells Research
- Cardiomyopathy and Myosin Studies
- Muscle activation and electromyography studies
- Epigenetics and DNA Methylation
- Microtubule and mitosis dynamics
- Nuclear Structure and Function
- Kruppel-like factors research
- Cancer, Hypoxia, and Metabolism
- Muscle Physiology and Disorders
- Collagen: Extraction and Characterization
- Assisted Reproductive Technology and Twin Pregnancy
- Cellular Mechanics and Interactions
- Cancer Research and Treatments
- Bone Tissue Engineering Materials
- Angiogenesis and VEGF in Cancer
- Ubiquitin and proteasome pathways
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cell Image Analysis Techniques
Friedrich-Alexander-Universität Erlangen-Nürnberg
2018-2025
University of Göttingen
2021
German Centre for Cardiovascular Research
2021
Universitätsklinikum Erlangen
2021
Arkana Laboratories
2020
3D-bioprinting is a promising technology to produce human tissues as drug screening tool or for organ repair. However, direct printing of living cells has proven difficult. Here, method presented directly 3D-bioprint induced pluripotent stem cell (hiPSC)-derived cardiomyocytes embedded in collagen-hyaluronic acid ink, generating centimeter-sized functional ring- and ventricle-shaped cardiac an accurate reproducible manner. The printed contain hiPSC-derived with well-organized sarcomeres...
Abstract During bioprinting, cells are suspended in a viscous bioink and extruded under pressure through small diameter printing needles. The combination of high needle exposes to considerable shear stress, which can lead cell damage death. Approaches monitor control stress-induced currently not well established. To visualize the effects printing-induced stress on plasma membrane integrity, we add FM 1-43 bioink, styryl dye that becomes fluorescent when bound lipid membranes, such as...
The switch from centrosomal microtubule-organizing centers (MTOCs) to non-centrosomal MTOCs during differentiation is poorly understood. Here, we identify AKAP6 as key component of the nuclear envelope MTOC. In rat cardiomyocytes, anchors proteins through its spectrin repeats, acting an adaptor between nesprin-1α and Pcnt or AKAP9. addition, AKAP9 form a protein platform tethering Golgi nucleus. Both exhibit MTOC activity utilizing either AKAP9, Pcnt-AKAP9, respectively. also required for...
Abstract The interplay between contractility and mechanosensing in striated muscle is important for tissue morphogenesis, load adaptation, disease progression, but remains poorly understood. Here, we investigate how contractile force generation neonatal rat cardiac C2C12 mouse skeletal micro-tissues depends on environmental stiffness. Micro-tissues self-assemble mature over one week flexible elastic pillars with adjustable stiffness that vary three orders of magnitude. Contractile forces are...
Tissue engineering enables the production of tissues and organ-like structures as models for drug testing mechanistical studies or functional replacements injured tissues. Available cytocompatible materials are limited in number, suffer from insufficient mechanical properties, cells interacting with them often cause construct shrinkage. As shape is important function, identifying cytocompatible, shrink-resistant a major aim. Here, it shown that hydrogels made interpenetrating networks...
Materials made of recombinant spider silk proteins are promising candidates for cardiac tissue engineering, and their suitability has so far been investigated utilizing primary rat cardiomyocytes. Herein, we expanded the tool box available variants demonstrated first time that human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes attach, contract, respond to pharmacological treatment using phenylephrine verapamil on explicit films. The hiPSC-cardiomyocytes contracted at least 14...
Abstract Cardiac tissue engineering is a promising strategy to generate human cardiac tissues for modeling diseases, screening therapeutic drugs, and repairing the injured heart. Yet, several issues remain be resolved including generation of with high cardiomyocyte density. Here, it shown that integration glycogen synthase kinase‐3 β inhibitor CHIR99021 in collagen I hydrogels promotes proliferation human‐induced pluripotent stem cell‐derived (hiPSC) cardiomyocytes post‐fabrication improving...
Human pluripotent stem cells (hPSCs) hold great promise for applications in cell therapy and drug screening the cardiovascular field. Bone morphogenetic protein 4 (BMP4) is key early cardiac mesoderm induction hPSC subsequent cardiomyocyte derivation. Small-molecular BMP4 mimetics may help to standardize derivation from hPSCs. Based on observations that chalcones can stimulate signaling pathways, we hypothesized their utility induction. To test this, set up a two-tiered strategy, (1)...
Abstract Background Cardiac tissue engineering is an effective strategy to generate tissues for drug testing and disease modelling as well cardiac repair. Tissues produced by casting show good functionality advanced maturation, but do not replicate the native architecture hierarchy. Additive manufacturing technologies, such 3D bioprinting, enable generation of hierarchically structured with complex geometries. This technology has been used previously models heart. However, these approaches...
Abstract The mechanical interplay between contractility and mechanosensing in striated muscles is of fundamental importance for tissue morphogenesis, load adaptation, disease progression, but remains poorly understood. In this study, we investigate the dependence contractile force generation cardiac skeletal muscle on environmental stiffness. Using vitro engineered micro-tissues that are attached to flexible elastic pillars, vary stiffness microenvironment over three orders magnitude study...
Abstract 3D-bioprinting is a promising technology to produce human tissues as drug screening tool or for organ repair. However, direct printing of living cells has proven difficult. Here, we present method directly 3D-bioprint induced pluripotent stem cell (hiPSC)-derived cardiomyocytes embedded in collagen-hyaluronic acid ink generating centimeter-sized functional ring- and ventricle-shaped cardiac an accurate reproducible manner. The printed contained hiPSC-derived with well-organized...
Abstract Motile cilia serve vital functions in development, homeostasis and regeneration. We recently demonstrated that TAp73 is an essential transcriptional regulator of respiratory multiciliogenesis. Here, we show expressed multiciliated cells (MCCs) diverse tissues. Analysis mutant animals revealed regulates Foxj1, Rfx2, Rfx3 , axonemal dyneins Dnali1 Dnai1 plays a pivotal role the generation MCCs male female reproductive ducts, contributes to fertility. However, function brain appears be...
Abstract Background/Introduction Cardiac tissue engineering is a promising strategy to generate human cardiac tissues for modelling diseases, screening therapeutic drugs, and repairing the injured heart. Yet, several issues remain be resolved including generation of with high cardiomyocyte density. Purpose Determining effects induction human-induced pluripotent stem cell-derived (hiPSC) proliferation post-fabrication. Methods hiPSCs were differentiated into cardiomyocytes, embedded or...