A5088308813- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- Immune responses and vaccinations
- SARS-CoV-2 and COVID-19 Research
- vaccines and immunoinformatics approaches
- Clostridium difficile and Clostridium perfringens research
- Gut microbiota and health
- Single-cell and spatial transcriptomics
- Immune Response and Inflammation
- Diet and metabolism studies
Babraham Institute
2018-2023
Abstract The magnitude and quality of the germinal center (GC) response decline with age, resulting in poor vaccine-induced immunity older individuals. A functional GC requires co-ordination multiple cell types across time space, particular its two functionally distinct compartments: light dark zones. In aged mice, there is CXCR4-mediated mislocalization T follicular helper (T FH ) cells to zone a compressed network dendritic (FDCs) zone. Here we show that localization critical for antibody...
Abstract Ageing is a complex multifactorial process associated with plethora of disorders, which contribute significantly to morbidity worldwide. One the organs affected by age gut. Age-dependent changes gut-associated microbiome have been linked increased frailty and systemic inflammation. This change in microbial composition occurs parallel decline function gut immune system; however, it not clear whether there causal link between two. Here we report that defective germinal centre reaction...
BackgroundThe spread of SARS-CoV-2 has caused a worldwide pandemic that affected almost every aspect human life. The development an effective COVID-19 vaccine could limit the morbidity and mortality by infection may enable relaxation social-distancing measures. Age is one most significant risk factors for poor health outcomes after infection; therefore, it desirable any new candidates elicit robust immune response in older adults.MethodsHere, we use in-depth immunophenotyping to characterize...
Germinal centres (GCs) are T follicular helper cell (Tfh)-dependent structures that form in response to vaccination, producing long-lived antibody secreting plasma cells and memory B protect against subsequent infection. With advancing age the GC Tfh declines, resulting impaired humoral immunity. We sought discover what underpins poor ageing whether it is possible correct it. Here, we demonstrate older people aged mice have differentiation upon vaccination. This deficit preceded by...
The failure to generate enduring humoral immunity after vaccination is a hallmark of advancing age. This can be attributed reduction in the germinal center (GC) response, which generates long-lived antibody-secreting cells that protect against (re)infection. Despite intensive investigation, primary cellular defect underlying impaired GCs aging has not been identified. Here, we used heterochronic parabiosis demonstrate GC formation was dictated by age lymph node (LN) microenvironment rather...
Abstract The spread of SARS-CoV-2 has caused a global pandemic that affected almost every aspect human life. development an effective COVID-19 vaccine could limit the morbidity and mortality by infection, may enable relaxation social distancing measures. Age is one most significant risk factors for poor health outcomes after therefore it desirable any new candidates should elicit robust immune response in older adults. Here, we test immunogenicity adenoviral vectored ChAdOx1 nCoV-19...
Abstract The failure to generate enduring humoral immunity after vaccination is a hallmark of advancing age. This can be attributed reduction in the germinal center response, which generates long-lived antibody-secreting cells that provide protection against (re)infection. Despite intensive investigation into effect age on lymphoid compartment, primary cellular defect causes impaired centers aging has not been identified. Herein we demonstrate reduces capacity center-associated stromal...
Affinity maturation, the progressive increase in serum Ab affinity after vaccination, is an essential process that contributes to effective humoral response against vaccines and infections. Germinal centers are key for because they where B cells undergo somatic hypermutation of their Ig genes dark zone before going through positive selection light via interactions with T follicular helper dendritic cells. In aged mice, maturation has been shown be impaired immunization, but whether...
Abstract Affinity maturation, the progressive increase in serum antibody affinity after vaccination, is an essential process that contributes to effective humoral response against vaccines and infections. Germinal centres (GCs) are key for as they where B cells undergo somatic hypermutation of their immunoglobulin genes dark zone, before going through positive selection light zone via interactions with T follicular helper dendritic cells. In aged mice, maturation has been shown be impaired,...
Abstract Vaccination generates long-lived antibody-mediated immunity against (re-)infection. This humoral is derived from memory B cells and antibody-secreting plasma via the germinal centre (GC) response. The magnitude quality of GC response declines with age, resulting in poor vaccine-induced older individuals, but causal factor/s this age-related decline are unknown. A functional requires co-ordination multiple cell types across time space, particular its two functionally distinct...